Human Serum amyloid A-4 protein(SAA4) ELISA kit

Code CSB-EL020659HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P35542
Lead Time 5-7 working days
Abbreviation SAA4
Protein Biological Process 1 Immunity
Target Name serum amyloid A4, constitutive
Alias C-SAA, CSAA,
Species Homo sapiens (Human)
Protein Biological Process 3 Acute phase
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 10516
RGD 1307371
MGI 98224
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SAA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 97  
Range % 93-101  
1:400 Average % 100  
Range % 95-104  
1:800 Average % 95  
Range % 89-99  
1:1600 Average % 99  
Range % 94-104  
Recovery
The recovery of human SAA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-97  
EDTA plasma (n=4) 92 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.845 2.765 2.805 2.702  
200 2.147 2.089 2.118 2.015  
100 1.422 1.437 1.430 1.327  
50 0.730 0.712 0.721 0.618  
25 0.394 0.376 0.385 0.282  
12.5 0.237 0.242 0.240 0.137  
6.25 0.153 0.147 0.150 0.047  
0 0.101 0.105 0.103    
References
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Function Major acute phase reactant. Apolipoprotein of the HDL complex.
Subcellular Location Secreted
Protein Families SAA family
Tissue Specificity Expressed by the liver; secreted in plasma.
Database Links

HGNC: 10516

OMIM: 104752

KEGG: hsa:6291

STRING: 9606.ENSP00000278222

UniGene: Hs.1955

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