Human Meteorin-like protein(METRNL) ELISA kit

Instructions
Code CSB-EL013718HU
Size 96T,5×96T,10×96T

Product Details

Target Name meteorin, glial cell differentiation regulator-like
Alternative Names MGC99788,
Abbreviation METRNL
Uniprot No. Q641Q3
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human METRNL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-89  
1:2 Average % 90  
Range % 86-96  
1:4 Average % 95  
Range % 90-99  
1:8 Average % 93  
Range % 86-100  
Recovery
The recovery of human METRNL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 92-103  
EDTA plasma (n=4) 92 88-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.695 2.587 2.641 2.471  
5 2.079 2.136 2.108 1.938  
2.5 1.682 1.604 1.643 1.473  
1.25 1.266 1.169 1.218 1.048  
0.625 0.719 0.694 0.707 0.537  
0.312 0.399 0.427 0.413 0.243  
0.156 0.218 0.209 0.214 0.044  
0 0.173 0.167 0.170    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures (By similarity).
Subcellular Location Secreted
Protein Families Meteorin family
Tissue Specificity Highly expressed in the skeletal muscle, in subcutaneous adipose tissue, epididymal white adipose tissue depots and heart. Also expressed in brown adipose tissues and kidney.
Database Links

HGNC: 27584

OMIM: 616241

KEGG: hsa:284207

STRING: 9606.ENSP00000315731

UniGene: Hs.378821

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