Human Tumor necrosis factor receptor superfamily member 25(TNFRSF25) ELISA kit

Code CSB-EL023980HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
tumor necrosis factor receptor superfamily, member 25
Alternative Names
Apo 3 ELISA Kit; Apo-3 ELISA Kit; Apo3 ELISA Kit; Apoptosis inducing receptor AIR ELISA Kit; Apoptosis inducing receptor ELISA Kit; Apoptosis mediating receptor ELISA Kit; Apoptosis mediating receptor DR 3 ELISA Kit; Apoptosis mediating receptor DR3 ELISA Kit; Apoptosis mediating receptor TRAMP ELISA Kit; Apoptosis-inducing receptor AIR ELISA Kit; Apoptosis-mediating receptor DR3 ELISA Kit; Apoptosis-mediating receptor TRAMP ELISA Kit; DDR 3 ELISA Kit; DDR3 ELISA Kit; Death domain receptor 3 ELISA Kit; Death domain receptor 3 soluble form ELISA Kit; Death receptor 3 ELISA Kit; Death receptor beta ELISA Kit; DR 3 ELISA Kit; DR3 ELISA Kit; LARD ELISA Kit; Lymphocyte associated receptor of death ELISA Kit; Lymphocyte-associated receptor of death ELISA Kit; Protein WSL ELISA Kit; Protein WSL-1 ELISA Kit; TNF receptor superfamily member 25 ELISA Kit; TNFR25 ELISA Kit; TNFRSF 12 ELISA Kit; TNFRSF 25 ELISA Kit; TNFRSF12 ELISA Kit; TNFRSF12, formerly ELISA Kit; TNFRSF25 ELISA Kit; TNR25_HUMAN ELISA Kit; TR 3 ELISA Kit; TR3 ELISA Kit; TRAMP ELISA Kit; Translocating chain association membrane protein ELISA Kit; Tumor necrosis factor receptor superfamily member 12 ELISA Kit; Tumor necrosis factor receptor superfamily member 25 ELISA Kit; Tumor necrosis factor receptor superfamily, member 12 (translocating chain association membrane protein) ELISA Kit; Tumor necrosis factor receptor superfamily, member 12, formerly ELISA Kit; WSL 1 ELISA Kit; WSL ELISA Kit; WSL LR ELISA Kit; WSL protein ELISA Kit; WSL1 ELISA Kit; WSL1 protein ELISA Kit; WSLLR ELISA Kit
Abbreviation
TNFRSF25
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.24 ng/mL-15 ng/mL
Sensitivity
0.06 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %85-93
1:2Average %94
Range %91-99
1:4Average %84
Range %81-89
1:8Average %90
Range %87-93
Recovery
The recovery of human TNFRSF25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8380-86
EDTA plasma (n=4)9188-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
152.464 2.498 2.481 2.321
7.51.696 1.668 1.682 1.522
3.751.101 1.135 1.118 0.958
1.880.712 0.732 0.722 0.562
0.940.513 0.536 0.525 0.365
0.470.398 0.387 0.393 0.233
0.240.297 0.276 0.287 0.127
00.161 0.159 0.160
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TNFRSF25 ELISA Kit was designed for the quantitative measurement of Human TNFRSF25 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.24 ng/mL-15 ng/mL and the sensitivity is 0.06 ng/mL.

