Recombinant Human Serum paraoxonase/lactonase 3 (PON3)

Code CSB-YP614883HU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP614883HU
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP614883HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP614883HU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP614883HU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
PON3
Uniprot No.
Alternative Names
2810004E20; AI786302; MGC95026; Paraoxonase 3; Pon3; PON3_HUMAN; Serum paraoxonase/lactonase 3
Species
Homo sapiens (Human)
Expression Region
2-354
Target Protein Sequence
GKLVALVLL GVGLSLVGEM FLAFRERVNA SREVEPVEPE NCHLIEELES GSEDIDILPS GLAFISSGLK YPGMPNFAPD EPGKIFLMDL NEQNPRAQAL EISGGFDKEL FNPHGISIFI DKDNTVYLYV VNHPHMKSTV EIFKFEEQQR SLVYLKTIKH ELLKSVNDIV VLGPEQFYAT RDHYFTNSLL SFFEMILDLR WTYVLFYSPR EVKVVAKGFC SANGITVSAD QKYVYVADVA AKNIHIMEKH DNWDLTQLKV IQLGTLVDNL TVDPATGDIL AGCHPNPMKL LNYNPEDPPG SEVLRIQNVL SEKPRVSTVY ANNGSVLQGT SVASVYHGKI LIGTVFHKTL YCEL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
Gene References into Functions
  1. PON3 suppressed cell proliferation in vivo and in vitro, which was attributed to its cell-cycle arrest effect. PMID: 27661119
  2. PON3 protein can be detected in plasma and resides in the high-density lipoprotein fraction and protects against Oxidative stress by hydrolyzing certain oxidized lipids in lipoproteins, macrophages and Atherosclerotic lesions. PMID: 29308836
  3. Although inappropriate promoter methylation was not invariantly associated with reduced transcript expression, a significant association was apparent for the ARHGEF4, PON3, STAT5a, and VAX2 gene transcripts (P<0.05). Herein, we present the first genome-wide DNA methylation analysis in a unique HG-NMIBC cohort, showing extensive and discrete methylation changes relative to normal bladder and low-intermediate-grade tumor PMID: 26929985
  4. Low PON3 expression is associated with hepatocellular carcinoma progression. PMID: 27553024
  5. This study showed that the PI3K/Akt pathway is up-regulated by the expression of PON3 in oral squamous cell carcinoma by AP-1. PMID: 27923688
  6. PON3 SNP rs13226149 was associated with intracerebral hemorrhage in log-additive and dominant models. The A allele of rs13226149 contributed to the decreased risk of ICH. SNP rs1053275 was not associated with ICH. PMID: 26227792
  7. Interaction of PON-3 and PON-1 and HDL are associated with the presence and severity of coronary artery disease in type 2 diabetes mellitus patients. PMID: 25964115
  8. PON3 in HDL may be an important protein in preventing atherosclerosis. PMID: 25723336
  9. PON3 is upregulated in cancer tissues and protects against mitochondrial superoxide-mediated cell death. PMID: 22441669
  10. Our results suggest that low serum paraoxonase activity is a risk factor for Alzheimer disease. Furthermore, multiple variants in PON influence serum paraoxonase activity and their effects may be synergistic. PMID: 20980077
  11. Increased serum paraoxonase-3 concentration is associated with insulin sensitivity in peripheral artery disease and with inflammation in coronary artery disease. PMID: 22153698
  12. This study reports for the first time an important increase in serum PON3 concentrations in HIV-infected patients that is associated with their oxidative status and their treatment with NNRTI. PMID: 22003209
  13. investigation of whether gene silencing of Pon3 causes oxidative stress in cell line: Data suggest that PON3 knockdown reduces cell proliferation and total antioxidant capacity at ambient oxygen levels. PMID: 21952037
  14. Serum PON3 concentrations showed a moderate influence (about 10% variation) by PON3 promoter polymorphisms. PMID: 21335322
  15. Paraoxonase-3, a putative circulating antioxidant, is systemically up-regulated in late gestation in the fetal rat, sheep, and human PMID: 20463093
  16. Decreased coronary heart disease risk associated with polymorphisms of paraoxonase 1. PMID: 12151850
  17. Organophosphates are hydrolyzed almost exclusively by PON1, lovastatin and spironolactone are hydrolyzed only by PON3. All three proteins hydrolyze and thereby inactivate N-acyl-homoserine lactones, which are quorum-sensing signals of pathogenic bacteria. PMID: 15772423
  18. The refolded recombinant PON3 exhibited similar antioxidant activity to that of PON3 purified from the soluble fraction of cell lysate and could effectively protect LDL from Cu2+ induced oxidation. PMID: 16139510
  19. Elevated human paraoxanase 3 protein expression significantly decreases atherosclerotic lesion formation and adiposity in male transgenic mice. PMID: 17379834
  20. The rates of hydrolysis of estrogen esters by the paraoxonases is PON3 > PON1 > PON2; diesters are better substrates for the PONs and are very efficiently hydrolyzed, particularly by PON3. PMID: 17412306
  21. Report protective effects of transgene expressed human PON3 against CCl4-induced subacute liver injury in mice. PMID: 19345057
  22. The directed evolution and characterization of recombinant variants of serum paraoxonase PON3, is described. PMID: 19492856

Show More

Hide All

Subcellular Location
Secreted, extracellular space.
Protein Families
Paraoxonase family
Database Links

HGNC: 9206

OMIM: 602720

KEGG: hsa:5446

STRING: 9606.ENSP00000265627

UniGene: Hs.440967

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1