Human Protein S100-A7A(S100A7A) ELISA kit

Code CSB-EL020636HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
S100 calcium binding protein A7A
Alternative Names
S100A7A ELISA Kit; S100A15 ELISA Kit; S100A7L1 ELISA Kit; Protein S100-A7A ELISA Kit; S100 calcium-binding protein A15 ELISA Kit; S100 calcium-binding protein A7-like 1 ELISA Kit; S100 calcium-binding protein A7A ELISA Kit
Abbreviation
S100A7A
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, urine
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human S100A7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-97
1:2 Average % 92
Range % 89-96
1:4 Average % 98
Range % 94-102
1:8 Average % 91
Range % 82-97
Recovery
The recovery of human S100A7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-101
EDTA plasma (n=4) 86 81-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.834 2.912 2.873 2.758
10 2.175 2.017 2.096 1.981
5 1.286 1.218 1.252 1.137
2.5 0.654 0.664 0.659 0.544
1.25 0.397 0.386 0.392 0.277
0.625 0.249 0.242 0.246 0.131
0.312 0.167 0.162 0.165 0.050
0 0.115 0.114 0.115  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human S100A7A ELISA Kit was designed for the quantitative measurement of Human S100A7A protein in serum, plasma, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Function
(From Uniprot)
May be involved in epidermal differentiation and inflammation and might therefore be important for the pathogenesis of psoriasis and other diseases.
Gene References into Functions
  1. S100A15 retains the ability to coordinate zinc through incorporation of an exogenous ligand resulting in a unique zinc-binding site among S100 proteins. PMID: 22747601
  2. Molecular cloning and characterization of alternatively spliced S100A15 isoforms from psoriatic skin. PMID: 12923069
  3. The S100A15 protein has been expressed & purified to homogeneity in 2 crystal forms. Crystals of form I are triclinic. Crystals of form II are monoclinic & have a solvent content of 51.8%, corresponding to the presence of 1 monomer in the asymmetric unit. PMID: 16682778
  4. Overexpression of hS100A15 in chronic inflammatory skin diseases and regulation by inflammatory cytokines and calcium suggest that hS100A15 is involved in Th1-associated epithelial responses and epidermal maturation in normal and diseased human skin PMID: 17620096
  5. E. coli modulates S100A15 and IL-8 expression of keratinocytes by recognition through TLR4. PMID: 17625598
  6. study defines RAGE (receptor for advanced glycation end products) as the hS100A7 receptor, whereas hS100A15 functions through a Gi protein-coupled receptor PMID: 18606705

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Subcellular Location
Cytoplasm.
Protein Families
S-100 family
Tissue Specificity
Overexpressed in psoriasis.
Database Links

HGNC: 21657

OMIM: 617427

KEGG: hsa:338324

UniGene: Hs.442337

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