Sample type: Serum
Sample species: Mouse
Sample dilution: No dilution
Review: We used \'ELISA kit for human mast cell chymase – Cusabio® - CSBE13757h\' for the research project - \'\'Assessing the Serum Chymase Level as an Early Predictor of Dengue Severity” at All India Institute of Medical Sciences, New Delhi, India. We used dengue positive human serum and assayed it for serum chymase levels. Our intra-assay variation – 7.2% (three samples of 10 ng/mL concentration were tested four times on one plate) and Inter-assay variation – 9.4% (Three samples of 10 ng/mL concentration were tested in three different plates). Finally, our research concluded - \'At an optimum cut-off value of 1.35 ng/mL, chymase had a positive likelihood ratio (LR) of 3.5 and a negative LR of 0.15, for predicting severe dengue.\'
This study was published in the Journal of Medical Virology (10.1002/jmv.26468) and the product of Cusabio is duly cited and acknowledged.