Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by by a cell proliferation assay using rat C6 cells is less than 25 ng/ml, corresponding to a specific activity of >4.0x10⁴ IU/mg.

Target Names

NRN1

Uniprot No.

Q9NPD7

Research Area

Cell Biology

Alternative Names

Neuritin 1; Neuritin; NRN; Nrn1; NRN1_HUMAN; OTTHUMP00000015989; RP3 380B8.2 protein

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

28-115aa

Complete Sequence

AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN

Mol. Weight

9.7 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Neuritin (NRN1) is a GPI-anchored neurotrophic factor that promotes neurite outgrowth and synaptic plasticity, making it a key target in neuronal survival and differentiation research. This tag-free recombinant human NRN1 fragment (residues 28–115) encompasses the mature secreted domain and demonstrates robust biological potency, with an ED50 below 25 ng/mL in a rat C6 cell proliferation assay, corresponding to a specific activity exceeding 4.0 × 10⁴ IU/mg. The E. coli expression system provides a non-glycosylated protein suitable for receptor-ligand binding studies via SPR or ELISA, where the absence of variable glycan modifications supports more reproducible quantitative readouts. Purity above 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based proliferation and survival assays, neuronal differentiation studies, and use as a calibration standard in antibody characterization workflows.

Target Background

Function

Promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells.

Gene References into Functions

NRN1 is expressed more extensively in melanoma than in normal melanocytes and healthy tissue. Secreted NRN1 seems to play a role also in earlier phases of melanoma development as we can discover NRN1 over-expression to be associated to primary melanoma PMID: 27901477
results suggest that DTNBP1 and NRN1 genes show a joint effect on the risk for schizophrenia spectrum disorders. Although the precise mechanism underlying this effect is unclear, the fact that these genes have been involved in synaptic maturation, connectivity and glutamate signalling suggests that our findings could be of value as a link to the schizophrenia aetiology. PMID: 27855309
NRN1 is associated with depressive symptoms and executive function in a non-clinical sample. Our results also suggest that the role of NRN1 seems to be modulated by BDNF. PMID: 28107668
On analysis of the expression of NRN1 in SMA patients for the first time, NRN1 could be a potential modifier gene PMID: 27279027
Ca(2+)/calcineurin (CaN)/nuclear factor of activated T-cells (NFAT) c4 axis is required for neuritin-induced Kv4.2 transcriptional expression and potentiation of IA densities in cerebellum granule neurons. PMID: 27307045
(i) NRN1 variability is a shared risk factor for both schizophrenia-spectrum disorders (SSD) and bipolar disorders (BPD), (ii) NRN1 may have a selective impact on age at onset and intelligence in SSD. PMID: 26700405
Data indicate that neuritin not only plays an important role in the nervous system but also has an effect on the migration, senescence, proliferation, and viability of stem cells PMID: 26208391
Neuritin is reduced in the brains of Alzheimer's disease (AD) patients. PMID: 25101829
MiR-204 promotes apoptosis in oxidative stress-induced rat Schwann cells by suppressing neuritin expression PMID: 25036738
we found neuritin is overexpressed in astrocytoma, which may be an important factor in tumorigenesis and progression of astrocytoma PMID: 20405246
Data show that SMN and HuD form a complex in spinal motor axons, and that both interact with cpg15 mRNA in neurons. PMID: 21652774
NRN1 polymorphisms have roles in fluid intelligence in schizophrenia PMID: 19569075
CPG15 and CPG15-2 perform similar cellular functions but may play distinct roles in vivo through their cell-type- and tissue-specific transcriptional regulation PMID: 18265009

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.

Protein Families

Neuritin family

Database Links

HGNC: 17972

OMIM: 607409

KEGG: hsa:51299

STRING: 9606.ENSP00000244766

UniGene: Hs.103291

Code	CSB-AP002941HU
Abbreviation	Recombinant Human NRN1 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Human Neuritin protein (NRN1) (Active)

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Target Background

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