Human Selenoprotein P(SEPP1) ELISA kit

Code CSB-EL021018HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P49908
Lead Time 7-14 working days
Abbreviation SEPP1
Target Name selenoprotein P, plasma, 1
Alias SELP, SeP, selenoprotein P
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3 UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 85  
Range % 80-92  
1:400 Average % 99  
Range % 93-105  
1:800 Average % 92  
Range % 85-99  
1:1600 Average % 95  
Range % 86-98  
Recovery
The recovery of human SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 87-101  
EDTA plasma (n=4) 100 94-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.483 2.476 2.480 2.396  
5 1.876 1.896 1.886 1.802  
2.5 1.270 1.304 1.287 1.203  
1.25 0.716 0.768 0.742 0.658  
0.625 0.512 0.536 0.524 0.440  
0.312 0.288 0.296 0.292 0.208  
0.15 0.211 0.229 0.220 0.136  
0 0.082 0.086 0.084    
References
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Function Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
Subcellular Location Secreted
Protein Families Selenoprotein P family
Tissue Specificity Made in the liver and heart and secreted into the plasma. It is also found in the kidney.
Database Links

HGNC: 10751

OMIM: 601484

KEGG: hsa:6414

STRING: 9606.ENSP00000420939

UniGene: Hs.275775

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