Human Transmembrane protein 119(TMEM119) ELISA kit

Instructions
Code CSB-EL023686HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name transmembrane protein 119
Alternative Names TMEM119 ELISA Kit; PSEC0199 ELISA Kit; UNQ731/PRO1415 ELISA Kit; Transmembrane protein 119 ELISA Kit; Osteoblast induction factor ELISA Kit; OBIF ELISA Kit
Abbreviation TMEM119
Uniprot No. Q4V9L6
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM119 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 107
Range % 103-110
1:2 Average % 88
Range % 84-92
1:4 Average % 107
Range % 103-110
1:8 Average % 90
Range % 87-93
Recovery
The recovery of human TMEM119 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 100-108
EDTA plasma (n=4) 88 84-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 1.805 1.831 1.818 1.691
2000 1.501 1.600 1.551 1.424
1000 1.282 1.198 1.240 1.113
500 1.003 1.068 1.036 0.909
250 0.704 0.712 0.708 0.581
125 0.471 0.498 0.485 0.358
62.5 0.356 0.362 0.359 0.232
0 0.126 0.128 0.127  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts
Gene References into Functions
  1. TMEM119 expression in osteosarcoma tissues is positively correlated with cell cycle, apoptosis, metastasis and TGF-beta signaling. PMID: 28496199
  2. TMEM119 serves as a reliable microglial marker that discriminates resident microglia from blood-derived macrophages in the human brain PMID: 26250788
  3. qPCR showed that TMEM119 expression was highly enriched in temporal lobe CD11b+ cells over unpurified whole brain, and barely detectable in peripheral blood leukocytes. PMID: 26884166
Subcellular Location Cell membrane, Single-pass type I membrane protein, Cytoplasm, Endoplasmic reticulum membrane
Tissue Specificity Expressed in brain microglia (at protein level). Elevated expression levels seen in the brain of patients with Alzheimer disease. Expressed by osteoblast-like cells in bone tissues and follicular dendritic cells in lymphoid tissues.
Database Links

HGNC: 27884

KEGG: hsa:338773

UniGene: Hs.449718

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