Human NGAL/MMP-9 complex ELISA kit

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Citations

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Human NGAL/MMP-9 complex ELISA kit

Citations (6) Protocol MSDS

Code	CSB-EQ01026975HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details
Citations (6)
Customer Reviews and Q&A

Target Name

NGAL/MMP-9 complex

Abbreviation

NGAL/MMP-9 complex

Species

Homo sapiens (Human)

Sample Types

serum, plasma, saliva, cell culture supernates, tissue homogenates

Detection Range

0.313 ng/mL-20 ng/mL

Sensitivity

0.029 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	91
	Range %	87-98
1:20	Average %	95
	Range %	90-102
1:40	Average %	93
	Range %	87-99
1:80	Average %	94
	Range %	86-105

Recovery

The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	94	87-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.955	2.004	1.980	1.857
10	1.637	1.629	1.633	1.510
5	1.204	1.217	1.211	1.088
2.5	0.894	0.907	0.901	0.778
1.25	0.573	0.582	0.578	0.455
0.625	0.416	0.424	0.420	0.297
0.313	0.281	0.270	0.276	0.153
0	0.124	0.121	0.123

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The human NGAL/MMP-9 complex ELISA kit is suitable for quantitatively determining human NGAL/MMP-9 complex in multiple samples, including serum, plasma, saliva, cell culture supernates, or tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human NGAL/MMP-9 complex levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

NGAL protects MMP-9 from proteolytic degradation and enhances its stability and enzymatic activities by binding and forming the NGAL/MMP-9 complex. The NGAL/MMP-9 complex is involved in the development and progression of cancer. NGAL/MMP-9 complex expression and activities have been detected in tissue and urine samples from patients with vascular anomalies and cancers. NGAL, MMP-9, and their complex NGAL/MMP-9 have been proposed as soluble biomarkers for numerous malignancies.

Systematic Review of Kidney Injury Biomarkers for the Evaluation of CKD of Uncertain Etiology SE Claudel,/,/
Applicability of Novel Urinary Biomarkers for the Assessment of Renal Injury in Selected Occupational Groups in Sri Lanka: A Comparative Study with Conventional Markers E Ekanayake,International journal of environmental research and public health,2022
Urinary biomarkers indicate pediatric renal injury among rural farming communities in Sri Lanka T Gunasekara,Scientific reports,2022
Urinary biomarkers of early renal injury in antiretroviral‐naïve HIV‐positive persons in Shanghai, China: comparison with the general population XQ Le,HIV medicine,2021
Evaluation of wound healing biomarkers of Interleukin 6 (IL-6), Vascular Endothelial Growth Factor (VEGF) and Matrix Metalloproteinases 9 (MMP-9) in post Lower … J Omar,Bangladesh Journal of Medical Science,2020
Biomarkers upon discontinuation of renal replacement therapy predict 60-ay survival and renal recovery in critically ill patients with acute kidney injury Tingting Yang.et al,Hemodialysis International,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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