Human Serum amyloid A-4 protein(SAA4) ELISA kit

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Human Serum amyloid A-4 protein(SAA4) ELISA kit

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Code	CSB-EL020659HU
Size	96T,5×96T,10×96T
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Alternative Names

Amyloid A; serum; 4 ELISA Kit; C SAA ELISA Kit; C-SAA ELISA Kit; Constitutively expressed serum amyloid A protein ELISA Kit; CSAA ELISA Kit; SAA 4 ELISA Kit; Saa4 ELISA Kit; SAA4 protein ELISA Kit; SAA4_HUMAN ELISA Kit; Serum amyloid A 4 protein ELISA Kit; Serum amyloid A-4 protein ELISA Kit; Serum amyloid A4 constitutive ELISA Kit

Abbreviation

SAA4

Uniprot No.

P35542

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 ng/ml-1000 ng/ml.

Sensitivity

3.9 ng/ml.

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SAA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	97
	Range %	93-101
1:200	Average %	100
	Range %	95-104
1:400	Average %	95
	Range %	89-99
1:800	Average %	99
	Range %	94-104

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

1000

2.845

2.765

2.805

2.702

500

2.147

2.089

2.118

2.015

250

1.422

1.437

1.430

1.327

125

0.730

0.712

0.721

0.618

62.5

0.394

0.376

0.385

0.282

31.2

0.237

0.242

0.240

0.137

15.6

0.153

0.147

0.150

0.047

0.101

0.105

0.103

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia Kong Z, et al,Animals,2019

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Major acute phase reactant.

Gene References into Functions

Data identified SAA4 as a protein that was positively correlated with RF and RA. SAA4 may represent a novel prescreening marker for the diagnosis of RA. PMID: 28505104
expressed in invasive trophoblast cells PMID: 24951172
Six apolipoproteins (APOA1, APOA2, APOB, APOC2, APOC3, and APOE) were able to differentiate bladder cancer from hernia. SAA4 was significantly increased in bladder cancer subgroups, whereas ProEGF was significantly decreased in bladder cancer subgroups. PMID: 23631828
studies provide the first evidence for a novel type of AA amyloidosis in which the fibrils were formed from a mutated SAA4 protein PMID: 20536400
The sequences of 1478 and 1836 bp of the SAA1 and SAA4 5'-flanking regions were determined. PMID: 12410800

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Subcellular Location

Secreted.

Protein Families

SAA family

Tissue Specificity

Expressed by the liver; secreted in plasma.

Database Links

HGNC: 10516

OMIM: 104752

KEGG: hsa:6291

STRING: 9606.ENSP00000278222

UniGene: Hs.1955

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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