Human Reticulon-4 receptor-like 2(RTN4RL2) ELISA kit

Product Details

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Target Background

Product Details

Target Name

reticulon 4 receptor-like 2

Alternative Names

RTN4RL2 ELISA Kit; NGRH1 ELISA Kit; NGRL3 ELISA Kit; Reticulon-4 receptor-like 2 ELISA Kit; Nogo receptor-like 3 ELISA Kit; Nogo-66 receptor homolog 1 ELISA Kit; Nogo-66 receptor-related protein 2 ELISA Kit; NgR2 ELISA Kit

Abbreviation

RTN4RL2

Uniprot No.

Q86UN3

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RTN4RL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-106
1:2	Average %	95
	Range %	85-104
1:4	Average %	95
	Range %	86-100
1:8	Average %	96
	Range %	88-109

Recovery

The recovery of human RTN4RL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	84-107
EDTA plasma (n=4)	92	90-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.081	2.017	2.049	1.947
5	1.777	1.758	1.768	1.666
2.5	1.337	1.326	1.332	1.230
1.25	0.826	0.849	0.838	0.736
0.625	0.517	0.531	0.524	0.422
0.312	0.338	0.345	0.342	0.240
0.156	0.273	0.289	0.281	0.179
0	0.103	0.101	0.102

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human RTN4RL2 ELISA Kit was designed for the quantitative measurement of Human RTN4RL2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Background

Function
(From Uniprot)

Cell surface receptor that plays a functionally redundant role in the inhibition of neurite outgrowth mediated by MAG. Plays a functionally redundant role in postnatal brain development. Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum. Does not seem to play a significant role in regulating axon regeneration in the adult central nervous system. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development. Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton.

Gene References into Functions

Authors highlight the structural and biochemical aspects of the interaction of Nogo receptors (R1 and R2) with myelin inhibitors such as MAG, Nogo A and OMgp.[Review] PMID: 24956133
These results indicate that a lack of NgR1/2 expression promotes the adhesion of DCs to myelin. PMID: 21906273
predominantly expressed in the neurons of the embryonic and adult nervous systems PMID: 14664809
The release of N-terminal fragment-NgRH1 was blocked by a hydroxamate-based zinc metalloproteinase inhibitor and tissue inhibitor of metalloproteinases-2 and -3 PMID: 15629437
Nogo-66 receptor homolog 1 signaling regulates activity-dependent synaptic strength and neuronal plasticity. PMID: 18337405

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Membrane raft. Cell projection, dendrite. Perikaryon. Cell projection, axon.

Protein Families

Nogo receptor family

Tissue Specificity

Highly expressed in brain and liver. Expressed at lower levels in kidney, mammary gland, placenta, skeletal muscle, spleen and thyroid.

Database Links

HGNC: 23053

OMIM: 610462

KEGG: hsa:349667

STRING: 9606.ENSP00000335397

UniGene: Hs.502618

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Code	CSB-EL020576HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.