Human Neurotrophin 4,NT-4 ELISA KIT

Code CSB-E04689h
Size 96T,5×96T,10×96T
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Product Details

Target Name
neurotrophin 4
Alternative Names
GLC10 ELISA Kit; GLC1O ELISA Kit; Neurotrophic factor 4 ELISA Kit; Neurotrophic factor 5 ELISA Kit; Neurotrophin 4 ELISA Kit; Neurotrophin 4/5 ELISA Kit; Neurotrophin 5 (neurotrophin 4/5) ELISA Kit; Neurotrophin 5 ELISA Kit; Neurotrophin-4 ELISA Kit; Neurotrophin-5 ELISA Kit; Neutrophic factor 4 ELISA Kit; Neutrophic factor 5 ELISA Kit; NT 4 ELISA Kit; NT 4/5 ELISA Kit; NT 5 ELISA Kit; NT-4 ELISA Kit; NT-5 ELISA Kit; NT4 ELISA Kit; NT4/5 ELISA Kit; NT4P ELISA Kit; NT5 ELISA Kit; NTF4 ELISA Kit; NTF4_HUMAN ELISA Kit; NTF5 ELISA Kit
Abbreviation
NTF4
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %88-95
1:2Average %103
Range %99-106
1:4Average %93
Range %87-96
1:8Average %97
Range %93-100
Recovery
The recovery of human NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-97
EDTA plasma (n=4)9692-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.160 2.145 2.153 2.013
201.676 1.740 1.708 1.568
101.208 1.188 1.198 1.058
50.862 0.826 0.844 0.704
2.50.602 0.616 0.609 0.469
1.250.442 0.434 0.438 0.298
0.6250.296 0.278 0.287 0.147
00.142 0.138 0.140  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human NTF4 ELISA Kit was designed for the quantitative measurement of Human NTF4 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

Citations

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Target Background

Function
(From Uniprot)
Target-derived survival factor for peripheral sensory sympathetic neurons.
Gene References into Functions
  1. Elevated intra-amniotic levels of docosahexaenoic acid and arachidonic acid in the mid-trimester are correlated with increased NT4 concentrations. PMID: 24607879
  2. The NTF4 variants p.Gly157Ala and p.Ala182Val have been shown to be functional mutations, occurring in 2 of a total of 720 Chinese primary open-angle glaucoma patients. PMID: 22815630
  3. NT-4/5 expression is associated with atrophy of the brain-parenchymal fraction measured by magnetic resonance imaging methods in patients with relapsing-remitting multiple sclerosis. PMID: 22036954
  4. Suggest that a dysregulated TrkB/NT4/5 axis may contribute to several of the pathological lesions associated with pulmonary fibrosis, including type 2 alveolar cell hyperplasia and the proliferation of fibroblasts. PMID: 21330466
  5. neurotrophin 4 contributes to breast cancer cell survival and can serve as prospective target to inhibit tumor growth PMID: 21350004
  6. In vitro follicular assembly is significantly increased in fetal ovaries cultured with NT-4. PMID: 20447632
  7. Identification of a single mutation in our study suggests that NTF4 mutations are a rare cause of primary open-angle glaucoma (0.6%, 95%CI 0.02%-3.16%) in Chinese people. PMID: 20806036
  8. present data indicate a lack of involvement of variations in NTF4, VAV2, and VAV3 with glaucoma pathogenesis in an Indian population. PMID: 20463313
  9. Expression of human NTF4 was unchanged during gestation in the developping ovary. PMID: 20175187
  10. No evidence of association of heterozygous NTF4 mutations in patients with primary open-angle glaucoma. PMID: 20215012
  11. A mechanism to understand the defect associated with variant BDNF and provide a framework two ligands for BDNF and NT-4. PMID: 15987945
  12. Re-expression of the p75NTR appears to partially reverse de-differentiation of prostate cancer cells by up-regulating the expression of CRABPI for localized sequestration of retinoids. PMID: 16316409
  13. Results revealed the expression of NT-4/5 mainly in oocytes and, in a minority of samples, also in the granulosa cells (GCs). PMID: 16648150
  14. These novel data demonstrate that neurotrophins influence ASM [Ca(2+)](i) and force regulation and suggest a potential role for neurotrophins in airway diseases PMID: 16648236
  15. Neurotrophin 4/5 plays a role in the proliferation and differentiation of periodontal ligament cells. PMID: 18980528
  16. Data show that the levels of NT-4/5 levels are significantly higher in bipolar disorder patients than in controlsNT-4/5. immunocontent alterations in bipolar disorder. PMID: 19081579
  17. NTF4 mutations impair neurotrophin-4 signaling in patients with primary open-angle glaucoma and lead to decreased activation of TrkB PMID: 19765683

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Involvement in disease
Glaucoma 1, open angle, O (GLC1O)
Subcellular Location
Secreted.
Protein Families
NGF-beta family
Tissue Specificity
Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.
Database Links

HGNC: 8024

OMIM: 162662

KEGG: hsa:4909

STRING: 9606.ENSP00000301411

UniGene: Hs.266902

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