Name: Human Soluble protein-100B,S-100B ELISA Kit
Brand: CUSABIO
SKU: CSB-E08065h

Product Details

Target Name

S100 calcium binding protein B

Alternative Names

NEF ELISA Kit; Protein S100 B ELISA Kit; Protein S100-B ELISA Kit; S 100 calcium binding protein beta chain ELISA Kit; S 100 protein beta chain ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 ELISA Kit; S100 calcium binding protein beta (neural) ELISA Kit; S100 calcium-binding protein B ELISA Kit; S100 protein beta chain ELISA Kit; S100B ELISA Kit; S100B_HUMAN ELISA Kit; S100beta ELISA Kit

Abbreviation

S100B

Uniprot No.

P04271

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-95
1:2	Average %	89
	Range %	85-94
1:4	Average %	95
	Range %	92-100
1:8	Average %	90
	Range %	85-98

Recovery

The recovery of human S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-99
EDTA plasma (n=4)	96	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.037	1.902	1.970	1.809
2500	1.585	1.583	1.584	1.423
1250	1.184	1.156	1.170	1.009
625	0.821	0.852	0.837	0.676
312	0.595	0.581	0.588	0.427
156	0.372	0.380	0.376	0.215
78	0.249	0.234	0.242	0.081
0	0.164	0.158	0.161

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human S100B antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled S100B antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

S100 calcium binding protein B (S100B) is a small acidic protein belonging to the S100 family of calcium-binding proteins. Glial cells and Schwann cells of the nervous system primarily express S100B, where it plays important roles in intracellular calcium homeostasis, cell cycle regulation, and cytoskeletal dynamics. Under pathological conditions, S100B is released into extracellular spaces and can serve as a biomarker for neuronal damage. This makes it valuable in research examining brain injury, neurodegenerative diseases, and neuroinflammation.

The Human Soluble protein-100B,S-100B ELISA Kit (CSB-E08065h) is designed for quantitative measurement of S100B in human samples. This sandwich ELISA accommodates serum, plasma, tissue homogenates, and cerebrospinal fluid with a detection range of 78 pg/mL to 5000 pg/mL and sensitivity of 19.5 pg/mL. The assay requires 50-100 μL sample volume, measures absorbance at 450 nm, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in clinical research studies to measure S100 calcium binding protein B levels in human biological samples. The applications span neurological research contexts, including studies of brain injury biomarkers and complement system activation following cardiac arrest resuscitation.

• Neurological biomarker research: Measuring brain-specific protein levels in serum samples from patients undergoing surgical procedures, as part of comprehensive biochemical parameter panels
• Cardiac arrest studies: Measuring neurological damage markers in serum samples collected at multiple time points following return of spontaneous circulation, alongside complement system and inflammatory biomarkers
• Brain injury research: Analysis of plasma levels in conjunction with other neurological biomarkers including amyloid beta proteins and tau protein for comprehensive neurological profiling
• Multi-biomarker panels: Integration into broader biochemical screening approaches that simultaneously evaluate multiple neurological and inflammatory markers in clinical research settings

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Customer Reviews and Q&A

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■ Q&A

1.Hi I am wondering if there is a recommended starting dilution for plasma for S100B ELISA CSB-E08065h ?
2.Is the assay compatible with sodium citrate as anticoagulant for plasma ?

Thanks for your inquiry. The concentration of S-100β in cerebrospinal fluid was a useful marker for investigating
the degree of brain damage after head injury, cerebral hemorrhage and ischemic stroke. Recently another
report described that the increasing of S-100β in blood correlated to the degree of brain damage after
cerebral ischemia, infarction, hemorrhage and severe head injury. For CSB-E08065h Human Soluble protein-100B,S-100B ELISA Kit, dilution is not needed if you test normal serum or plasma samples.If you use sodium citrate as anticoagulant for plasma, there will be no much influence. We recommend you use EDTA plasma and we validated this before.

