Human Sialidase ELISA Kit

Code CSB-E13811h
Size 96T,5×96T,10×96T
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Product Details

Target Name
sialidase 1 (lysosomal sialidase)
Alternative Names
Acetylneuraminyl hydrolase ELISA Kit; exo-alpha-sialidase ELISA Kit; G9 sialidase ELISA Kit; Lysosomal sialidase ELISA Kit; N acetyl alpha neuraminidase 1 ELISA Kit; N-acetyl-alpha-neuraminidase 1 ELISA Kit; NANH ELISA Kit; NEU ELISA Kit; NEU1 ELISA Kit; NEUR1_HUMAN ELISA Kit; Neuraminidase 1 ELISA Kit; Neuraminidase ELISA Kit; SIAL1 ELISA Kit; sialidase 1 (lysosomal sialidase) ELISA Kit; Sialidase 1 ELISA Kit; Sialidase; lysosomal ELISA Kit; Sialidase-1 ELISA Kit
Abbreviation
NEU1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, urine, cerebrospinal fluid (CSF)
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sialidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-90  
1:2 Average % 94  
Range % 91-98  
1:4 Average % 98  
Range % 92-105  
1:8 Average % 95  
Range % 86-100  
Recovery
The recovery of human sialidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 80-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.621 2.522 2.572 2.460  
10 2.212 2.112 2.162 2.050  
5 1.701 1.654 1.678 1.566  
2.5 1.257 1.197 1.227 1.115  
1.25 0.763 0.741 0.752 0.640  
0.625 0.461 0.434 0.448 0.336  
0.312 0.287 0.273 0.280 0.168  
0 0.115 0.109 0.112    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human NEU1 ELISA Kit was designed for the quantitative measurement of Human NEU1 protein in serum, urine, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

