Human Pantetheinase(VNN1) ELISA kit

Instructions
Code CSB-EL025883HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name vanin 1
Alternative Names HDLCQ8 ELISA Kit; High density lipoprotein cholesterol level quantitative trait locus 8, included ELISA Kit; Pantetheinase ELISA Kit; Pantetheine hydrolase ELISA Kit; Tiff66 ELISA Kit; V-1 ELISA Kit; Vanin 1 ELISA Kit; Vanin-1 ELISA Kit; Vannin 1 ELISA Kit; Vascular non inflammatory molecule 1 ELISA Kit; Vascular non-inflammatory molecule 1 ELISA Kit; VNN 1 ELISA Kit; VNN1 ELISA Kit; VNN1_HUMAN ELISA Kit
Abbreviation VNN1
Uniprot No. O95497
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human pantetheinase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-104  
1:2 Average % 84  
Range % 80-88  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 100  
Range % 95-104  
Recovery
The recovery of human pantetheinase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 80-92  
EDTA plasma (n=4) 94 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
5000 1.984 2.117 2.051 1.949  
2500 1.402 1.326 1.364 1.262  
1250 0.763 0.802 0.783 0.681  
625 0.446 0.475 0.461 0.359  
312.5 0.275 0.284 0.280 0.178  
156.2 0.201 0.193 0.197 0.095  
78 0.164 0.157 0.161 0.059  
0 0.106 0.098 0.102    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
Gene References into Functions
  1. findings suggest that the metabolic pathway catalyzed by VNN1 pantetheinase plays a suppressive role in IAV infection in the respiratory tract, especially in severe conditions under hypercytokinemia PMID: 28576495
  2. Data show that G protein-coupled receptor, family C, group 5, member A protein (GPRC5A) regulates oxidative stress through vanin 1 protein (VNN1). PMID: 28316092
  3. These data suggest that urinary vanin-1 is an early predictive biomarker for decline in eGFR in patients with urothelial carcinoma after dosing of cisplatin PMID: 27317936
  4. serum pantetheinas, encoded by the VNN1 gene, level regulates erythrocyte life span and modulates the risk of developing complicated malaria. PMID: 26343328
  5. VNN1 contributes to corticosteroid responsiveness, and changes in VNN1 nasal epithelial mRNA expression and VNN1 promoter methylation might be clinically useful biomarkers of treatment response in asthmatic children. PMID: 25910714
  6. Our study provides strong biological evidence for the association of the identified SNP with BP and suggests that vanin-1 misfolding and degradation are the underlying molecular mechanism. PMID: 25233454
  7. show that hepatic vanin-1 is under extremely sensitive regulation by PPARalpha and that plasma vanin activity could serve as a readout of changes in PPARalpha activity in human subjects PMID: 24751833
  8. The X-ray crystal structure of human vanin 1 at 2.25 A resolution is presented, which is the first reported structure from the vanin family, as well as a crystal structure of vanin 1 bound to a specific inhibitor. PMID: 25478849
  9. Serum vanin-1 levels may be low in the end-stage renal failure and transiently increase after transplant owing to transient renal function deterioration, which does not lead to elevation of serum creatinine levels in renal transplant patients. PMID: 24702142
  10. Our results suggest that vanin-1 can distinguish between chronic responders and non-responders ITP patients as well as between newly diagnosed ITP patients and healthy controls. PMID: 23534352
  11. Alternative Splicing in VNN1 gene is associated with colorectal cancer. PMID: 24687856
  12. Single-nucleotide polymorphisms in the Regulatory Regions of the VNN1 Gene Are Associated with inflammatory bowel diseases. PMID: 23949622
  13. Microparticle internalization required the interaction of the ectoenzyme Vanin-1 (VNN1), an abundant surface protein on the microparticles, with lipid raft domains of endothelial cells. PMID: 24106341
  14. It isthe enzyme that recycles pantothenic acid (vitamin B5) generated during coenzyme A breakdown and actively involved in the progression of inflammatory reactions by generating cysteamine. PMID: 23978960
  15. Liver contributes to Vanin-1 secretion in serum and PPARalpha is a limiting factor in serum Vnn1 production. PMID: 24140347
  16. data do not support a major role for the Vanin 1 T26I variant in determining blood pressure level and incident ischemic events PMID: 21550219
  17. Our data suggest that VNN1 gene expression and the G-137T variant are associated with HDL-C levels in Mexican children, particularly in prepubertal girls. PMID: 23185446
  18. Urinary vanin-1, an ectoenzyme pantetheinase, distinguished diabetic patients with macroalbuminuria from those with normal albuminuria. PMID: 21544065
  19. Vanin-1 is a specific and sensitive biomarker for renal tubular injury induced by organic solvents. PMID: 21907259
  20. Overexpression of VNN1, an oxidative stress sensor in epithelial cells, was most strongly associated with progression to chronic immune thrombocytopenia PMID: 21325602
  21. The combination of VNN1 and MMP9 may be used as a novel blood biomarker panel for the discrimination of pancreatic cancer-associated diabetes from type 2 diabetes. PMID: 20571492
  22. Pantetheinase (Vnn1) is critical for the host susceptibility to malaria. PMID: 17312006
  23. missense SNP rs2272996 (or N131S) in the VNN1 gene was significantly associated with hypertension in African Americans and the association was replicated in Mexican Americans; a non-significant opposite association was observed in European Americans PMID: 18043751
  24. vanin-1 and vanin-3 are induced at the mRNA and protein level by psoriasis-associated proinflammatory cytokines (Th17/Th1) but not by Th2 cytokines. PMID: 19322213

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Carbon-nitrogen hydrolase superfamily, BTD/VNN family
Tissue Specificity Widely expressed with higher expression in spleen, kidney and blood. Overexpressed in lesional psoriatic skin.
Database Links

HGNC: 12705

OMIM: 603570

KEGG: hsa:8876

STRING: 9606.ENSP00000356905

UniGene: Hs.12114

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