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Target Background

Function
(From Uniprot)
Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.
Gene References into Functions
  1. Hypermethylation of the proapoptotic genes BCL2 L11 and TNFRSF25 is observed in pleomorphic adenoma of the salivary glands. However, this phenomenon did not impact mRNA transcription. PMID: 28941993
  2. the DR3/TL1A pathway directly enhances human OC formation and resorptive activity, controlling expression and activation of CCL3 and MMP-9. PMID: 28062298
  3. Untreated children with IBD have higher percentage of DR3(+) PBMCs. PMID: 27001939
  4. In addition to apoptosis, DR3 can robustly trigger necroptotic cell death and provide evidence for TL1A-induced, DR3-mediated necrosome assembly. DR3-mediated necroptosis critically depends on receptor-interacting protein 1 (RIP1) and RIP3, the core components of the necroptotic machinery, which activate the pseudo-kinase mixed lineage kinase domain-like, the prototypic downstream effector molecule of necroptosis. PMID: 27592300
  5. Data suggest that human regulatory T-lymphocytes express DR3 and demonstrate DR3/TL1A-mediated activation of signaling via MAP kinases and NFkappaB. (DR3 = death receptor 3; TL1A/TNFSF15 = tumor necrosis factor [ligand] superfamily, member 15) PMID: 28337757
  6. These results raise the possibility for involvement of TL1A/DR3/DR3-mediated mechanisms in epithelial-mesenchymal interactions and the development of inflammation-induced intestinal fibrosis in Crohn's disease. PMID: 27665176
  7. Activation of DR3 is accompanied by inhibition of apoptosis of naive T-lymphocytes in children with acute infectious mononucleosis. PMID: 27682848
  8. Biologics beyond TNF-alpha inhibitors and the effect of targeting the homologues TL1A-DR3 pathway in chronic inflammatory disorders. PMID: 26810853
  9. Higher DR3 levels were associated with early stage chronic lymphocytic leukemia. PMID: 26393680
  10. DR3 is efficiently activated by soluble TL1A trimers. PMID: 26509650
  11. These data identify new roles for DR3 in regulating OB-dependent bone mineral apposition. PMID: 26065008
  12. Human memory IL-18Ralpha and DR3 CD4+ T cells may contribute to antigen-independent innate responses at barrier surfaces. PMID: 25269704
  13. DR3 is expressed by IL-22-producing human group 3 innate lymphoid cells (ILC3s). PMID: 26046454
  14. These results suggested that TL1A could promote Th17 differentiation in rheumatoid arthritis via the activation of RORc, and this effect may be mediated by the binding of TL1A with DR3. PMID: 24832108
  15. Silencing of the DR3 gene affect levels of apoptosis antigen3 ligand in cells. PMID: 25370568
  16. DR3 is expressed in some interstitial vascular endothelial cells in human kidney in situ. These EC also respond to TL1A by activating NF-kappaB. Very low levels of DR3 are seen on the cell surface of HUVEC, which do not respond to TL1A. PMID: 25399326
  17. The changes in frequency of occurrence of spliced variants of DR3/LARD mRNA were directed towards modulation of apoptosis and restraint of antiviral immune response. PMID: 25929035
  18. Distinctions in occurrence of spectrums of DR3/LARD mRNA at healthy volunteers and colon cancer patients can define a different susceptibility of immunocompetent and tumor cells for apoptosis PMID: 25509355
  19. DR-3 drives early cartilage destruction in the antigen-induced model of inflammatory arthritis through the release of CXCL1, maximizing neutrophil recruitment to the joint and leading to enhanced local production of cartilage-destroying enzymes. PMID: 25044706
  20. Suggest tectochrysin leads to apoptotic cell death in NSCLC cells through activation of DR3 and Fas expression via inhibition of STAT3 phosphorylation. PMID: 25083589
  21. TRAMP mice fed with 3'-diindolylmethane-supplemented diet show much lower incidence of tumorigenesis and metastasis than the untreated control group. PMID: 23658110
  22. Collectively, these data suggest a complex role for DR3 in breast cancer development and progression PMID: 23443464
  23. Both TNFRSF25 and TNFRSF4 independently and additively costimulate vaccine-induced CD8+ T cell proliferation following both primary and secondary antigen challenge. PMID: 22956587
  24. Protein expression of tumour necrosis factor (TNF)-like ligand 1A (TL1A) and death-domain receptor (DR)3 is upregulated in the aged bladder tissues. PMID: 22641456
  25. Investigated further the mechanisms by which the E-selectin-activated pathways downstream of DR3 confer a survival advantage to colon cancer cells. PMID: 21722370
  26. It was shown that IL-32 enhanced the cytotoxic effect of natural killer cells on protate cancer cells through activation of DR3 and caspase-3. PMID: 22043900
  27. in active psoriasis, we observed abundant immunostaining for TL1A and significant upregulation of its receptors DR3 and DcR3 PMID: 21672030
  28. role of TNFRSF25:TNFSF15 in disease and health PMID: 21153333
  29. critically involved in the pathogenesis of rheumatoid arthritis PMID: 20125169
  30. These data confirm that silencer of death domains (SODD) and death receptor 3 (DR3) are expressed in a regulated manner during renal transplant rejection, and identify DR3 as a potential inducible mediator of tubular inflammation and injury. PMID: 12875962
  31. TL1A-induced NF-kappaB activation and c-IAP2 production prevent DR3-mediated apoptosis PMID: 12882979
  32. Death receptor 3 gene duplication is associated with rheumatoid arthritis PMID: 15241467
  33. TL1A and DR3 is involved in atherosclerosis via the induction of pro-inflammatory cytokines/chemokines PMID: 15760679
  34. VEGI gene expression is subject to regulation by inflammatory cytokines. VEGI is also able to regulate the expression of several important genes involved in angiogenesis. PMID: 16517446
  35. Results suggest that death receptor-3 activation can mediate apoptosis in osteoblasts, although its activity is highly restricted by its soluble ligand-binding isoform and possibly also by alternate survival signals. PMID: 16986165
  36. These results suggest that caspase-10, DR-3 and IGFBP-3 are involved in apoptosis in the preeclamptic placenta. PMID: 17085968
  37. TNFR25/TL1A pair provides an early signal for cytokine production in the lung, and therefore may be a drug target in attempts to attenuate lung inflammation in asthmatics. PMID: 18411341
  38. HLA-B8 and DR3 haplotype is associated with graft failure after renal transplantation in patients with underlying immunoglobulin A nephropathy PMID: 19674013

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Subcellular Location
[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 9]: Cell membrane; Single-pass type I membrane protein.; [Isoform 11]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.; [Isoform 7]: Secreted.; [Isoform 8]: Secreted.; [Isoform 10]: Secreted.; [Isoform 12]: Secreted.
Tissue Specificity
Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.
Database Links

HGNC: 11910

OMIM: 603366

KEGG: hsa:8718

UniGene: Hs.462529

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