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Target Background

Function
(From Uniprot)
Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
Gene References into Functions
  1. in monocytes/macrophages, NEU1 is interconnected in a positive feedback loop with lipopolysaccharides and IL-1beta as enhancers of inflammation, and may therefore promote atherosclerosis and plaque instability PMID: 29371126
  2. Patients with minimally invasive and lepidic adenocarcinomas were categorized as low-risk group with high EGFR mutation rate; Patients with micro-papillary and solid adenocarcinomas were categorized as high-risk with lower EGFR mutation rate PMID: 29496311
  3. Transmembrane topology and dimerization ability of NEU1 have been reported. PMID: 27917893
  4. this work identified NEU1 as a novel biomarker for both diagnosis and prognosis in hepatocellular carcinoma PMID: 27602751
  5. Our combined data indicate that as HPMECs achieve confluence and CD31 ectodomains become homophilically engaged, multiple SFKs are activated to increase tyrosine phosphorylation of p120ctn, which in turn, functions as a cross-bridging adaptor molecule that physically couples NEU1 to CD31, permitting NEU1-mediated desialylation of CD31. PMID: 28343945
  6. TLR4 protein PMID: 29375116
  7. neuraminidase-1 (Neu1) in complex with matrix metalloproteinase-9 and G protein-coupled receptor tethered to RTKs and TLRs is identified as a major target in multistage tumorigenesis [review] PMID: 27029067
  8. EGFR protein, human is predictive of adjuvant chemo response. Also, It is a prognostic factor. PMID: 28862415
  9. Data suggest that mutations c.90delC (p.Tyr30Ter) and c.572A>T (p.Asp191Val) of the NFU1 gene probably underlie the pathogenesis of multiple mitochondrial dysfunction syndrome (MMDS). PMID: 28186588
  10. Elevated NEU1 expression alters functional activities of distinct lung cell types in vitro and recapitulates lymphocytic infiltration and collagen accumulation in vivo, consistent with mechanisms implicated in lung fibrosis. PMID: 26993524
  11. initial treatment with an EGFR-TKI is a reasonable option for patients presenting with EGFR mutation-positive NSCLC and a CM. PMID: 28237165
  12. miR-125b functions as an oncogene in gastric cancer and represents a new potential therapeutic target for gastric cancer. PMID: 27220320
  13. NEU1 siRNA can effectively inhibit proliferation, apoptosis, and invasion of human ovarian cancer cells by targeting lysosome and oxidative phosphorylation signaling, which can serve as a new target ovarian cancer treatment PMID: 26463994
  14. How the point mutations of the neuraminidase sequences affected the susceptibility of H9N2 virus to oseltamivir is still to be determined and deserve further investigations PMID: 26455375
  15. Our study shows that the 340-cavity is not an occasional or atypical domain in NA subtypes, and it has potential to function as a new hotspot for influenza drug binding. PMID: 26768362
  16. Basal Neu-1 catalytic activity is strongly increased in aged cells. PMID: 26086247
  17. Neu1 desialylation is a mechanism of Fc-independent platelet clearance in immune thrombocytopenia. PMID: 26185093
  18. Muc1 clearly plays a significant role in enhancing the hypoxia-inducible transcription factors protective pathway during ischemic insult and recovery in kidney epithelia PMID: 25925251
  19. This study analyzed patient cells with GM1 gangliosidosis and sialidosis. A novel mutation p.R347Q is identified in NEU1 gene. PMID: 25600812
  20. The lymphocyte levels of NEU1 and ST6GAL1 mRNA expression are significantly increased in erythremia. PMID: 25566667
  21. Insulin-induced phosphorylation of the insulin receptor is dependent on Neu1 sialidase activity. PMID: 24583283
  22. Sialidosis should be suspected and the NEU1 gene analyzed in patients with isolated action myoclonus presenting in adulthood in the absence of other typical clinical and laboratory findings. PMID: 24808020
  23. catalytically active NEU1 inhibits in vitro angiogenesis through desialylation of its substrate, CD31 PMID: 24550400
  24. Three novel mutations in the NEU1 gene in two Indian patients of sialidosis, one with the type I form and the other with the infantile type II form are reported here. PMID: 23391804
  25. NEU1 gene missense mutation was found in a patient diagnosed with type I sialoidosis. PMID: 23291686
  26. Weak anchorage of NEU1 and NEU3 to the plasma membrane and their loss during erythrocyte life could be a tool to preserve the cellular sialic acid content to avoid early aging of erythrocyte and processes of cell aggregation in the capillaries. PMID: 22903576
  27. Data indicate that sialidases Neu1 and Neu3 are present on sperm, and their activity is required for capacitation and zona pellucida binding. PMID: 22989879
  28. Data show that NEU1 was immunolocalized to both the plasma membrane and the perinuclear region, and NEU3 was detected both in the cytosol and nucleus. PMID: 22403397
  29. Neuraminidase 1 as a modulator of cell receptors. (Review) PMID: 21928149
  30. a novel Neu1 and MMP9 cross-talk in alliance with TLR4 on the cell surface that is essential for ligand activation of TLRs and subsequent cellular signaling. PMID: 21873432
  31. colocalized with CD18 in PMNs; could be a physiologic source of the enzymatic activity that removes sialyl residues on beta2 integrin and ICAM-1, resulting in their enhanced interaction PMID: 21551251
  32. Data show that Neu-1 siRNA blocks the process of GM(3)/LacCer conversion. PMID: 21103358
  33. Summary of NEU1 mutations found in sialidosis patients. PMID: 14517945
  34. type II sialidosis associated mutations in NEU1 affect lysosomal sialidase activity. PMID: 14695530
  35. Data show that the differentiation of monocytes into macrophages is associated with regulation of the expression of at least three distinct cellular sialidases, Neu1, 3, and 4, with specific up-regulation of the enzyme activity of only Neu1. PMID: 15885103
  36. Lysosomal sialidase (neuraminidase-1) is targeted to the cell surface to facilitate elastic fiber assembly PMID: 16314420
  37. effects of GLB1, PPCA and NEU1 gene mutations on elastogenesis in skin fibroblasts PMID: 16538002
  38. upregulation of the Neu1 expression is important for the primary function of macrophages and there is a link between Neu1 and the cellular immune response PMID: 16835219
  39. Activation of lymphocytes resulted in a ninefold increase in Neu1-specific activity after growth of cells in culture for 5 days.Cell surface Neu1 was found tightly associated with a subunit of protective protein/cathepsin A (PPCA). PMID: 17028199
  40. Female cervical mucus contains an endogenous menstrual cycle-related sialidase that could be involved in modifying the rheologic properties of mucus to favor sperm progression at fertilization. PMID: 17562335
  41. NEU1 is a negative regulator of lysosomal exocytosis. PMID: 18606142
  42. related to malignancy and may be potential targets for cancer diagnosis and therapy PMID: 18651674
  43. Neuraminidase caused the desialylation of both PDGF and IGF-1 receptors and diminished the intracellular signals induced by the mitogenic ligands PDGF-BB and IGF-2. PMID: 18772331
  44. results suggest that NEU1 is important in regulation of integrin beta4-mediated signaling, leading to suppression of metastasis PMID: 19151752
  45. findings show homozygous mutations of NEU1 544A-->G (Ser182Gly substitution) play a major role of the genotype and phenotype of Sialidosis type 1 PMID: 19473359
  46. The genetic characterization of the two patients showed one known [c. 679G > A (p.G227R)] NEU1 missense mutation (detected in P2), and the new c.807 + 1G > A splicing defect PMID: 19568825
  47. Results describe the hydrodynamic properties of PPCA, NEU1, and a complex of the two proteins and identified multiple binding sites on both proteins. PMID: 19666471
  48. occurrence of Neu3 in the inner membrane and Neu1 in the outer membrane of the nuclear envelope PMID: 19686243

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Involvement in disease
Sialidosis (SIALIDOSIS)
Subcellular Location
Lysosome membrane; Peripheral membrane protein; Lumenal side. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Lysosome. Note=Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.
Protein Families
Glycosyl hydrolase 33 family
Tissue Specificity
Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain.
Database Links

HGNC: 7758

OMIM: 256550

KEGG: hsa:4758

STRING: 9606.ENSP00000364782

UniGene: Hs.520037

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