Human Secreted phosphoprotein 2 (SPP2/SPP24) ELISA kit

Instructions
Code CSB-EL022604HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name secreted phosphoprotein 2, 24kDa
Alternative Names SPP2 ELISA Kit; SPP24 ELISA Kit; Secreted phosphoprotein 24 ELISA Kit; Spp-24 ELISA Kit; Secreted phosphoprotein 2 ELISA Kit
Abbreviation SPP2
Uniprot No. Q13103
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 98
Range % 96-102
1:10 Average % 107
Range % 103-114
1:20 Average % 94
Range % 90-98
1:40 Average % 88
Range % 86-95
Recovery
The recovery of human SPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 99-108
EDTA plasma (n=4) 94 90-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1000 2.188 2.233 2.211 2.028
500 1.732 1.824 1.778 1.595
250 1.211 1.261 1.236 1.053
125 0.698 0.757 0.728 0.545
62.5 0.523 0.522 0.523 0.340
31.2 0.345 0.358 0.352 0.169
15.6 0.252 0.260 0.256 0.073
0 0.176 0.189 0.183  
.
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Could coordinate an aspect of bone turnover.
Gene References into Functions
  1. Spp24 can inhibit the growth of prostate cancer and its bone metastasis induced by BMP2; spp24 may have great potential to be a therapeutic agent in clinical situations PMID: 27793899
  2. the two mutations of SPP2 have dominant negative effects and cellular accumulation of Spp-24 might be particularly toxic to photoreceptors and/or retinal pigment epithelium. SPP2 has a new role in retinal degeneration. PMID: 26459573
  3. Report describes a C-terminal fragment within SPP24 that is distinct from the cystatin domain and which independently binds to BMP-2 and TGF-beta. This fragment inhibited BMP-2 activity in an ectopic bone forming assay. PMID: 25418420
  4. Spp24, or proteolytic products of Spp24, bind cytokines of the TGF-beta superfamily and also activate intracellular signaling pathways [review] PMID: 25339413
  5. Spp24 truncation products have effects on osteoblastic differentiation mediated by kinase pathways that are independent of exogenous BMP/TGF-beta cytokines. PMID: 25501958
  6. Results describe the localization of the human secreted phosphoprotein 24 gene, its structure and the start of transcription in liver. PMID: 15062857
  7. spp24 contains at least 2 single nucleotide polymorphisms. Given its mechanism of action and sequence variability, SPP24 may be an interesting candidate for future studies of the genetic regulation of bone mass. PMID: 19375587

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Subcellular Location Secreted
Protein Families SPP2 family
Tissue Specificity Detected in liver and plasma.
Database Links

HGNC: 11256

OMIM: 602637

KEGG: hsa:6694

STRING: 9606.ENSP00000168148

UniGene: Hs.444488

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