METRNL Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA731035MA1HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate(30µg), A-431 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), colo205 whole cell lysate(30µg) All lanes: METRNL antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 3min Overlay Peak curve showing Hela cells stained with CSB-RA731035MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q641Q3

Target Names

METRNL

Alternative Names

Meteorin-like protein (Subfatin), METRNL

Species Reactivity

Human

Immunogen

Recombinant Human METRNL protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

18C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

METRNL, also known as Meteorin-like protein or Subfatin, has emerged as a compelling target in metabolic and immunological research. This secreted cytokine plays important roles in energy homeostasis, thermogenesis, and inflammatory responses, with studies linking it to adipose tissue browning, insulin sensitivity, and immune cell modulation. Its involvement in these interconnected pathways makes METRNL a valuable focus for researchers investigating metabolic disorders, obesity, and inflammatory conditions.

This recombinant monoclonal antibody, clone 18C10, offers the consistency and reliability that demanding experimental workflows require. Produced through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The human IgG1 isotype format and affinity-chromatography purification ensure high specificity for human METRNL detection.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis confirms robust detection of METRNL at the expected 34 kDa molecular weight across diverse human cell lines, including MCF-7 breast adenocarcinoma, A-431 epidermoid carcinoma, HEK293 embryonic kidney, and Colo205 colorectal adenocarcinoma cells, with optimal performance at dilutions between 1:500 and 1:2000. Flow cytometry validation in HeLa cells shows clear separation from isotype control, enabling intracellular detection of METRNL following fixation and permeabilization, with recommended dilutions of 1:50 to 1:200. ELISA compatibility further extends its utility for quantitative applications.

Whether you are exploring METRNL's role in adipocyte biology, investigating its immunomodulatory functions, or characterizing its expression patterns across cell types, this antibody provides a dependable tool for advancing your understanding of this metabolically significant protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures.

Gene References into Functions

downregulation of METRNL during adipogenesis and functional induction of increased proliferation in stromal vascular fraction cells with concomitant inhibition of adipocyte differentiation may result in hypertrophic AT accumulation PMID: 27748745
Overexpression of METRNL inhibited mineralized nodule formation. PMID: 27716826
Data suggest that there is no correlation between serum Metrnl levels and BMI (body mass index) in humans. PMID: 26307585
Results show that Subfatin is a novel adipokine regulated by adipogenesis and obesity, with tissue distribution different from its homologue Meteorin PMID: 24393292

Subcellular Location

Secreted.

Protein Families

Meteorin family

Tissue Specificity

Highly expressed in the skeletal muscle, in subcutaneous adipose tissue, epididymal white adipose tissue depots and heart. Also expressed in brown adipose tissues and kidney.

Database Links

HGNC: 27584

OMIM: 616241

KEGG: hsa:284207

STRING: 9606.ENSP00000315731

UniGene: Hs.378821

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address