Human Platelet receptor Gi24(C10orf54) ELISA kit

Code CSB-EL002961HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
chromosome 10 open reading frame 54
Alternative Names
B7 H5 ELISA Kit; B7H5 ELISA Kit; C10orf54 ELISA Kit; chromosome 10 open reading frame 54 ELISA Kit; DD1alpha ELISA Kit; GI24 ELISA Kit; GI24_HUMAN ELISA Kit; PD-1H ELISA Kit; PDCD1 homolog ELISA Kit; Platelet receptor Gi24 ELISA Kit; PP2135 ELISA Kit; sisp 1 ELISA Kit; SISP1 ELISA Kit; stress induced secreted protein 1 ELISA Kit; UNQ730/PRO1412 ELISA Kit; V domain Ig suppressor of T cell activation ELISA Kit; V set domain containing immunoregulatory receptor ELISA Kit; V set immunoregulatory receptor ELISA Kit; V type immunoglobulin domain containing suppressor of T cell activation ELISA Kit; VISTA ELISA Kit
Abbreviation
C10orf54
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C10orf54 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 83-89
1:2 Average % 100
Range % 95-105
1:4 Average % 84
Range % 81-89
1:8 Average % 92
Range % 88-96
Recovery
The recovery of human C10orf54 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 98-104
EDTA plasma (n=4) 99 95-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.366 2.305 2.336 2.213
5 1.642 1.658 1.650 1.527
2.5 0.898 0.868 0.883 0.760
1.25 0.475 0.455 0.465 0.342
0.625 0.320 0.326 0.323 0.200
0.312 0.249 0.251 0.250 0.127
0.156 0.195 0.193 0.194 0.071
0 0.125 0.120 0.123  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human C10orf54 ELISA Kit was designed for the quantitative measurement of Human C10orf54 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.
Gene References into Functions
  1. Data indicate that VISTA is predominantly expressed and up-regulated in the high-density infiltrated immune cells but minimal in pancreatic cancerous cells. PMID: 29771768
  2. High VISTA expression is associated with colorectal carcinoma. PMID: 30128738
  3. VISTA expression supports immune-complex inflammation in collagen antibody-induced arthritis and VISTA is expressed in human synovium PMID: 29216931
  4. This study is the first to describe the expression of VISTA-expressing lymphocytes in melanoma samples and in the context of acquired resistance to immune checkpoint inhibitors PMID: 28776578
  5. Taken together, the results indicated that the VISTA high and CD8 low group, as an immunosuppressive subgroup, might be associated with a poor prognosis in primary OSCC. These findings indicated that VISTA might be a potential immunotherapeutic target in OSCC treatment. PMID: 28236118
  6. this review describes the functions of VISTA in the context of cancer immunotherapy PMID: 28258694
  7. overexpression of the newly described co-stimulatory molecule, PD1 homologue (PD-1H) in human monocyte/macrophages is sufficient to induce spontaneous secretion of multiple cytokines. PMID: 25279955
  8. p53-induced expression of DD1alpha prevents persistence of cell corpses and ensures efficient generation of precise immune responses in mice. PMID: 26228159
  9. Results suggest that GI24 contributes to tumor-invasive growth in the collagen matrix by augmenting cell surface MT1-MMP. PMID: 20666777

Show More

Hide All

Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta
Database Links

HGNC: 30085

OMIM: 615608

KEGG: hsa:64115

STRING: 9606.ENSP00000378409

UniGene: Hs.47382

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X