Human Protein dopey-2(DOPEY2) ELISA kit

Code CSB-EL007119HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
dopey family member 2
Alternative Names
DOP1B ELISA Kit; C21orf5 ELISA Kit; DOPEY2 ELISA Kit; KIAA0933Protein dopey-2 ELISA Kit
Abbreviation
DOPEY2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
32 pg/mL-1200 pg/mL
Sensitivity
8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DOPEY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %86-97
1:2Average %97
Range %92-103
1:4Average %85
Range %81-89
1:8Average %89
Range %83-95
Recovery
The recovery of human DOPEY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 104102-106
EDTA plasma (n=4)9390-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.313 2.451 2.382 2.335
6401.754 1.802 1.778 1.731
3201.012 1.032 1.022 0.975
800.345 0.355 0.350 0.303
320.173 0.174 0.174 0.127
00.045 0.048 0.047
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human DOPEY2 ELISA Kit was designed for the quantitative measurement of Human DOPEY2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 32 pg/mL-1200 pg/mL and the sensitivity is 8 pg/mL.

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Target Background

Function
(From Uniprot)
May play a role in regulating membrane trafficking of cargo proteins. Together with ATP9A and MON2, regulates SNX3 retromer-mediated endosomal sorting of WLS away from lysosomal degradation.
Gene References into Functions
  1. The differentially expressed C21orf5 gene in the medial temporal-lobe system could play a role in Down syndrome. PMID: 12767918
  2. C21orf5 selective expression in the key brain structures for learning and memory suggests that C21orf5 overexpression could participate in mental retardation pathogenesis in Down syndrome patients. PMID: 16276086
  3. C21orf5, could play a role in brain morphogenesis and, when overexpressed, it could participate in neurological features and mental retardation observed in DS patients. PMID: 16303751
  4. Results quantify and statistically analyze, for the first time, DOPEY2 expression variations in different regions of the Down syndrome human fetal brains and to compare them to corresponding normal brains. PMID: 19460634
Subcellular Location
Early endosome membrane. Golgi apparatus membrane; Peripheral membrane protein.
Protein Families
Dopey family
Tissue Specificity
Ubiquitously expressed. Overexpressed in lymphoblasts from Down syndrome patients.
Database Links

HGNC: 1291

OMIM: 604803

KEGG: hsa:9980

STRING: 9606.ENSP00000382104

UniGene: Hs.204575

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