Human Vasohibin-1(VASH1) ELISA kit

Code CSB-EL025794HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q7L8A9
Lead Time 5-7 working days
Abbreviation VASH1
Protein Biological Process 1 Cell Cycle
Target Name vasohibin 1
Alias KIAA1036,
Species Homo sapiens (Human)
Protein Biological Process 3 Cell cycle
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 19964
RGD 1564082
MGI 2442543
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VASH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %96
Range %89-102
1:2Average %94
Range %88-106
1:4Average %90
Range %82-100
1:8Average %95
Range %86-101
Recovery
The recovery of human VASH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-100
EDTA plasma (n=4)9592-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.466 2.343 2.405 2.280
20001.525 1.588 1.557 1.432
10000.938 0.929 0.934 0.809
5000.550 0.562 0.556 0.431
2500.356 0.373 0.365 0.240
1250.262 0.265 0.264 0.139
62.50.180 0.175 0.178 0.053
00.123 0.127 0.125
References
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Function Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.
Subcellular Location Secreted
Protein Families Vasohibin family
Tissue Specificity Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.
Database Links

HGNC: 19964

OMIM: 609011

KEGG: hsa:22846

STRING: 9606.ENSP00000167106

UniGene: Hs.525479

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