Human Mucin 2(MUC2) ELISA Kit

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Target Background

Name: Human Mucin 2(MUC2) ELISA Kit
Brand: CUSABIO
SKU: CSB-E13122h

Product Details

Target Name

mucin 2, oligomeric mucus/gel-forming

Alternative Names

Intestinal mucin 2 ELISA Kit; Intestinal mucin-2 ELISA Kit; MLP ELISA Kit; MUC 2 ELISA Kit; MUC-2 ELISA Kit; Muc2 ELISA Kit; MUC2_HUMAN ELISA Kit; Mucin 2 ELISA Kit; Mucin 2 intestinal ELISA Kit; Mucin 2 intestinal/tracheal ELISA Kit; Mucin 2 oligomeric mucus/gel forming ELISA Kit; Mucin 2 precursor ELISA Kit; Mucin 2 tracheal ELISA Kit; Mucin like protein ELISA Kit; Mucin-2 ELISA Kit; Mucin2 ELISA Kit; SMUC ELISA Kit

Abbreviation

MUC2

Uniprot No.

Q02817

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

3.12 ng/mL-200 ng/mL

Sensitivity

0.78 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	101
	Range %	99-105
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	85-94

Recovery

The recovery of human MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.531	2.481	2.506	2.364
100	2.032	1.961	1.997	1.855
50	1.448	1.401	1.425	1.283
25	0.876	0.823	0.850	0.708
12.5	0.597	0.623	0.610	0.468
6.25	0.391	0.402	0.397	0.255
3.12	0.233	0.217	0.225	0.083
0	0.144	0.139	0.142

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MUC2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled MUC2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human MUC2 ELISA Kit was designed for the quantitative measurement of Human MUC2 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .

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■ Q&A

Please provide then following information regarding Human Mucin 2, MUC2 ELISA Kit (CSB-E13122h)
1. Are the antibodies in the kit monoclonal or polyclonal ?
2. If the antibodies are polyclonal, please kindly let us know the HOST, antigen, and the purification method
(Affinity chromatography or protein A (G) etc.)
3. what is stop solution ? 1M Hydrochloric Acid ?
4. If the kit includes vaculovirus or other virus expressions,
please kindly let us know which items in the kit include it.
5. Please kindly advise the source of Standard. synthesis ? or Recombinant ? or Extraction ? or other ?
6. If it is recombinant, please kindly advise the host.
7. Please kindly let us know whether the antibody and the standard includes any preservative or not.
ex. thimerosal or sodium azide or 2-mercaptoethanol etc.

Thanks for your inquiry.
CSB-E13122h
Capture antibody is mouse monoclonal antibody and it is Protein A/G purified.
Detection antibody is goat polyclonal antibody and it is affinity purified..
Standard is 293 cell-derived recombinant protein.
This kit doesn't contain vaculovirus or other virus expressions；
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

Subcellular Location

Secreted.

Tissue Specificity

Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.

Database Links

HGNC: 7512

OMIM: 158370

STRING: 9606.ENSP00000415183

UniGene: Hs.315

Code	CSB-E13122h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.