Product Details

Target Name

phospholipase A1 member A

Alternative Names

AA986889 ELISA Kit; NMD ELISA Kit; OTTHUMP00000215248 ELISA Kit; OTTHUMP00000215249 ELISA Kit; OTTHUMP00000215250 ELISA Kit; Phosphatidylserine specific phospholipase A1 ELISA Kit; Phosphatidylserine specific phospholipase A1alpha ELISA Kit; Phosphatidylserine-specific phospholipase A1 ELISA Kit; Phospholipase A1 member A ELISA Kit; pla1a ELISA Kit; PLA1A_HUMAN ELISA Kit; PS PLA1 ELISA Kit; PS-PLA1 ELISA Kit; PSPLA 1 ELISA Kit; PSPLA1 ELISA Kit

Abbreviation

PLA1A

Uniprot No.

Q53H76

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PLA1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-95
1:2	Average %	97
	Range %	93-101
1:4	Average %	99
	Range %	95-106
1:8	Average %	93
	Range %	87-99

Recovery

The recovery of human PLA1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	99	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.340	2.344	2.342	2.237
10	1.604	1.741	1.673	1.568
5	0.936	0.968	0.952	0.847
2.5	0.576	0.554	0.565	0.460
1.25	0.353	0.325	0.339	0.234
0.625	0.230	0.213	0.222	0.117
0.312	0.185	0.180	0.183	0.078
0	0.104	0.106	0.105

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Target Background

Function
(From Uniprot)

Hydrolyzes the ester bond of the acyl group attached at the sn-1 position of phosphatidylserines (phospholipase A1 activity) and 1-acyl-2-lysophosphatidylserines (lysophospholipase activity) in the pathway of phosphatidylserines acyl chain remodeling. Cleaves phosphatidylserines exposed on the outer leaflet of the plasma membrane of apoptotic cells producing 2-acyl-1-lysophosphatidylserines, which in turn enhance mast cell activation and histamine production. Has no activity toward other glycerophospholipids including phosphatidylcholines, phosphatidylethanolamines, phosphatidic acids or phosphatidylinositols, or glycerolipids such as triolein.; Hydrolyzes lyso-PS but not PS.

Gene References into Functions

Serum PS-PLA1 levels were significantly higher in the melanoma subjects and associated with the clinical stages. PMID: 29500864
the LysoPA levels might be determined largely by LysoPC and LysoPI (possible precursors) and the PS-PLA1-mediated pathway might be involved in the production of LysoPS in gastric cancer. PMID: 28143894
PS-PLA1 expression in colorectal cancer is associated with tumor invasion and metastasis. PMID: 25750298
These data suggest that PLA1A plays an important role in bridging the membrane-associated NS2-E2 complex and the NS5A-associated replication complex via its interaction with hepatitis C virus E2, NS2, and NS5A. PMID: 25505071
PLA1A2 polymorphism is associated with mortality in participants whose hemoglobin A1c ranges from 5.5% to 6.5%. PMID: 24886443
Overall, our study shed the light on new structural features of the phospholipase activity of pancreatic lipase family members. PMID: 24368210
These results suggest that the expression of PS-PLA(1) mRNA in THP-1-derived macrophages is activated via TLR4. PMID: 20573295
polymorphisms in the gene encoding phosphatidylserine-specific phospholipase A1 PMID: 12436198

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Subcellular Location

Secreted.

Protein Families

AB hydrolase superfamily, Lipase family

Tissue Specificity

Widely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney.

Database Links

HGNC: 17661

OMIM: 607460

KEGG: hsa:51365

STRING: 9606.ENSP00000273371

UniGene: Hs.437451

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Human Phospholipase A1 member A(PLA1A) ELISA kit

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Target Background