Human Neutrophil defensin 3(DEFA3) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

defensin, alpha 3, neutrophil-specific

Alternative Names

DEFA3 ELISA Kit; DEF3Neutrophil defensin 3 ELISA Kit; Defensin ELISA Kit; alpha 3 ELISA Kit; HNP-3 ELISA Kit; HP-3 ELISA Kit; HP3) [Cleaved into: HP 3-56; Neutrophil defensin 2 ELISA Kit; HNP-2 ELISA Kit; HP-2 ELISA Kit; HP2)] ELISA Kit

Abbreviation

DEFA3

Uniprot No.

P59666

Species

Homo sapiens (Human)

Sample Types

serum, plasma,tissue homogenates

Detection Range

1.87 ng/mL-120 ng/mL

Sensitivity

0.47 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	92
	Range %	84-96
1:4	Average %	94
	Range %	85-99
1:8	Average %	98
	Range %	90-102

Recovery

The recovery of human DEFA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	89-104
EDTA plasma (n=4)	98	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
120	1.875	1.897	1.886	1.762
60	1.412	1.476	1.444	1.320
30	0.907	0.928	0.918	0.794
15	0.552	0.545	0.549	0.425
7.5	0.359	0.344	0.352	0.228
3.75	0.245	0.241	0.243	0.119
1.87	0.185	0.179	0.182	0.058
0	0.125	0.123	0.124

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Related Products

Target Background

Function
(From Uniprot)

Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.

Gene References into Functions

High HNP3 expression is associated with IgA Nephropathy. PMID: 27563166
Elevated DEFA3 levels in diabetes are independent of DEFA3 copy numbers. PMID: 25083086
DEFA3 encodes an antibacterial peptide that is bacteriocidal against S. aureus, E. coli, and P. aeruginosa. PMID: 2997278
alpha-Defensin (DEFA3)was the third most differentially overexpressed gene and may be related to the onset of Bell's palsy and Ramsay Hunt Syndrome. PMID: 22737966
The total amount of gingival crevicular fluid human neutrophil peptide 3 were not different among periodontitis, gingivitis, and health control groups; there was no correlation with clinical periodontal parameters. PMID: 20151808
BPI and HNP 1-3 are accumulated in the synovial cavity of patients with rheumatoid arthritis PMID: 12913926
Aerobic bacteria were 100% susceptible to HBD-2 and HBD-3, whereas only 21.4 and 50% of the anaerobes were susceptible to HBD-2 and HBD-3. PMID: 15004048
HNP-3 is a potentially important regulator of neovascularizaiton, suggesting a new link between inflammation and angiogenesis. PMID: 15208269
We have tested the DEFA3 absence in 697 samples from different human populations. The proportion of subjects lacking DEFA3 varies from 10% to 37%, depending on the population tested, suggesting differences in innate immune function between populations. PMID: 17214878
Clostridium difficile toxin B interacts with high affinity with HNP-3 which may provide a defense mechanism against clostridial glucosylating cytotoxins. PMID: 18435932
Upregulation of HNP3 is associated with colorectal adenomas and carcinomas. PMID: 18957723
Salivary HNP-3concentrations increased following exercise PMID: 19263072
DEFA3 was upregulated in IPF patients with acute exacerbation PMID: 19363140
Studies indicate alpha-defensin, the products of neutrophils wase upregulated at the mRNA and protein levels in SLE patients. PMID: 19758174
Plasma levels of alpha-defensins 1-3 are an indicator of neutrophil activation in pregnant and post-partum women. PMID: 17845323

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Subcellular Location

Secreted.

Protein Families

Alpha-defensin family

Database Links

HGNC: 2762

OMIM: 604522

KEGG: hsa:1668

STRING: 9606.ENSP00000328359

UniGene: Hs.654448

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Code	CSB-EL006655HU
Size	96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.