Human a proliferation inducing ligand(APRIL)ELISA Kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

tumor necrosis factor (ligand) superfamily, member 13

Associated names

A proliferation inducing ligand ELISA Kit; A proliferation-inducing ligand ELISA Kit; APRIL ELISA Kit; CD256 ELISA Kit; TALL-2 ELISA Kit; TALL2 ELISA Kit; TNF related death ligand ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 2 ELISA Kit; TNF-related death ligand 1 ELISA Kit; TNF13_HUMAN ELISA Kit; TNFSF13 ELISA Kit; TRDL-1 ELISA Kit; TRDL1 ELISA Kit; Tumor necrosis factor (ligand) superfamily; member 13 ELISA Kit; Tumor necrosis factor ligand superfamily member 13 ELISA Kit; Tumor necrosis factor like protein ZTNF2 ELISA Kit; Tumor necrosis factor related death ligand 1 ELISA Kit; UNQ383/PRO715 ELISA Kit; ZTNF2 ELISA Kit

Abbreviation

TNFSF13

Uniprot No.

O75888

Species

Homo sapiens (Human)

Sample Types

serum, plasma, saliva, cell culture supernates

Detection Range

7.8 pg/mL-500 pg/mL.

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APRIL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2	Average %	92
	Range %	87-99
1:4	Average %	100
	Range %	95-109
1:8	Average %	86
	Range %	82-90
1:16	Average %	93
	Range %	86-96

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
500	3.682	3.464	3.573	3.486
250	2.184	2.120	2.152	2.065
125	1.372	1.398	1.385	1.298
62.5	0.794	0.842	0.818	0.731
31.2	0.541	0.565	0.553	0.466
15.6	0.386	0.369	0.377	0.290
7.8	0.200	0.188	0.194	0.107
0	0.086	0.088	0.087

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This ELISA kit provides quantitative measurement of human TNFSF13 (APRIL/A Proliferation-Inducing Ligand) in biological samples, supporting research into B cell biology, humoral immunity, and inflammatory disease. Because APRIL is a secreted cytokine whose circulating levels shift modestly between health and disease — and may be present at low concentrations in mucosal or in vitro systems — precise detection across a defined dynamic window is essential.

Detection Range: The 7.8–500 pg/mL range aligns with reported serum APRIL concentrations in healthy individuals and the elevated levels observed in autoimmune and lymphoproliferative conditions, minimizing the need for extensive sample dilution while capturing disease-relevant shifts.

Sensitivity: At 3.9 pg/mL, the kit detects APRIL in low-abundance matrices such as saliva and conditioned media from stimulated B cells or dendritic cell co-cultures, where secreted levels may fall well below circulating concentrations.

Sample Compatibility: Validation across serum, plasma, saliva, and cell culture supernatants enables researchers to correlate systemic APRIL levels with mucosal immunity or dissect APRIL secretion kinetics in vitro within a single assay platform.

Assay Efficiency: Requiring only 50–100 µL per well and completable in 1–5 hours, the kit accommodates limited-volume clinical specimens and time-sensitive experiments involving primary human B cell or myeloid cell cultures.

This kit is directly applicable to studies of IgA nephropathy, systemic lupus erythematosus, and B cell lymphoma tumor microenvironments.

Code	CSB-E15012h-IS
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.