Human Neuropilin-2(NRP2) ELISA kit

Code CSB-EL016092HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O60462
Lead Time 7-14 working days
Abbreviation NRP2
Target Name neuropilin 2
Alias MGC126574, NP2, NPN2, PRO2714, VEGF165R2, OTTHUMP00000206355|neuropilin-2a(17)|neuropilin-2a(22)|neuropilin-2b(0)|receptor for VEGF165 and semaphorins class3|vascular endothelial growth factor-165 r
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C} and SEMA3F protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F}, and interacts with vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
HGNC 7751
RGD 620999
MGI 1858510
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NRP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 89-97  
1:2 Average % 96  
Range % 91-102  
1:4 Average % 92  
Range % 83-96  
1:8 Average % 86  
Range % 80-91  
Recovery
The recovery of human NRP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 103 98-107  
EDTA plasma (n=4) 97 92-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.540 2.432 2.486 2.375  
10 1.899 2.044 1.972 1.861  
5 1.232 1.276 1.254 1.143  
2.5 0.682 0.675 0.679 0.568  
1.25 0.386 0.374 0.380 0.269  
0.625 0.271 0.282 0.277 0.166  
0.312 0.184 0.186 0.185 0.074  
0 0.110 0.112 0.111    
References
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Function High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.
Subcellular Location Membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform s9: Secreted
Protein Families Neuropilin family
Database Links

HGNC: 8005

OMIM: 602070

KEGG: hsa:8828

STRING: 9606.ENSP00000353582

UniGene: Hs.471200

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