Human Tenascin-N(TNN) ELISA kit

Instructions
Code CSB-EL024008HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tenascin N
Alternative Names TNN ELISA Kit; TNW ELISA Kit; Tenascin-N ELISA Kit; TN-N ELISA Kit; Tenascin-W ELISA Kit; TN-W ELISA Kit
Abbreviation TNN
Uniprot No. Q9UQP3
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %88-99
1:2Average %102
Range %97-105
1:4Average %94
Range %88-98
1:8Average %98
Range %93-102
Recovery
The recovery of human TNN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9286-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.022 2.001 2.012 1.855
51.733 1.812 1.773 1.616
2.51.307 1.288 1.298 1.141
1.250.989 0.973 0.981 0.824
0.6250.673 0.666 0.670 0.513
0.3120.466 0.454 0.460 0.303
0.1560.301 0.312 0.307 0.150
00.156 0.158 0.157
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Extracellular matrix protein that seems to be a ligand for ITGA8
Gene References into Functions
  1. This study demonstrated that the genetic defect was totally or partly clarified in 21 patients with nine of them having potential disease-causing mutations in TTN. PMID: 26627873
  2. Results show that tenascin-W acts as a niche component for breast cancer metastasis to bone by supporting cell migration and cell proliferation of the cancer cells. PMID: 25868708
  3. The expression of tenascin-W is dependent on p38MAPK and JNK signaling pathways in mammary tumors. (tenascin-W) PMID: 15592496
  4. data imply that tenascin-W expression in the activated tumor stroma facilitates tumorigenesis by supporting the migratory behavior of breast cancer cells PMID: 17909022
  5. results reveal a clear association between elevated levels of tenascin-W and the presence of colorectal cancer and breast cancer PMID: 18306355

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Tenascin family
Tissue Specificity Not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested (PubMed:17909022, PubMed:19884327). In brain tumors, detected around the endothe
Database Links

HGNC: 22942

OMIM: 617472

KEGG: hsa:63923

STRING: 9606.ENSP00000239462

UniGene: Hs.156369

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