MUC2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA188971A0HU
Size	US$210
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Image	Overlay Peak curve showing HT29 cells stained with CSB-RA188971A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q02817

Target Names

MUC2

Alternative Names

Intestinal mucin 2 antibody; Intestinal mucin-2 antibody; MLP antibody; MUC 2 antibody; MUC-2 antibody; Muc2 antibody; MUC2_HUMAN antibody; Mucin 2 antibody; Mucin 2 intestinal antibody; Mucin 2 intestinal/tracheal antibody; Mucin 2 oligomeric mucus/gel forming antibody; Mucin 2 precursor antibody; Mucin 2 tracheal antibody; Mucin like protein antibody; Mucin-2 antibody; Mucin2 antibody; SMUC antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human MUC2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14H6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MUC2 serves as the principal gel-forming mucin in the intestinal tract, where it establishes the protective mucus barrier that separates luminal contents from the underlying epithelium. This secreted glycoprotein plays a fundamental role in maintaining intestinal homeostasis, and its dysregulation has been implicated in inflammatory bowel diseases, colorectal cancer progression, and mucinous tumor phenotypes. Understanding MUC2 expression patterns and regulation provides critical insights into gastrointestinal pathophysiology and mucosal immunity.

This recombinant monoclonal antibody, clone 14H6, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human MUC2 and produced using recombinant technology in rabbit host, this antibody delivers sequence-defined specificity with minimal lot-to-lot variation. The monoclonal nature ensures that every experiment draws from the same defined binding characteristics, supporting reliable data across longitudinal studies and multi-site collaborations.

Validation in flow cytometry demonstrates clear detection of MUC2 in HT29 cells, a well-established human colorectal adenocarcinoma line known for mucin production. The intracellular staining protocol, employing fixation and permeabilization to access this secreted protein, showed distinct signal separation from isotype control, confirming specific target recognition. Recommended dilutions for flow cytometry range from 1:50 to 1:200, providing flexibility to optimize signal intensity for your specific experimental conditions. The antibody is also validated for ELISA applications.

Supplied in a stabilized liquid format with glycerol for convenient long-term storage at -20°C or -80°C, this antibody supports researchers investigating mucosal biology, gastrointestinal cancers, inflammatory conditions, and epithelial barrier function.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

Subcellular Location

Secreted.

Tissue Specificity

Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.

Database Links

HGNC: 7512

OMIM: 158370

STRING: 9606.ENSP00000415183

UniGene: Hs.315

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Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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