Target Background

Function
(From Uniprot)

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

Gene References into Functions

rs9722, a functional SNP in the 3'-UTR of the S100B gene, was strongly associated with age of onset of Parkinson's disease. PMID: 29529989
The S100B gene rs9722 polymorphism may contribute to the susceptibility of IS. PMID: 29343763
It is unclear if the higher S100B concentration in football players playing at least 50 plays was caused by exercise or impacts. Therefore, if serum S100B is to be used as a biomarker of impacts, and possible brain injury in sport, exercise time and intensity should be taken into account as confounding variables. PMID: 30203865
High S100B expression is associated with chemoresistance in ovarian cancer. PMID: 29956807
The serum levels of S100B in Groups A and B rose after the injury and reached a peak on the 4th day of injury. Serum levels of NSE and S100B protein can reflect the degree of spinal cord injury. PMID: 29959890
Serum levels of S100B (and neuron-specific enolase) at admission were related to initial Glasgow Coma Scale (GSC) scores, and these levels one week after traumatic brain injury were related to six month GSC scores. PMID: 30257306
Studied use of serum S100B as a biomarker to predict injury in patients with mild head injury. Found patients with cranial injury detected by CT, had higher S100B protein levels than those without cranial injury. PMID: 29850551
The expression of serum S100beta in patients with postoperative cognitive dysfunction was significantly increased PMID: 28537663
A high S100B expression is associated with a good prognosis in patients with p53 mutant and p53 wild-type breast cancers. PMID: 29345293
S100B may have a role in pathogenesis of schizophrenia; patients show higher plasma levels of S100B than healthy controls PMID: 27279465
We investigated the possible correlation between TCD results and S100B, NSE and LDH levels as well as between NSE-LDH and S100B-LDH levels by regression analysis. We found a statistically significant relationship for both NSE, S100B with LDH. PMID: 29244749
Results showed that our prepared S100B mAbs were suitable for detecting S100B expression in human tissues, furnishing promising tools for further functional investigation and clinical applications. PMID: 28849099
In this study demonstrated that S100b is elevated in Alzheimer disease cases. and the increased levels in African Americans here may be indicative of increased severity in specific populations. PMID: 28582866
In neonates, NSE and s100B levels increase after bypass surgery and return below preoperative baseline levels by postoperative day seven. The levels of s100B were positively correlated with circulatory arrest time and negatively correlated with age at time of surgery. PMID: 28571730
the serum levels of S100B protein mediated the association between S100B gene polymorphism and scene selectivity in the retrosplenial cortex PMID: 26733530
There is a significant correlation between mortality in the critically ill patients in the intensive care unit and increased serum concentration of S100B and NSE. PMID: 29222857
Our findings showed that both S100beta and NSE levels similarly increased during CPB and immediately after CPB during sevoflurane and propofol based anesthesia. PMID: 26856295
This study demonstrated that S100A12 mRNA levels were significantly decreased in the new cases of untreated MS patients in comparison to healthy controls. PMID: 28433998
Increased serum levels of S100B protein (and NSE) were observed postoperatively in patients with postoperative cognitive dysfunction. PMID: 27433840
high S100B expression in Multiple Sclerosis (MS) patient samples suggests its usefulness as a diagnostic biomarker for MS. PMID: 26184632
the underlying mechanism of S100B-mediated effects on cancer stem-like cell stemness was not dependent on its binding with a receptor for advanced glycation end products (RAGE), but might be through intracellular regulation, through the inhibition of p53 expression and phosphorylation. PMID: 27501952
Data show that a high serum S100beta protein level, hypertension, and high low density lipoprotein-cholesterol (LDL-C) level were the independent risk factors for cerebral small vessel disease (SVD). PMID: 28143956
the serum concentrations of S100B are increased in bipolar disorder patients. PMID: 27475892
Levels of NSE and S100B are still elevated 1 year postpartum in women who have had preeclampsia in contrast to women with previous normal pregnancies. PMID: 27653032
In vitro experiments using repeated freeze-thaw procedures showed an intracellular up-regulation of S100B in normal and vitiligo melanocytes before an extensive release in the environment. PMID: 28212812
Acute paranoid schizophrenia inpatients present a day/night change of S100B serum levels at admission that disappears at discharge. PMID: 28188811
