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VSIR Recombinant Monoclonal Antibody

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Datasheet
Code CSB-RA884484MA1HU
Size $49.9
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  • The Binding Activity of Human VSIR with Anti-VSIR Recombinant antibody
    Activity: Measured by its binding ability in a functional ELISA. Immobilized Human VSIR (CSB-MP884484HU) at 2 μg/mL can bind Anti-VSIR recombinant antibody. The EC50 is 20.84-22.73 ng/mL.
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Product Details

Uniprot No.
Q9H7M9
Target Names
VSIR
Alternative Names
B7 H5 antibody; B7H5 antibody; C10orf54 antibody; chromosome 10 open reading frame 54 antibody; DD1alpha antibody; GI24 antibody; GI24_HUMAN antibody; PD-1H antibody; PDCD1 homolog antibody; Platelet receptor Gi24 antibody; PP2135 antibody; sisp 1 antibody; SISP1 antibody; stress induced secreted protein 1 antibody; UNQ730/PRO1412 antibody; V domain Ig suppressor of T cell activation antibody; V set domain containing immunoregulatory receptor antibody; V set immunoregulatory receptor antibody; V type immunoglobulin domain containing suppressor of T cell activation antibody; VISTA antibody
Species Reactivity
Human
Immunogen
Recombinant Human VSIR protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
15F4
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA
Recommended Dilution
Application Recommended Dilution
ELISA 1:5000-1:50000
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

VSIR, also known as VISTA (V-domain Ig Suppressor of T cell Activation), represents a critical immune checkpoint regulator that has emerged as a significant target in immunology and oncology research. This inhibitory receptor, expressed predominantly on myeloid cells and T lymphocytes, plays a central role in suppressing T cell activation and maintaining immune homeostasis. Understanding VSIR biology has become increasingly important for researchers investigating immune regulation, tumor microenvironment dynamics, and potential therapeutic interventions targeting checkpoint pathways.

This recombinant monoclonal antibody, clone 15F4, offers the consistency and reliability that demanding experimental workflows require. Because it is produced using recombinant technology with a defined sequence, you can expect exceptional lot-to-lot reproducibility throughout your research program. The human IgG1 isotype format and affinity-chromatography purification ensure high purity and specificity for detecting human VSIR.

Functional validation demonstrates robust binding activity, with the antibody showing strong interaction with immobilized human VSIR protein in ELISA format. The EC50 values of 20.84–22.73 ng/mL reflect excellent sensitivity, allowing you to work across a broad dilution range of 1:5000 to 1:50000 depending on your specific assay conditions. This flexibility enables optimization for various experimental setups while maintaining reliable detection.

The unconjugated format provides versatility for pairing with your preferred secondary detection systems. Supplied in a glycerol-based PBS buffer with Proclin 300 preservative, the antibody maintains stability when stored at -20°C or -80°C. For researchers exploring immune checkpoint biology, T cell regulation, or cardiovascular-related immune mechanisms, this antibody delivers the reproducible performance essential for generating publication-quality data.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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VSIR Antibodies

VSIR Proteins

VSIR ELISA kits

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Target Background

Function
Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.
Gene References into Functions
  1. Data indicate that VISTA is predominantly expressed and up-regulated in the high-density infiltrated immune cells but minimal in pancreatic cancerous cells. PMID: 29771768
  2. High VISTA expression is associated with colorectal carcinoma. PMID: 30128738
  3. VISTA expression supports immune-complex inflammation in collagen antibody-induced arthritis and VISTA is expressed in human synovium PMID: 29216931
  4. This study is the first to describe the expression of VISTA-expressing lymphocytes in melanoma samples and in the context of acquired resistance to immune checkpoint inhibitors PMID: 28776578
  5. Taken together, the results indicated that the VISTA high and CD8 low group, as an immunosuppressive subgroup, might be associated with a poor prognosis in primary OSCC. These findings indicated that VISTA might be a potential immunotherapeutic target in OSCC treatment. PMID: 28236118
  6. this review describes the functions of VISTA in the context of cancer immunotherapy PMID: 28258694
  7. overexpression of the newly described co-stimulatory molecule, PD1 homologue (PD-1H) in human monocyte/macrophages is sufficient to induce spontaneous secretion of multiple cytokines. PMID: 25279955
  8. p53-induced expression of DD1alpha prevents persistence of cell corpses and ensures efficient generation of precise immune responses in mice. PMID: 26228159
  9. Results suggest that GI24 contributes to tumor-invasive growth in the collagen matrix by augmenting cell surface MT1-MMP. PMID: 20666777

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta
Database Links

HGNC: 30085

OMIM: 615608

KEGG: hsa:64115

STRING: 9606.ENSP00000378409

UniGene: Hs.47382

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