Human Mucin 2(MUC2) ELISA Kit

Code CSB-E13122h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human MUC2 ELISA Kit was designed for the quantitative measurement of Human MUC2 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .

Target Name mucin 2, oligomeric mucus/gel-forming
Alternative Names Intestinal mucin 2 ELISA Kit; Intestinal mucin-2 ELISA Kit; MLP ELISA Kit; MUC 2 ELISA Kit; MUC-2 ELISA Kit; Muc2 ELISA Kit; MUC2_HUMAN ELISA Kit; Mucin 2 ELISA Kit; Mucin 2 intestinal ELISA Kit; Mucin 2 intestinal/tracheal ELISA Kit; Mucin 2 oligomeric mucus/gel forming ELISA Kit; Mucin 2 precursor ELISA Kit; Mucin 2 tracheal ELISA Kit; Mucin like protein ELISA Kit; Mucin-2 ELISA Kit; Mucin2 ELISA Kit; SMUC ELISA Kit
Abbreviation MUC2
Uniprot No. Q02817
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %87-96
1:2Average %101
Range %99-105
1:4Average %94
Range %90-98
1:8Average %89
Range %85-94
Recovery
The recovery of human MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8480-88
EDTA plasma (n=4)9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.531 2.481 2.506 2.364
1002.032 1.961 1.997 1.855
501.448 1.401 1.425 1.283
250.876 0.823 0.850 0.708
12.50.597 0.623 0.610 0.468
6.250.391 0.402 0.397 0.255
3.120.233 0.217 0.225 0.083
00.144 0.139 0.142  
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MUC2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled MUC2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Please provide then following information regarding Human Mucin 2, MUC2 ELISA Kit (CSB-E13122h)
1. Are the antibodies in the kit monoclonal or polyclonal ?
2. If the antibodies are polyclonal, please kindly let us know the HOST, antigen, and the purification method
(Affinity chromatography or protein A (G) etc.)
3. what is stop solution ? 1M Hydrochloric Acid ?
4. If the kit includes vaculovirus or other virus expressions,
please kindly let us know which items in the kit include it.
5. Please kindly advise the source of Standard. synthesis ? or Recombinant ? or Extraction ? or other ?
6. If it is recombinant, please kindly advise the host.
7. Please kindly let us know whether the antibody and the standard includes any preservative or not.
ex. thimerosal or sodium azide or 2-mercaptoethanol etc.

A:
Thanks for your inquiry.
CSB-E13122h
Capture antibody is mouse monoclonal antibody and it is Protein A/G purified.
Detection antibody is goat polyclonal antibody and it is affinity purified..
Standard is 293 cell-derived recombinant protein.
This kit doesn't contain vaculovirus or other virus expressions;
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
Subcellular Location Secreted.
Tissue Specificity Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Database Links

HGNC: 7512

OMIM: 158370

STRING: 9606.ENSP00000415183

UniGene: Hs.315

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