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| Code | CSB-EQ01026975HU |
| Size | 96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 91 | ||||
| Range % | 87-98 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:80 | Average % | 94 | ||||
| Range % | 86-105 | |||||
| The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-103 | ||||
| EDTA plasma (n=4) | 94 | 87-100 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human NGAL/MMP-9 complex ELISA kit is suitable for quantitatively determining human NGAL/MMP-9 complex in multiple samples, including serum, plasma, saliva, cell culture supernates, or tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human NGAL/MMP-9 complex levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.
NGAL protects MMP-9 from proteolytic degradation and enhances its stability and enzymatic activities by binding and forming the NGAL/MMP-9 complex. The NGAL/MMP-9 complex is involved in the development and progression of cancer. NGAL/MMP-9 complex expression and activities have been detected in tissue and urine samples from patients with vascular anomalies and cancers. NGAL, MMP-9, and their complex NGAL/MMP-9 have been proposed as soluble biomarkers for numerous malignancies.
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