Human Transmembrane protein 119(TMEM119) ELISA kit

Code CSB-EL023686HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
transmembrane protein 119
Alternative Names
TMEM119 ELISA Kit; PSEC0199 ELISA Kit; UNQ731/PRO1415 ELISA Kit; Transmembrane protein 119 ELISA Kit; Osteoblast induction factor ELISA Kit; OBIF ELISA Kit
Abbreviation
TMEM119
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM119 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 107
Range % 103-110
1:2 Average % 88
Range % 84-92
1:4 Average % 107
Range % 103-110
1:8 Average % 90
Range % 87-93
Recovery
The recovery of human TMEM119 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 100-108
EDTA plasma (n=4) 88 84-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 1.805 1.831 1.818 1.691
2000 1.501 1.600 1.551 1.424
1000 1.282 1.198 1.240 1.113
500 1.003 1.068 1.036 0.909
250 0.704 0.712 0.708 0.581
125 0.471 0.498 0.485 0.358
62.5 0.356 0.362 0.359 0.232
0 0.126 0.128 0.127  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TMEM119 ELISA Kit was designed for the quantitative measurement of Human TMEM119 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts. May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation.
Gene References into Functions
  1. TMEM119 expression in osteosarcoma tissues is positively correlated with cell cycle, apoptosis, metastasis and TGF-beta signaling. PMID: 28496199
  2. TMEM119 serves as a reliable microglial marker that discriminates resident microglia from blood-derived macrophages in the human brain PMID: 26250788
  3. qPCR showed that TMEM119 expression was highly enriched in temporal lobe CD11b+ cells over unpurified whole brain, and barely detectable in peripheral blood leukocytes. PMID: 26884166
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cytoplasm. Endoplasmic reticulum membrane.
Tissue Specificity
Expressed in brain microglia (at protein level). Elevated expression levels seen in the brain of patients with Alzheimer disease. Expressed by osteoblast-like cells in bone tissues and follicular dendritic cells in lymphoid tissues.
Database Links

HGNC: 27884

KEGG: hsa:338773

UniGene: Hs.449718

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1