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Human Protein disulfide-isomerase A4(PDIA4) ELISA kit

Protocol
Code CSB-EL017722HU
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
Calcium binding protein intestinal related ELISA Kit; Endoplasmic reticulum resident protein 70 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; EPR70 ELISA Kit; EPR72 ELISA Kit; ER protein 70 ELISA Kit; ER protein 72 ELISA Kit; ERP 70 ELISA Kit; ERp 72 ELISA Kit; ERp-72 ELISA Kit; ERp70 ELISA Kit; ERp72 ELISA Kit; PDIA 4 ELISA Kit; Pdia4 ELISA Kit; PDIA4_HUMAN ELISA Kit; PDIR ELISA Kit; Protein disulfide isomerase A4 ELISA Kit; Protein disulfide isomerase associated 4 ELISA Kit; Protein disulfide isomerase family A member 4 ELISA Kit; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related) ELISA Kit; Protein disulfide isomerase related protein ELISA Kit; Protein disulfide-isomerase A4 ELISA Kit; Protein ERp 72 ELISA Kit; Protein ERp72 ELISA Kit
Abbreviation
PDIA4
Uniprot No.
P13667
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
23.5 pg/ml-1500 pg/ml
Sensitivity
5.8 pg/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 89-97  
1:2 Average % 100  
Range % 96-104  
1:4 Average % 90  
Range % 88-93  
1:8 Average % 91  
Range % 86-94  
Recovery
The recovery of human PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-94  
EDTA plasma (n=4) 104 99-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 1.960 1.977 1.969 1.817  
750 1.284 1.300 1.292 1.140  
375 0.725 0.713 0.719 0.567  
187.5 0.412 0.406 0.409 0.257  
94 0.287 0.277 0.282 0.130  
47 0.220 0.215 0.218 0.066  
23.5 0.184 0.178 0.181 0.029  
0 0.150 0.154 0.152    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Human PDIA4 ELISA Kit was designed for the quantitative measurement of Human PDIA4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

Related Products

PDIA4 Antibodies

PDIA4 Proteins

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Target Background

Gene References into Functions
  1. up-regulated by IL11 in first trimester placenta PMID: 28487027
  2. PDIA4 as a negative regulator of cancer cell apoptosis. PMID: 28534513
  3. Participants with metabolic syndrome (MetS) had significantly higher serum PDIA4 levels than those without MetS (P<0.001). After adjustments, the individuals with the highest PDIA4 tertile were associated with a higher risk of MetS than those with the lowest tertile (OR = 4.83, 95% CI: 2.71-8.60). PMID: 28650993
  4. CDDP-resistant non-small lung cancer cells undergo profound remodeling of their endoplasmic reticulum (ER) proteome (>80 proteins identified by proteomics) and exhibit a dramatic overexpression of two protein disulfide isomerases, PDIA4 and PDIA6. PMID: 24464223
  5. novel esophageal squamous cell carcinoma marker PDIA4 was identified by mass spectrometry and immunohistochemical analysis PMID: 21743296
  6. Nox1 associates with ERp72, which involves its N-terminus encompassing a Ca(2+)-binding site and the first TRX-like motif. PMID: 18620548

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Subcellular Location
Endoplasmic reticulum lumen. Melanosome.
Protein Families
Protein disulfide isomerase family
Database Links

HGNC: 30167

KEGG: hsa:9601

STRING: 9606.ENSP00000286091

UniGene: Hs.93659

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