Human Tumor necrosis factor receptor superfamily member 25(TNFRSF25) ELISA kit

Code CSB-EL023980HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q93038
Lead Time 5-7 working days
Abbreviation TNFRSF25
Protein Biological Process 1 Apoptosis/Autophagy
Target Name tumor necrosis factor receptor superfamily, member 25
Alias RP4-650H14.2, APO-3, DDR3, DR3, LARD, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR, apoptosis inducing receptor|apoptosis-mediating receptor|death domain receptor 3 soluble form|death receptor beta|lymphocyt
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates
Detection Range 0.24 ng/mL-15 ng/mL
Sensitivity 0.06 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.
HGNC 11910
RGD 1598206
MGI 1934667
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %85-93
1:2Average %94
Range %91-99
1:4Average %84
Range %81-89
1:8Average %90
Range %87-93
Recovery
The recovery of human TNFRSF25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8380-86
EDTA plasma (n=4)9188-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
152.464 2.498 2.481 2.321
7.51.696 1.668 1.682 1.522
3.751.101 1.135 1.118 0.958
1.880.712 0.732 0.722 0.562
0.940.513 0.536 0.525 0.365
0.470.398 0.387 0.393 0.233
0.240.297 0.276 0.287 0.127
00.161 0.159 0.160
References
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Function Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.
Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 9: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 11: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 3: Secreted, SUBCELLULAR LOCATION: Isoform 4: Secreted, SUBCELLULAR LOCATION: Isoform 5: Secreted, SUBCELLULAR LOCATION: Isoform 6: Secreted, SUBCELLULAR LOCATION: Isoform 7: Secreted, SUBCELLULAR LOCATION: Isoform 8: Secreted, SUBCELLULAR LOCATION: Isoform 10: Secreted, SUBCELLULAR LOCATION: Isoform 12: Secreted
Tissue Specificity Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.
Database Links

HGNC: 11910

OMIM: 603366

KEGG: hsa:8718

UniGene: Hs.462529

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