Results show that S100beta protein levels are increased in childhood-onset SLE with cognitive impairment suggesting that elevated S100beta protein levels were associated with cognitive impairment. PMID: 28394234
Compared to the control, the S100B/GAPDH mRNA ratio was significantly decreased in chronic liver diseases (p = 0.006) and most decreased in chronic hepatitis C patients (p = 0.023). We here evaluated S100B expression as a marker of hepatic nerves in patients with various chronic liver diseases, topographically and semi-quantitatively. PMID: 27255110
results indicate there were decreased S100B serum levels in bipolar patients in a manic phase after treatment. PMID: 28088747
Studies indicate that S100 calcium binding protein B protein (S100B) has high affinity for zinc (Zn2+). PMID: 27430886
S100beta has potential as a new surveillance tool for patients with ER-positive breast cancer to monitor endocrine therapy. PMID: 28399921
For patients recruited PMID: 28419114
Our study provides information on the dynamic changes of MMP-9-TIMP-1 system and S100B in the perioperative period. Preoperative reduction of TIMP-1 might be predictive for shunt requirement but future studies are required for verification. PMID: 27121520
this study shows that low levels of S100B correlate to a better anti-CTLA-4 immunotherapy response and survival in advanced melanoma patients PMID: 27728898
Our data suggest that elevated S100B protein levels might indicate neuronal damage in the brains of people with cannabis use disorder. PMID: 26624020
The strong CD68 and S100 co-expression in our case did not allow a clear-cut discrimination between the immunophenotype of histiocytic neoplasms and amelanotic melanoma, because of CD68 immunoreactivity occurring in 75% of metastatic malignant melanomas PMID: 26407839
Cognitive deterioration could not be predicted using perioperative embolic load and S-100beta changes after carotid revascularization. PMID: 27395808
Serum S100B protein levels increased after general anaesthesia. The values of S100Bp (median 164.0 ng/L) were significantly higher than the values of S100Bb (median 94.5 ng/L). The median of the difference between S100Bp and S100Bb was 58.0 ng/L. There were statistically significant differences between S100Bb and S100Bp. PMID: 26969427
Although an elevation in S100B was not observed in this (second) ascent during re-exposure to the hypobaric hypoxia of high altitude for either the non-treated (acclimatised) or the dexamethasone treated (acclimatised and dexamethasone) groups, the hypothesis that S100B would reduce following acclimatisation could not be statistically confirmed in our study given the wide confidence intervals and small sample size PMID: 26924650
Serum S100B is a useful quantitative biomarker in routine follow-up of high-risk melanoma patients. PMID: 26819111
serum levels may be correlated with the carbon monoxide poisoning and could be biomarker for early diagnosis and treatment monitoring PMID: 25925764
Our results support the hypothesis that S-100beta is an important biological indicator of HIE and serum S-100beta levels can be used as a reference index to assess hypoxic-ischemic encephalopathy severity. PMID: 26600537
There was no association found between serum and CSF S100B with radiographic vasospasm or DIND. S100B is a potential prognostic marker for aSAH outcome PMID: 27007976
Fetuses of active smoker mothers had lower cord blood S100B levels compared to fetuses of non-smoker mothers. PMID: 24668715
This study inquires into the physiological concentrations of NSE and S100B proteins in CSF, carried out on a sufficiently large group of 601 patients. PMID: 26421286
Studied circulating S100B and adiponectin in children who underwent open heart surgery. S100B showed a significant increase from hospital admission up to 24 h after procedure. PMID: 26417594
Concentrations of serum S100B protein in brain-dead organ donors are extremely high and may support the diagnosis of brain death. PMID: 26401738
long-term exposure to high blood glucose concentrations leads to an increase in total oxidant in patients with diabetic ketoacidosis ( and that the neurotransmitter changes that develop in response to this exposure lead to an increase in S100B levels PMID: 26316432
plasma biomarkers S100-B and copeptin did not improve prediction of poor outcome following seizure PMID: 25849778
S100B serum levels are elevated in cerebral small vessel disease patients. PMID: 25990800

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

S-100 family

Tissue Specificity

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Database Links

HGNC: 10500

OMIM: 176990

KEGG: hsa:6285

STRING: 9606.ENSP00000291700

UniGene: Hs.422181

Code	CSB-E08065h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.