Human Neuritin(NRN1) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

neuritin 1

Alternative Names

Neuritin 1 ELISA Kit; Neuritin ELISA Kit; NRN ELISA Kit; Nrn1 ELISA Kit; NRN1_HUMAN ELISA Kit; OTTHUMP00000015989 ELISA Kit; RP3 380B8.2 protein ELISA Kit

Abbreviation

NRN1

Uniprot No.

Q9NPD7

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NRN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	95
	Range %	88-99
1:4	Average %	93
	Range %	86-98
1:8	Average %	100
	Range %	96-107

Recovery

The recovery of human NRN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-89
EDTA plasma (n=4)	90	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.590	2.780	2.685	2.491
1000	2.271	2.289	2.280	2.086
500	1.825	1.876	1.851	1.657
250	1.384	1.397	1.391	1.197
125	0.827	0.865	0.846	0.652
62.5	0.581	0.604	0.593	0.399
31.25	0.357	0.368	0.363	0.169
0	0.196	0.192	0.194

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human NRN1 ELISA Kit was designed for the quantitative measurement of Human NRN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

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Target Background

Function
(From Uniprot)

Promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells.

Gene References into Functions

NRN1 is expressed more extensively in melanoma than in normal melanocytes and healthy tissue. Secreted NRN1 seems to play a role also in earlier phases of melanoma development as we can discover NRN1 over-expression to be associated to primary melanoma PMID: 27901477
results suggest that DTNBP1 and NRN1 genes show a joint effect on the risk for schizophrenia spectrum disorders. Although the precise mechanism underlying this effect is unclear, the fact that these genes have been involved in synaptic maturation, connectivity and glutamate signalling suggests that our findings could be of value as a link to the schizophrenia aetiology. PMID: 27855309
NRN1 is associated with depressive symptoms and executive function in a non-clinical sample. Our results also suggest that the role of NRN1 seems to be modulated by BDNF. PMID: 28107668
On analysis of the expression of NRN1 in SMA patients for the first time, NRN1 could be a potential modifier gene PMID: 27279027
Ca(2+)/calcineurin (CaN)/nuclear factor of activated T-cells (NFAT) c4 axis is required for neuritin-induced Kv4.2 transcriptional expression and potentiation of IA densities in cerebellum granule neurons. PMID: 27307045
(i) NRN1 variability is a shared risk factor for both schizophrenia-spectrum disorders (SSD) and bipolar disorders (BPD), (ii) NRN1 may have a selective impact on age at onset and intelligence in SSD. PMID: 26700405
Data indicate that neuritin not only plays an important role in the nervous system but also has an effect on the migration, senescence, proliferation, and viability of stem cells PMID: 26208391
Neuritin is reduced in the brains of Alzheimer's disease (AD) patients. PMID: 25101829
MiR-204 promotes apoptosis in oxidative stress-induced rat Schwann cells by suppressing neuritin expression PMID: 25036738
we found neuritin is overexpressed in astrocytoma, which may be an important factor in tumorigenesis and progression of astrocytoma PMID: 20405246
Data show that SMN and HuD form a complex in spinal motor axons, and that both interact with cpg15 mRNA in neurons. PMID: 21652774
NRN1 polymorphisms have roles in fluid intelligence in schizophrenia PMID: 19569075
CPG15 and CPG15-2 perform similar cellular functions but may play distinct roles in vivo through their cell-type- and tissue-specific transcriptional regulation PMID: 18265009

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.

Protein Families

Neuritin family

Database Links

HGNC: 17972

OMIM: 607409

KEGG: hsa:51299

STRING: 9606.ENSP00000244766

UniGene: Hs.103291

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Code	CSB-EL016088HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.