PLA1A Antibody

Datasheet

Code	CSB-PA018084LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: PLA1A antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 41, 48, 32 kDa Observed band size: 50 kDa IHC image of CSB-PA018084LA01HU diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA018084LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PLA1A Polyclonal antibody

Uniprot No.

Q53H76

Target Names

PLA1A

Alternative Names

AA986889 antibody; NMD antibody; OTTHUMP00000215248 antibody; OTTHUMP00000215249 antibody; OTTHUMP00000215250 antibody; Phosphatidylserine specific phospholipase A1 antibody; Phosphatidylserine specific phospholipase A1alpha antibody; Phosphatidylserine-specific phospholipase A1 antibody; Phospholipase A1 member A antibody; pla1a antibody; PLA1A_HUMAN antibody; PS PLA1 antibody; PS-PLA1 antibody; PSPLA 1 antibody; PSPLA1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Phospholipase A1 member A protein (293-398AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PLA1A Antibody (Product code: CSB-PA018084LA01HU) is Non-conjugated. For PLA1A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018084LB01HU	PLA1A Antibody, HRP conjugated	ELISA
FITC	CSB-PA018084LC01HU	PLA1A Antibody, FITC conjugated
Biotin	CSB-PA018084LD01HU	PLA1A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Hydrolyzes the ester bond of the acyl group attached at the sn-1 position of phosphatidylserines (phospholipase A1 activity) and 1-acyl-2-lysophosphatidylserines (lysophospholipase activity) in the pathway of phosphatidylserines acyl chain remodeling. Cleaves phosphatidylserines exposed on the outer leaflet of the plasma membrane of apoptotic cells producing 2-acyl-1-lysophosphatidylserines, which in turn enhance mast cell activation and histamine production. Has no activity toward other glycerophospholipids including phosphatidylcholines, phosphatidylethanolamines, phosphatidic acids or phosphatidylinositols, or glycerolipids such as triolein.; Hydrolyzes lyso-PS but not PS.

Gene References into Functions

Serum PS-PLA1 levels were significantly higher in the melanoma subjects and associated with the clinical stages. PMID: 29500864
the LysoPA levels might be determined largely by LysoPC and LysoPI (possible precursors) and the PS-PLA1-mediated pathway might be involved in the production of LysoPS in gastric cancer. PMID: 28143894
PS-PLA1 expression in colorectal cancer is associated with tumor invasion and metastasis. PMID: 25750298
These data suggest that PLA1A plays an important role in bridging the membrane-associated NS2-E2 complex and the NS5A-associated replication complex via its interaction with hepatitis C virus E2, NS2, and NS5A. PMID: 25505071
PLA1A2 polymorphism is associated with mortality in participants whose hemoglobin A1c ranges from 5.5% to 6.5%. PMID: 24886443
Overall, our study shed the light on new structural features of the phospholipase activity of pancreatic lipase family members. PMID: 24368210
These results suggest that the expression of PS-PLA(1) mRNA in THP-1-derived macrophages is activated via TLR4. PMID: 20573295
polymorphisms in the gene encoding phosphatidylserine-specific phospholipase A1 PMID: 12436198

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Subcellular Location

Secreted.

Protein Families

AB hydrolase superfamily, Lipase family

Tissue Specificity

Widely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney.

Database Links

HGNC: 17661

OMIM: 607460

KEGG: hsa:51365

STRING: 9606.ENSP00000273371

UniGene: Hs.437451

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Ras signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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