Human Protein Wnt-2b(WNT2B) ELISA kit

Instructions
Code CSB-EL026134HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name wingless-type MMTV integration site family, member 2B
Alternative Names Protein Wnt-13 ELISA Kit; Protein Wnt-2b ELISA Kit; Wingless type MMTV integration site family, member 13 ELISA Kit; Wingless type MMTV integration site family, member 2B ELISA Kit; WNT13 ELISA Kit; Wnt2b ELISA Kit; WNT2B_HUMAN ELISA Kit; XWNT2 ELISA Kit; XWNT2, Xenopus, homolog of ELISA Kit
Abbreviation WNT2B
Uniprot No. Q93097
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human WNT2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 83-94
1:2 Average % 98
Range % 94-101
1:4 Average % 102
Range % 98-105
1:8 Average % 95
Range % 90-101
Recovery
The recovery of human WNT2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 85-105
EDTA plasma (n=4) 104 88-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.513 2.355 2.434 2.285
2000 2.012 1.980 1.996 1.847
1000 1.220 1.222 1.221 1.072
500 0.725 0.721 0.723 0.574
250 0.417 0.421 0.419 0.270
125 0.298 0.282 0.290 0.141
62.5 0.227 0.224 0.226 0.077
0 0.148 0.150 0.149  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
Gene References into Functions
  1. Wnt2B co-operates with Frizzled7 to mediate mesenchymal to epithelial transition in colorectal cancer. PMID: 28560804
  2. Altered WNT2B expression in bladder wall fibroblasts alters expression of the apoptosis inductor TNFSF10. PMID: 29131138
  3. we identified two independent SNPs (i.e., WNT2B rs1175649 G>T and BTRC rs61873997 G>A) that showed a predictive role in CM-specific survival, with an effect-allele-attributed hazards ratio (adjusted hazards ratio) of 1.99 (95% confidence interval = 1.41-2.81, P = 8.10 x 10(-5)) and 0.61 (0.46-0.80, 3.12x10(-4)), respectively. PMID: 28499756
  4. A549-Luc-positive cells showed cytotoxicity following exposure to the Ad-shWnt2B vector. The percentage of viable cells was significantly lower in A549-Luc cells treated with Ad-shWnt2B than with Ad-scramble (p<0.01 versus control or Ad-scramble, respectively). PMID: 27793913
  5. ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity. The molecular pathway is probably relevant for intestinal homeostasis, overwhelmed by other pathways in carcinogenesis. PMID: 25356045
  6. these results indicate that the high-expression levels of Gli1 and Wnt2B might play a pivotal role during tumorigenesis of pancreatic cancer PMID: 25120849
  7. Results showed that miR-185-3p contributs to radioresistance of nasopharyngeal carcinoma via modulation of WNT2B expression. PMID: 25297925
  8. secretion of WNT2B and WNT9B and stabilization of beta-catenin (CTNNB1) upon virus infection negatively regulate expression of representative inducible genes IFNB1, IFIT1 and TNF in a CTNNB1-dependent effector mechanism PMID: 23785285
  9. High Wnt2B overexpression is associated with ovarian cancer metastasis and drug resistance. PMID: 22635028
  10. This is the first report on differential regulation of WNT2 and WNT2B mRNAs in human cancer cell lines. PMID: 11712082
  11. Wnt-2b mRNA is expressed in human ovarian cancer cells PMID: 12072409
  12. REVIEW: Anti-WNT2B monoclonal antibodies, WNT2B RNAi compounds, or small molecule WNT2B inhibitors could be developed as novel therapeutic agents for gastric cancer and esophageal cancer in the field of clinical oncology. PMID: 16273293
  13. in addition to alternative promoters and RNA splicing, an alternative translation start in Wnt13B and Wnt13C mRNAs increases the complexity of both human wnt13 expression and functions PMID: 16407296
  14. Heplipin induced KG-1 cell apoptosis is related with Wntl3 and ATPase3. PMID: 17649721
  15. Hedgehog signals and bHLH transcription factors are involved in WNT2B upregulation, which is counteracted by BMP, WNT and Notch signals. PMID: 19360354

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Tissue Specificity Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidne
Database Links

HGNC: 12781

OMIM: 601968

KEGG: hsa:7482

STRING: 9606.ENSP00000358698

UniGene: Hs.258575

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