Human Mucin 2(MUC2) ELISA Kit

The human Mucin 2 (MUC2) ELISA kit is essential for quantifying MUC2 levels in human serum, plasma, and cell culture supernatants. It detects concentrations from 3.12 ng/mL to 200 ng/mL, with a sensitivity of 0.78 ng/mL. Utilizing a sandwich method, this kit ensures accurate and reliable measurements. It takes about 1-5 hours to accomplish the detection and needs only 50-100 µL of the sample. Detection occurs at a wavelength of 450 nm. The kit's effectiveness is well-documented in various experimental studies, demonstrating its reliability for research purposes.

The human MUC2 is a critical mucin protein that plays a significant role in forming a protective mucous layer in the intestines [1]. MUC2 regulates the expression and antimicrobial activity of β-defensin 2 in the colon, highlighting its role in the immune response and defense mechanisms in the gastrointestinal tract [2]. Furthermore, MUC2 contributes to synthesizing the antimicrobial peptide cathelicidin in response to colitis, emphasizing its involvement in the innate defenses of the colon against pathogens [3].

References:
[1] Z. Jiang, T. Applegate, & A. Lossie, "Cloning, annotation and developmental expression of the chicken intestinal muc2 gene", Plos One, vol. 8, no. 1, p. e53781, 2013. https://doi.org/10.1371/journal.pone.0053781
[2] E. Cobo, F. Moreau, & K. Chadee, "Colonic muc2 mucin regulates the expression and antimicrobial activity of β-defensin 2", Mucosal Immunology, vol. 8, no. 6, p. 1360-1372, 2015. https://doi.org/10.1038/mi.2015.27
[3] E. Cobo, V. Kissoon‐Singh, R. Holani, & K. Chadee, "Muc2 mucin and butyrate contribute to the synthesis of the antimicrobial peptide cathelicidin in response to entamoeba histolytica- and dextran sodium sulfate-induced colitis", Infection and Immunity, vol. 85, no. 3, 2017. https://doi.org/10.1128/iai.00905-16