Human Retinoic Acid ELISA Kit

Human Retinoic Acid ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Human Retinoic Acid ELISA Kit

Name: Human Retinoic Acid ELISA Kit
Brand: CUSABIO
SKU: CSB-E16712h

Citations (7) Protocol

Code	CSB-E16712h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (7)
Related Products
Customer Reviews and Q&A

Target Name

Retinoic Acid

Abbreviation

Retinoic Acid

Species

Homo sapiens (Human)

Sample Types

Detection Range

Sensitivity

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	93
	Range %	86-100
1:200	Average %	88
	Range %	81-95
1:400	Average %	96
	Range %	89-102
1:800	Average %	85
	Range %	81-90

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

100

0.146

0.138

0.142

0.325

0.337

0.331

0.649

0.618

0.634

12.5

1.123

1.059

1.091

6.25

1.685

1.601

1.643

2.096

2.103

2.100

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

GCKIII Kinases Control Hepatocellular Lipid Homeostasis via Shared Mode of Action E Cansby, M Caputo, E Andersson, R Saghaleyni,Journal of Lipid Research,2024
Exploring common modulations induced by three fish RNA viruses in turbot (Scophthalmus maximus): Cholesterol, vitamin D3 and retinol metabolism as shared targets P Pereiro, A Figueras, B Novoa,Aquaculture,2024
All-Trans Retinoic Acid Prevents the Progression of Gastric Precancerous Lesions by Regulating Disordered Retinoic Acid Metabolism H Wu,Nutrition and cancer,2023
CD34+CD10+CD19− Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization A Asai,Cells,2022
Cellular retinol binding protein-1 inhibits cancer stemness via upregulating WIF1 to suppress Wnt/β-catenin pathway in hepatocellular carcinoma Xiangye Li,BMC Cancer,2021
CHARACTERISATION OF PATHWAYS INVOLVED IN BACTERIAL OR VIRAL INDUCED EXACERBATIONS OF AIRWAY DISEASES Mang S, et al,/,2019
Soluble Mucosal Addressin Cell Adhesion Molecule 1 and Retinoic Acid are Potential Tools for Therapeutic Drug Monitoring in Patients with Inflammatory Bowel Disease Treated with Vedolizumab: A Proof of Concept Study Stephane Paul.et al,Journal of Crohn's and Colitis,2018

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■ Q&A

Per customers request, please provide the following information regarding Human Retinoic Acid ELISA Kit (CSB-E16712h)
a. What were the antibodies used in this kit raised against?
b. What is the standard? (Recombinant full-length protein? Partial protein
c. (range?)?, peptide (sequence range?), native protein?)
e. If recombinant protein, then what is the host?
f. Is the capture antibody a monoclonal or polyclonal?
g. What is the host of the capture antibody?
h. What is the immunogen of the capture antibody?
i. Is the detection antibody a monoclonal or polyclonal?
j. What is the host of the detection antibody?
k. What is the immunogen of the detection antibody?
l. Can this assay be used with another species besides human?

Thanks for your inquiry! CSB-E16712h This kit is based on competitive method. Retinoic Acid is small molecule.The plate is coated with specific antibody for Retinoic Acid. It is mouse monoclonal antibody and immunogen is modified by special modification. Sorry we can not disclose how it is modified. The antigen in the sample and HRP conjugated antigen compete to bind with the specific antibody. Standard is purified Retinoic Acid small molecule.
Since for different species the sample matrix is different, we have developed Retinoic Acid elisa kits for different species. We suggest you choose the corresponding kit so as to avoid matrix interference.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.