Human Paraoxonase 3 (PON3) ELISA Kit

Code CSB-E15783h
Size 96T,5×96T,10×96T
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Product Details

Target Name
paraoxonase 3
Alternative Names
2810004E20 ELISA Kit; AI786302 ELISA Kit; MGC95026 ELISA Kit; Paraoxonase 3 ELISA Kit; Pon3 ELISA Kit; PON3_HUMAN ELISA Kit; Serum paraoxonase/lactonase 3 ELISA Kit
Abbreviation
PON3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PON3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 96  
Range % 90-106  
1:2000 Average % 91  
Range % 82-99  
1:4000 Average % 89  
Range % 80-97  
1:8000 Average % 93  
Range % 85-102  
Recovery
The recovery of human PON3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 85-105  
EDTA plasma (n=4) 95 88-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.472 2.411 2.442 2.356  
5 1.873 1.860 1.867 1.781  
2.5 1.264 1.243 1.254 1.168  
1.25 0.721 0.714 0.718 0.632  
0.625 0.542 0.568 0.555 0.469  
0.312 0.282 0.296 0.289 0.203  
0.156 0.206 0.217 0.212 0.126  
0 0.085 0.086 0.086    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PON3 ELISA Kit was designed for the quantitative measurement of Human PON3 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
Gene References into Functions
  1. PON3 suppressed cell proliferation in vivo and in vitro, which was attributed to its cell-cycle arrest effect. PMID: 27661119
  2. PON3 protein can be detected in plasma and resides in the high-density lipoprotein fraction and protects against Oxidative stress by hydrolyzing certain oxidized lipids in lipoproteins, macrophages and Atherosclerotic lesions. PMID: 29308836
  3. Although inappropriate promoter methylation was not invariantly associated with reduced transcript expression, a significant association was apparent for the ARHGEF4, PON3, STAT5a, and VAX2 gene transcripts (P<0.05). Herein, we present the first genome-wide DNA methylation analysis in a unique HG-NMIBC cohort, showing extensive and discrete methylation changes relative to normal bladder and low-intermediate-grade tumor PMID: 26929985
  4. Low PON3 expression is associated with hepatocellular carcinoma progression. PMID: 27553024
  5. This study showed that the PI3K/Akt pathway is up-regulated by the expression of PON3 in oral squamous cell carcinoma by AP-1. PMID: 27923688
  6. PON3 SNP rs13226149 was associated with intracerebral hemorrhage in log-additive and dominant models. The A allele of rs13226149 contributed to the decreased risk of ICH. SNP rs1053275 was not associated with ICH. PMID: 26227792
  7. Interaction of PON-3 and PON-1 and HDL are associated with the presence and severity of coronary artery disease in type 2 diabetes mellitus patients. PMID: 25964115
  8. PON3 in HDL may be an important protein in preventing atherosclerosis. PMID: 25723336
  9. PON3 is upregulated in cancer tissues and protects against mitochondrial superoxide-mediated cell death. PMID: 22441669
  10. Our results suggest that low serum paraoxonase activity is a risk factor for Alzheimer disease. Furthermore, multiple variants in PON influence serum paraoxonase activity and their effects may be synergistic. PMID: 20980077
  11. Increased serum paraoxonase-3 concentration is associated with insulin sensitivity in peripheral artery disease and with inflammation in coronary artery disease. PMID: 22153698
  12. This study reports for the first time an important increase in serum PON3 concentrations in HIV-infected patients that is associated with their oxidative status and their treatment with NNRTI. PMID: 22003209
  13. investigation of whether gene silencing of Pon3 causes oxidative stress in cell line: Data suggest that PON3 knockdown reduces cell proliferation and total antioxidant capacity at ambient oxygen levels. PMID: 21952037
  14. Serum PON3 concentrations showed a moderate influence (about 10% variation) by PON3 promoter polymorphisms. PMID: 21335322
  15. Paraoxonase-3, a putative circulating antioxidant, is systemically up-regulated in late gestation in the fetal rat, sheep, and human PMID: 20463093
  16. Decreased coronary heart disease risk associated with polymorphisms of paraoxonase 1. PMID: 12151850
  17. Organophosphates are hydrolyzed almost exclusively by PON1, lovastatin and spironolactone are hydrolyzed only by PON3. All three proteins hydrolyze and thereby inactivate N-acyl-homoserine lactones, which are quorum-sensing signals of pathogenic bacteria. PMID: 15772423
  18. The refolded recombinant PON3 exhibited similar antioxidant activity to that of PON3 purified from the soluble fraction of cell lysate and could effectively protect LDL from Cu2+ induced oxidation. PMID: 16139510
  19. Elevated human paraoxanase 3 protein expression significantly decreases atherosclerotic lesion formation and adiposity in male transgenic mice. PMID: 17379834
  20. The rates of hydrolysis of estrogen esters by the paraoxonases is PON3 > PON1 > PON2; diesters are better substrates for the PONs and are very efficiently hydrolyzed, particularly by PON3. PMID: 17412306
  21. Report protective effects of transgene expressed human PON3 against CCl4-induced subacute liver injury in mice. PMID: 19345057
  22. The directed evolution and characterization of recombinant variants of serum paraoxonase PON3, is described. PMID: 19492856

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Subcellular Location
Secreted, extracellular space.
Protein Families
Paraoxonase family
Database Links

HGNC: 9206

OMIM: 602720

KEGG: hsa:5446

STRING: 9606.ENSP00000265627

UniGene: Hs.440967

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