Human Selenoprotein P(SEPP1) ELISA kit

Instructions
Code CSB-EL021018HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name selenoprotein P, plasma, 1
Alternative Names SELP, SeP, selenoprotein P
Abbreviation SEPP1
Uniprot No. P49908
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 85  
Range % 80-92  
1:400 Average % 99  
Range % 93-105  
1:800 Average % 92  
Range % 85-99  
1:1600 Average % 95  
Range % 86-98  
Recovery
The recovery of human SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 87-101  
EDTA plasma (n=4) 100 94-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.483 2.476 2.480 2.396  
5 1.876 1.896 1.886 1.802  
2.5 1.270 1.304 1.287 1.203  
1.25 0.716 0.768 0.742 0.658  
0.625 0.512 0.536 0.524 0.440  
0.312 0.288 0.296 0.292 0.208  
0.15 0.211 0.229 0.220 0.136  
0 0.082 0.086 0.084    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Target Data

Function Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
Subcellular Location Secreted
Protein Families Selenoprotein P family
Tissue Specificity Made in the liver and heart and secreted into the plasma. It is also found in the kidney.
Database Links

HGNC: 10751

OMIM: 601484

KEGG: hsa:6414

STRING: 9606.ENSP00000420939

UniGene: Hs.275775

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