Human Oligodendrocyte-myelin glycoprotein(OMG) ELISA kit

Code CSB-EL016334HU
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
oligodendrocyte myelin glycoprotein
Alternative Names
Oligodendrocyte-myelin glycoprotein ELISA Kit; Omg ELISA Kit; OMGP ELISA Kit; OMGP_HUMAN ELISA Kit
Abbreviation
OMG
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human OMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-89  
1:2 Average % 97  
Range % 92-102  
1:4 Average % 92  
Range % 87-98  
1:8 Average % 98  
Range % 94-102  
Recovery
The recovery of human OMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 84-92  
EDTA plasma (n=4) 107 102-112  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.645 2.534 2.590 2.452  
5 1.987 2.096 2.042 1.904  
2.5 1.238 1.345 1.292 1.154  
1.25 0.754 0.765 0.760 0.622  
0.625 0.429 0.446 0.438 0.300  
0.312 0.312 0.324 0.318 0.180  
0.156 0.221 0.226 0.224 0.086  
0 0.137 0.138 0.138    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human OMG ELISA Kit was designed for the quantitative measurement of Human OMG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Citations

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Target Background

Function
(From Uniprot)
Cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system.
Gene References into Functions
  1. The results do not support a relationship between the OMGP gene and the learning disability phenotype observed in neurofibromatosis type 1. PMID: 21534343
  2. novel mutations and novel polymorphisms in coding regions and 5'UTR were detected in patients with with non-syndromic mental retardation PMID: 16425041
  3. Data show that oligodendrocyte-myelin glycoprotein is downregulated in schizophrenia anterior temporal lobe revealed by shotgun proteome analysis. PMID: 19034380
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity
Oligodendrocytes and myelin of the central nervous system.
Database Links

HGNC: 8135

OMIM: 164345

KEGG: hsa:4974

STRING: 9606.ENSP00000247271

UniGene: Hs.113874

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