Human Tenascin-N(TNN) ELISA kit

Code CSB-EL024008HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9UQP3
Lead Time 7-14 working days
Abbreviation TNN
Target Name tenascin N
Alias TN-W,
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 22942
RGD 1306002
MGI 2665790
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %88-99
1:2Average %102
Range %97-105
1:4Average %94
Range %88-98
1:8Average %98
Range %93-102
Recovery
The recovery of human TNN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9286-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.022 2.001 2.012 1.855
51.733 1.812 1.773 1.616
2.51.307 1.288 1.298 1.141
1.250.989 0.973 0.981 0.824
0.6250.673 0.666 0.670 0.513
0.3120.466 0.454 0.460 0.303
0.1560.301 0.312 0.307 0.150
00.156 0.158 0.157
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Function Extracellular matrix protein that seems to be a ligand for ITGA8
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Tenascin family
Tissue Specificity Not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested (PubMed:17909022, PubMed:19884327). In brain tumors, detected around the endothe
Database Links

HGNC: 22942

OMIM: 617472

KEGG: hsa:63923

STRING: 9606.ENSP00000239462

UniGene: Hs.156369

Pathway PI3K-Akt signaling pathway
Focal adhesion

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