Human Urocortin-3(UCN3) ELISA kit

Code CSB-EL025553HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
urocortin 3 (stresscopin)
Alternative Names
UCN3 ELISA kit; SPCUrocortin-3 ELISA kit; Stresscopin ELISA kit; Urocortin III ELISA kit; Ucn III ELISA kit
Abbreviation
UCN3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human UCN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %86
Range %83-89
1:2Average %101
Range %98-104
1:4Average %105
Range %101-109
1:8Average %90
Range %85-96
Recovery
The recovery of human UCN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8481-89
EDTA plasma (n=4)9996-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.604 2.638 2.621 2.416
2002.284 2.114 2.199 1.994
1001.555 1.598 1.577 1.372
500.977 0.935 0.956 0.751
250.551 0.587 0.569 0.364
12.50.405 0.432 0.419 0.214
6.250.304 0.318 0.311 0.106
00.204 0.206 0.205
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human UCN3 ELISA Kit was designed for the quantitative measurement of Human UCN3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

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Target Background

Function
(From Uniprot)
Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.
Gene References into Functions
  1. Antiamnesic properties of analogs and mimetics of the tripeptide human urocortin 3 PMID: 27262310
  2. In summary, cardiac expression of CRFR1, CRF, and Ucn3 genes is elevated in heart failure and may contribute to the activation of the CRF/Ucn system in these patients. PMID: 27754786
  3. circulating levels elevated and positively correlated with C-reactive protein in polycystic ovary syndrome PMID: 26488073
  4. results show that activation of CRH receptors by CRH ligands stimulates VEGF-A expression in intestinal epithelial cells through the cAMP/CREB pathway PMID: 26350463
  5. The paracrine actions of Ucn3 activate a negative feedback loop that promotes somatostatin release to ensure the timely reduction of insulin secretion upon normalization of plasma glucose. PMID: 26076035
  6. The molecular modifications of urocortin 3[36-38] led to an improved understanding of the relationship between molecular structure and biological activity of this peptide, could be further tested for possible clinical treatment of depression PMID: 25304878
  7. Data suggest that UCN3 expression can serve as a late stage maturation/differentiation marker in beta-cells during pancreogenesis. [REVIEW] PMID: 25148370
  8. SCP inhibits a subpopulation of PVN neurons, especially OTergic magnocellular neurons, by enhancing the activity of GIRK channels via CRF-R2 PMID: 23349753
  9. study showed that Ucn2 and Ucn3 differentially regulate the LPS-induced TNF-alpha and IL-10 expression and secretion in trophoblast explants acting through CRH-R2. Ucn3 has an anti-inflammatory effect. PMID: 22000474
  10. CRH-R1:CRH-R2 ratio varied according to fat-depot type; whereas CRH-R1 expression was higher in sc fat than in visceral fat, the opposite was true for CRH-R2. PMID: 14764822
  11. Expression of Ucn III/SCP in the human heart and kidney as well as brain and pituitary tissues and its presence in plasma and urine. Ucn III/SCP may therefore regulate the cardiac and renal function as a local factor and a circulating hormone. PMID: 15070962
  12. Ucn 3 plays some physiological or pathological roles in the modulation of gastrointestinal functions during stressful conditions. PMID: 15949638
  13. Ucn3 is produced in the normal adrenal gland and in adrenal tumors (both adrenocortical tumors and pheochromocytomas) and acts as an autocrine or paracrine regulator in both the normal and cancerous adrenal gland tissue. PMID: 16095756
  14. the SCP/CRHR2 system is present in human ovaries and treatment with SCP/Ucn3 inhibits progesterone production by cultured granulosa-lutein cells through interaction with CRHR2 PMID: 19351656

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Subcellular Location
Secreted.
Protein Families
Sauvagine/corticotropin-releasing factor/urotensin I family
Database Links

HGNC: 17781

OMIM: 605901

KEGG: hsa:114131

STRING: 9606.ENSP00000369798

UniGene: Hs.511775

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