Human Mast Cell Chymase ELISA Kit

Instructions
Code CSB-E13757h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

CUSABIO’s Human Mast Cell Chymase ELISA Kit is a sandwich immunoassay specifically designed and validated for the quantitative detection of mast cell Chymase in the serum, cell culture supernates, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for cardiovascular research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay is based on the Sandwich-ELISA mechanism (the mast cell Chymase in the sample is sandwiched between the pre-coated mast cell Chymase antibody and Biotin-labeled mast cell Chymase antibody) and enzyme-substrate chromogenic reaction. The color intensity is positively related to the amount of mast cell Chymase bound in the initial step. The levels of mast cell Chymase in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

Mast cell chymase is a protease produced and released by activated mast cells. It is involved in multiple biological processes, including the conversion of angiotensin-I (Ang-I) into angiotensin-II (Ang-II), production of fibronectin and TGF-β from the extracellular matrix (ECM), processing of collagen for fibril formation from type I procollagen, and activation of matrix metalloproteinases (MMPs) from their zymogens and IL-1β, IL-18, endothelin-1, and endothelin-2 from their latent forms. Mast cell chymase also breaks down lipoproteins, facilitating the formation of macrophage foam cells. It also participates in collagen synthesis and tissue fibrosis, angiogenesis, and immunoglobulin molecule synthesis.

