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- An assay plate --- The 96-well plate has been pre-coated with the anti-mouse retinoic acid antibody.
- Lyophilized standard (1 x 200 μl) --- Reconstitute the standard with sample diluent at dilution series and draw the standard curve.
- HRP-conjugate retinoic acid (100 x concentrate) (1 x 60 μl)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use.
- HRP-avidin Diluent (1 x 10 ml) --- Dilute the HRP-avidin.
- Sample Diluent (2 x 20 ml) --- Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (25 x concentrate) (1 x 20 ml) --- Wash away the unbound solution and non-specific substances.
- TMB Substrate --- TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution (1 x 10 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microtiter plate when incubation.
- An instruction manual
