Human Tenascin-N(TNN) ELISA kit

Code CSB-EL024008HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
tenascin N
Alternative Names
TNN ELISA Kit; TNW ELISA Kit; Tenascin-N ELISA Kit; TN-N ELISA Kit; Tenascin-W ELISA Kit; TN-W ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156 ng/mL-10 ng/mL
0.039 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human TNN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %95
Range %88-99
1:2Average %102
Range %97-105
1:4Average %94
Range %88-98
1:8Average %98
Range %93-102
The recovery of human TNN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9286-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
102.022 2.001 2.012 1.855
51.733 1.812 1.773 1.616
2.51.307 1.288 1.298 1.141
1.250.989 0.973 0.981 0.824
0.6250.673 0.666 0.670 0.513
0.3120.466 0.454 0.460 0.303
0.1560.301 0.312 0.307 0.150
00.156 0.158 0.157
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human TNN ELISA Kit was designed for the quantitative measurement of Human TNN protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

(From Uniprot)
Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1. Involved in neurite outgrowth and cell migration in hippocampal explants. During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells. Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells.
Gene References into Functions
  1. This study demonstrated that the genetic defect was totally or partly clarified in 21 patients with nine of them having potential disease-causing mutations in TTN. PMID: 26627873
  2. Results show that tenascin-W acts as a niche component for breast cancer metastasis to bone by supporting cell migration and cell proliferation of the cancer cells. PMID: 25868708
  3. The expression of tenascin-W is dependent on p38MAPK and JNK signaling pathways in mammary tumors. (tenascin-W) PMID: 15592496
  4. data imply that tenascin-W expression in the activated tumor stroma facilitates tumorigenesis by supporting the migratory behavior of breast cancer cells PMID: 17909022
  5. results reveal a clear association between elevated levels of tenascin-W and the presence of colorectal cancer and breast cancer PMID: 18306355

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Tenascin family
Tissue Specificity
Not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested. In brain tumors, detected around the endothelial cell layer of the clood vessel
Database Links

HGNC: 22942

OMIM: 617472

KEGG: hsa:63923

STRING: 9606.ENSP00000239462

UniGene: Hs.156369

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