Human Small proline-rich protein 2B(SPRR2B) ELISA kit

Code CSB-EL022613HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name small proline-rich protein 2B
Alternative Names SPRR2B ELISA Kit; Small proline-rich protein 2B ELISA Kit; SPR-2B ELISA Kit
Abbreviation SPRR2B
Uniprot No. P35325
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human SPRR2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %90
Range %87-96
1:2Average %103
Range %99-105
1:4Average %93
Range %87-97
1:8Average %91
Range %88-96
The recovery of human SPRR2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-101
EDTA plasma (n=4)9086-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
20002.221 2.161 2.191 2.085
10001.864 1.781 1.823 1.717
5001.425 1.546 1.486 1.380
2501.212 1.157 1.185 1.079
1250.746 0.795 0.771 0.665
62.50.357 0.350 0.354 0.248
31.250.236 0.250 0.243 0.137
00.104 0.108 0.106
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

(From Uniprot)
Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.
Gene References into Functions
  1. Knockdown of the Sprr2b gene or inhibition of SPRR2B phosphorylation attenuates USP7/MDM2 binding and p53 degradation, leading to fibroblasts cell cycle arrest. PMID: 29581288
  2. An SNP in SPRR2B was predictive of asthma among white children with eczema from 2 independent populations. PMID: 22374195
  3. We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
  4. SPR2B transcripts and protein were moderately increased in the human papillomaviurs 11- and 59-infected tissues. PMID: 14748073
Subcellular Location Cytoplasm
Protein Families Cornifin (SPRR) family
Tissue Specificity Suprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
Database Links

HGNC: 11262

OMIM: 182268

KEGG: hsa:6701

STRING: 9606.ENSP00000340703

UniGene: Hs.568239


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