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Human Transmembrane protein 119(TMEM119) ELISA kit

Human Transmembrane protein 119(TMEM119) ELISA kit

Protocol

Code	CSB-EL023686HU
Size	96T,5×96T,10×96T
Trial Size
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Target Background

Product Details

Target Name

transmembrane protein 119

Alternative Names

TMEM119 ELISA Kit; PSEC0199 ELISA Kit; UNQ731/PRO1415 ELISA Kit; Transmembrane protein 119 ELISA Kit; Osteoblast induction factor ELISA Kit; OBIF ELISA Kit

Abbreviation

TMEM119

Uniprot No.

Q4V9L6

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM119 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	103-110
1:2	Average %	88
	Range %	84-92
1:4	Average %	107
	Range %	103-110
1:8	Average %	90
	Range %	87-93

Recovery

The recovery of human TMEM119 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	88	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	1.805	1.831	1.818	1.691
2000	1.501	1.600	1.551	1.424
1000	1.282	1.198	1.240	1.113
500	1.003	1.068	1.036	0.909
250	0.704	0.712	0.708	0.581
125	0.471	0.498	0.485	0.358
62.5	0.356	0.362	0.359	0.232
0	0.126	0.128	0.127

ELISA Data Analysis

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Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Background

Function (From Uniprot)	Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts. May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation.
Gene References into Functions	TMEM119 expression in osteosarcoma tissues is positively correlated with cell cycle, apoptosis, metastasis and TGF-beta signaling. PMID: 28496199 TMEM119 serves as a reliable microglial marker that discriminates resident microglia from blood-derived macrophages in the human brain PMID: 26250788 qPCR showed that TMEM119 expression was highly enriched in temporal lobe CD11b+ cells over unpurified whole brain, and barely detectable in peripheral blood leukocytes. PMID: 26884166
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Cytoplasm. Endoplasmic reticulum membrane.
Tissue Specificity	Expressed in brain microglia (at protein level). Elevated expression levels seen in the brain of patients with Alzheimer disease. Expressed by osteoblast-like cells in bone tissues and follicular dendritic cells in lymphoid tissues.
Database Links	HGNC: 27884 KEGG: hsa:338773 UniGene: Hs.449718

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Human Transmembrane protein 119(TMEM119) ELISA kit

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