Human Neutrophil defensin 3(DEFA3) ELISA kit

Instructions
Code CSB-EL006655HU
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name defensin, alpha 3, neutrophil-specific
Alternative Names DEFA3 ELISA Kit; DEF3Neutrophil defensin 3 ELISA Kit; Defensin ELISA Kit; alpha 3 ELISA Kit; HNP-3 ELISA Kit; HP-3 ELISA Kit; HP3) [Cleaved into: HP 3-56; Neutrophil defensin 2 ELISA Kit; HNP-2 ELISA Kit; HP-2 ELISA Kit; HP2)] ELISA Kit
Abbreviation DEFA3
Uniprot No. P59666
Species Homo sapiens (Human)
Sample Types serum, plasma,tissue homogenates
Detection Range 1.87 ng/mL-120 ng/mL
Sensitivity 0.47 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %88-101
1:2Average %92
Range %84-96
1:4Average %94
Range %85-99
1:8Average %98
Range %90-102
Recovery
The recovery of human DEFA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10089-104
EDTA plasma (n=4)9890-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1201.875 1.897 1.886 1.762
601.412 1.476 1.444 1.320
300.907 0.928 0.918 0.794
150.552 0.545 0.549 0.425
7.50.359 0.344 0.352 0.228
3.750.245 0.241 0.243 0.119
1.870.185 0.179 0.182 0.058
00.125 0.123 0.124
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.
Gene References into Functions
  1. High HNP3 expression is associated with IgA Nephropathy. PMID: 27563166
  2. Elevated DEFA3 levels in diabetes are independent of DEFA3 copy numbers. PMID: 25083086
  3. DEFA3 encodes an antibacterial peptide that is bacteriocidal against S. aureus, E. coli, and P. aeruginosa. PMID: 2997278
  4. alpha-Defensin (DEFA3)was the third most differentially overexpressed gene and may be related to the onset of Bell's palsy and Ramsay Hunt Syndrome. PMID: 22737966
  5. The total amount of gingival crevicular fluid human neutrophil peptide 3 were not different among periodontitis, gingivitis, and health control groups; there was no correlation with clinical periodontal parameters. PMID: 20151808
  6. BPI and HNP 1-3 are accumulated in the synovial cavity of patients with rheumatoid arthritis PMID: 12913926
  7. Aerobic bacteria were 100% susceptible to HBD-2 and HBD-3, whereas only 21.4 and 50% of the anaerobes were susceptible to HBD-2 and HBD-3. PMID: 15004048
  8. HNP-3 is a potentially important regulator of neovascularizaiton, suggesting a new link between inflammation and angiogenesis. PMID: 15208269
  9. We have tested the DEFA3 absence in 697 samples from different human populations. The proportion of subjects lacking DEFA3 varies from 10% to 37%, depending on the population tested, suggesting differences in innate immune function between populations. PMID: 17214878
  10. Clostridium difficile toxin B interacts with high affinity with HNP-3 which may provide a defense mechanism against clostridial glucosylating cytotoxins. PMID: 18435932
  11. Upregulation of HNP3 is associated with colorectal adenomas and carcinomas. PMID: 18957723
  12. Salivary HNP-3concentrations increased following exercise PMID: 19263072
  13. DEFA3 was upregulated in IPF patients with acute exacerbation PMID: 19363140
  14. Studies indicate alpha-defensin, the products of neutrophils wase upregulated at the mRNA and protein levels in SLE patients. PMID: 19758174
  15. Plasma levels of alpha-defensins 1-3 are an indicator of neutrophil activation in pregnant and post-partum women. PMID: 17845323

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Subcellular Location Secreted
Protein Families Alpha-defensin family
Database Links

HGNC: 2762

OMIM: 604522

KEGG: hsa:1668

STRING: 9606.ENSP00000328359

UniGene: Hs.654448

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