Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of >2.0x10⁴ IU/mg

Target Names

NTF4

Uniprot No.

P34130

Research Area

Neuroscience?

Alternative Names

GLC10; GLC1O; Neurotrophic factor 4; Neurotrophic factor 5; Neurotrophin 4; Neurotrophin 4/5; Neurotrophin 5 (neurotrophin 4/5); Neurotrophin 5; Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT 4; NT 4/5; NT 5; NT-4; NT-5; NT4; NT4/5; NT4P; NT5; NTF4; NTF4_HUMAN; NTF5

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

81-210aa

Complete Sequence

M+GVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A

Mol. Weight

14.1 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 5.5

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Neurotrophin-4 signals through the TrkB receptor to promote neuronal survival, differentiation, and synaptic plasticity, making it a critical tool for investigating neurotrophic signaling pathways. This tag-free recombinant human NTF4 encompasses the full mature protein (residues 81–210) and demonstrates robust biological potency, with an ED50 below 50 ng/mL in a choline acetyltransferase induction assay using rat basal forebrain septal cultures, corresponding to a specific activity exceeding 2.0 × 10⁴ IU/mg. These validated activity data support use in neuronal survival and differentiation studies, TrkB receptor-ligand binding and competition assays via ELISA or SPR, and stem cell differentiation protocols requiring defined neurotrophic stimulation. Produced in *E. coli* — appropriate for NTF4, which is not natively glycosylated — this protein provides purity greater than 97% by SDS-PAGE alongside endotoxin levels below 1.0 EU/μg, satisfying the quality criteria typical for sensitive primary cell culture systems and *in vivo* neuroregeneration models.

Target Background

Function

Target-derived survival factor for peripheral sensory sympathetic neurons.

Gene References into Functions

Elevated intra-amniotic levels of docosahexaenoic acid and arachidonic acid in the mid-trimester are correlated with increased NT4 concentrations. PMID: 24607879
The NTF4 variants p.Gly157Ala and p.Ala182Val have been shown to be functional mutations, occurring in 2 of a total of 720 Chinese primary open-angle glaucoma patients. PMID: 22815630
NT-4/5 expression is associated with atrophy of the brain-parenchymal fraction measured by magnetic resonance imaging methods in patients with relapsing-remitting multiple sclerosis. PMID: 22036954
Suggest that a dysregulated TrkB/NT4/5 axis may contribute to several of the pathological lesions associated with pulmonary fibrosis, including type 2 alveolar cell hyperplasia and the proliferation of fibroblasts. PMID: 21330466
neurotrophin 4 contributes to breast cancer cell survival and can serve as prospective target to inhibit tumor growth PMID: 21350004
In vitro follicular assembly is significantly increased in fetal ovaries cultured with NT-4. PMID: 20447632
Identification of a single mutation in our study suggests that NTF4 mutations are a rare cause of primary open-angle glaucoma (0.6%, 95%CI 0.02%-3.16%) in Chinese people. PMID: 20806036
present data indicate a lack of involvement of variations in NTF4, VAV2, and VAV3 with glaucoma pathogenesis in an Indian population. PMID: 20463313
Expression of human NTF4 was unchanged during gestation in the developping ovary. PMID: 20175187
No evidence of association of heterozygous NTF4 mutations in patients with primary open-angle glaucoma. PMID: 20215012
A mechanism to understand the defect associated with variant BDNF and provide a framework two ligands for BDNF and NT-4. PMID: 15987945
Re-expression of the p75NTR appears to partially reverse de-differentiation of prostate cancer cells by up-regulating the expression of CRABPI for localized sequestration of retinoids. PMID: 16316409
Results revealed the expression of NT-4/5 mainly in oocytes and, in a minority of samples, also in the granulosa cells (GCs). PMID: 16648150
These novel data demonstrate that neurotrophins influence ASM [Ca(2+)](i) and force regulation and suggest a potential role for neurotrophins in airway diseases PMID: 16648236
Neurotrophin 4/5 plays a role in the proliferation and differentiation of periodontal ligament cells. PMID: 18980528
Data show that the levels of NT-4/5 levels are significantly higher in bipolar disorder patients than in controlsNT-4/5. immunocontent alterations in bipolar disorder. PMID: 19081579
NTF4 mutations impair neurotrophin-4 signaling in patients with primary open-angle glaucoma and lead to decreased activation of TrkB PMID: 19765683

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Involvement in disease

Glaucoma 1, open angle, O (GLC1O)

Subcellular Location

Secreted.

Protein Families

NGF-beta family

Tissue Specificity

Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

Database Links

HGNC: 8024

OMIM: 162662

KEGG: hsa:4909

STRING: 9606.ENSP00000301411

UniGene: Hs.266902

Code	CSB-AP002831HU
Abbreviation	Recombinant Human NTF4 protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Human Neurotrophin-4 protein (NTF4) (Active)

Product Details

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Target Background

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