Target Name chymase 1, mast cell
Alternative Names Alpha-chymase ELISA Kit; Chymase 1 ELISA Kit; Chymase 1 mast cell ELISA Kit; chymase 1 preproprotein transcript E ELISA Kit; chymase 1 preproprotein transcript I ELISA Kit; Chymase ELISA Kit; Chymase; heart ELISA Kit; Chymase; mast cell ELISA Kit; CMA1 ELISA Kit; CMA1_HUMAN ELISA Kit; CYH ELISA Kit; CYM ELISA Kit; EC 3.4.21.39 ELISA Kit; Mast cell chymase 1 ELISA Kit; Mast cell protease 3 ELISA Kit; Mast cell protease 5 ELISA Kit; Mast cell protease I ELISA Kit; Mast cell protease III ELISA Kit; Mcp-5 ELISA Kit; MCP3P ELISA Kit; Mcpt5 ELISA Kit; MCT1 ELISA Kit; MGC119890 ELISA Kit; MGC119891 ELISA Kit; MMCP-5 ELISA Kit
Abbreviation CMA1
Uniprot No. P23946
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human mast cell chymase (CMA1) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %98
Range %94-103
1:2Average %90
Range %84-95
1:4Average %99
Range %94-104
1:8Average %89
Range %85-94
Recovery
The recovery of human mast cell chymase(CMA1) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-95
EDTA plasma (n=4)10195-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.735 2.789 2.762 2.656
52.397 2.385 2.391 2.285
2.51.832 1.869 1.851 1.745
1.251.256 1.241 1.249 1.143
0.6250.725 0.756 0.741 0.635
0.3120.453 0.468 0.461 0.355
0.1560.289 0.281 0.285 0.179
00.105 0.107 0.106  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human mast cell Chymase antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled mast cell Chymase antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Gene References into Functions
  1. These findings reveal the critical role of hypoxia in producing local angiotensin II by a lactate-chymase-dependent mechanism and highlight the importance of local angiotensin II in regulating radioresistance of hypoxic tumor cells. PMID: 28205588
  2. Cleavage of the alarmins by Human mast cell chymase and human neutrophil cathepsin G suggests a function in regulating excessive inflammation. PMID: 28053237
  3. activation of endothelial CLP/chymase may directly relate to the increased inflammatory phenotypic changes in the vascular system in women with preeclampsia PMID: 19494363
  4. Placenta-derived chymotrypsin-like protease contributes to the altered endothelial barrier function in preeclampsia. PMID: 19126871
  5. promoter single nucleotide polymorphism is not associated with Dengue Hemorrhagic Fever and Dengue Shock Syndrome in Vietnam and Philippines PMID: 25797204
  6. Our data demonstrated that mast cell chymase plays an important role in keloid formation through TGF-beta1/Smad signaling pathway. PMID: 25120737
  7. These data suggest a possible contribution of human chymase activation to LPS-induced mortality PMID: 24516344
  8. we present data indicating that MC tryptase and chymase contribute to the development of OSF and malignant transformation of the overlying epithelium. PMID: 24874976
  9. The polymorphisms and haplotypes of the CMA1 locus are associated with cardiac hypertrophy in male patients with symptomatic aortic stenosis. PMID: 24823657
  10. CMA1 gene single nucleotide polymorphisms is associated with essential hypertension. PMID: 25119908
  11. Significant association between the AG genotype of CMA1 A polymorphism and Angina Pectoris and Ventricular Extrasystoles was observed. PMID: 25102745
  12. The accumulated data in this review suggest that mast cells, their tryptases, and their chymases play important roles in tissue repair. PMID: 24507159
  13. demonstrate the generation of Pso p27 from SCCA1 with extracts from psoriatic scale and even more remarkably, the generation of Pso p27 from SCCA1 in the presence of mast cell associated chymase PMID: 24560885
  14. The present results indicated PRCP rs7104980 can be considered as a marker for EH and Hap3 GAGCACTAACA (PRCP) and Hap16 TTTA (CMA1) might be associated with essential hypertension in Chinese Han population. PMID: 22679278
  15. Purified chymase mixed with fragmented heparin derived from heparanase-expressing cells show greater release from collagen gels than the enzyme alone or mixed with macromolecular heparin derived from mock cells. PMID: 24344642
  16. Data indicate that the best Fynomer (the binding proteins derived from the Fyn SH3 domain) was found to bind chymase with a KD of 0.9 nM and koff of 6.6x10 (-4) s (-1) , and to selectively inhibit chymase activity with an IC 50 value of 2 nM. PMID: 22653218
  17. Both IgE and chymase associate with diabetes status. PMID: 22194960
  18. As mast cells are an important source of VEGF, tryptase, and chymase, these findings suggest that mast cell activation and mast cell-derived mediators participate in the development of Dengue hemorrhagic fever. PMID: 22363824
  19. Chymase rs1800875 polymorphism is associated with the progression of immunoglobulin A (IgA) nephropathy in Korean patients. PMID: 21150220
  20. a dominant role of cardiac chymase in the formation of Ang II from Ang-(1-12) PMID: 22180785
  21. Two different populations of mast cells were found in melanocytic skin; one expressing both chymase and tryptase and the other with tryptase only. PMID: 22102069
  22. mast cell-derived protein is present in uterine cervical carcinoma PMID: 21274549
  23. Polymorphism rs1800875 of CMA1 may be associated with serum IgE level in coronary heart disease subjects, but not with chymase level in normal coronary intimae PMID: 21796807
  24. human chymase contributes to blood glucose levels and mortality during the progression of diabetes PMID: 21786536
  25. short tandem repeat genetic polymorphism is associated with bronchial asthma in a Swiss cohort study PMID: 20736038
  26. Elevated maternal chymase activity and enhanced protease immunostaining in the maternal vessel endothelium may constitute the exacerbated inflammatory state and account for the increased vascular Ang II sensitivity in preeclampsia. PMID: 20670150
  27. Positions 143 (Arg) and 192 (Lys) in human mast cell chymase contribute to the strong preference for negatively charged amino acids at substrate position P2'. PMID: 20423454
  28. The levels of tryptase and chymase expression are greatly increased in human lung tissue of anaphylactic shock. PMID: 19697770
  29. The S1 primary specificity pocket defines substrate specificity of chymases. PMID: 11852067
  30. The mast cell chymase A3255 allele was shown to have an effect on HDL cholesterol metabolism. PMID: 12047032
  31. The local release of mast cell chymase has potentially profound effects on airway smooth muscle cell function by disruption of the cell-associated matrix and inhibition of epidermal growth factor-induced smooth muscle cell proliferation. PMID: 12097409
  32. Results suggest the additive effect of angiotensin I-converting enzyme (ACE) and heart chymase (CMA) gene polymorphisms on the increase in left ventricular mass in NIDDM patients. PMID: 12165749
  33. Chymase may play a role in heart remodeling by increasing Ang II formation and activating MMP-9, and the regulation of collagen I gene expression. PMID: 12359984
  34. Both the ACE and chymase-like enzyme activities in the aneurysmal aortae were significantly higher than those in the control aortae. PMID: 12484503
  35. Degradation of phospholipid transfer protein (PLTP) and PLTP-generated pre-beta-high density lipoprotein by this enzyme in mast cells impairs high affinity efflux of cholesterol from macrophage foam cells. PMID: 12531890
  36. Interactions among the loops bordering and defining the active site appear to influence both the zymogen and the activated conformations of chymase in this model. PMID: 12614156
  37. albumin is a substrate of human chymase PMID: 12815038
  38. new class of chymase inhibitor through a substituent analysis of MWP00965 chemically synthesized PMID: 14592513
  39. chymase depletes pre-beta-high density lipoprotein, which impairs ATP-binding cassette transporter A1- but not scavenger receptor class B type I-mediated lipid efflux to high density lipoprotein PMID: 14701812
  40. MCT1 immunoreactivity was visible in blood vessel walls as early as the 13th week of gestation mainly in the visual cortical plate and subplate. PMID: 14757520
  41. mast cell chymase-1 is unlikely to influence blood pressure levels in the Japanese population. PMID: 15106801
  42. Bladder fibrosis may be mediated by mast cell chymase stimulation of collagen synthesis. PMID: 15227657
  43. The synthesis of new potential inhibitors of human chymase is described PMID: 15449728
  44. Tryptase and chymase and protein levels were determined in mast cells in fibrosarcoma. PMID: 15638376
  45. mast cell chymase activates ERK and p38 probably through G-protein-coupled receptor, and the ERK but not p38 cascade may have a crucial role in chymase-induced migration of eosinophils PMID: 15919053
  46. A novel (TG)n(GA)m repeat polymorphism 254 bp downstream of the mast cell chymase (CMA1) gene is associated with atopic asthma and total serum IgE levels. PMID: 15924217
  47. Chymase-induced apoptosis of conjunctival epithelial cells represents anoikis, which is a slowly occurring apoptotic process induced by lack of adhesion to an extracellular matrix. PMID: 16020275
  48. Significant association between the CMA1 promoter polymorphism rs1800875 and atopic eczema supports the hypothesis that CMA1 serves as candidate gene for atopic eczema. PMID: 16134991
  49. activated human skin mast cells (MCs) convert CTAP-III into biologically active NAP-2 through proteolytic cleavage by released chymase. PMID: 16317101
  50. The CMA1 polymorphisms studied do not contribute to disease susceptibility in Japanese or Dutch sarcoidosis patients. PMID: 16446531

Show More

Hide All

Subcellular Location Secreted, Cytoplasmic granule
Protein Families Peptidase S1 family, Granzyme subfamily
Tissue Specificity Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
Database Links

HGNC: 2097

OMIM: 118938

KEGG: hsa:1215

STRING: 9606.ENSP00000250378

UniGene: Hs.135626

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1