Human alpha-L-fucosidase,AFU ELISA Kit

Code CSB-E08348h
Size 96T,5×96T,10×96T
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Product Details

Target Name
fucosidase, alpha-L- 2, plasma
Alternative Names
Alpha L fucosidase 2 ELISA Kit; Alpha L fucosidase fucohydrolase 2 ELISA Kit; Alpha-L-fucosidase 2 ELISA Kit; Alpha-L-fucoside fucohydrolase 2 ELISA Kit; dJ20N2.5 ELISA Kit; FUCA 2 ELISA Kit; FUCA2 ELISA Kit; FUCO2 ELISA Kit; FUCO2_HUMAN ELISA Kit; Fucosidase alpha L 2 plasma ELISA Kit; Fucosidase; alpha-L; plasma ELISA Kit; MGC1314 ELISA Kit; Plasma alpha L fucosidase ELISA Kit; Plasma alpha-L-fucosidase ELISA Kit; RP1 20N2.5 ELISA Kit
Abbreviation
FUCA2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 μU/mL-2000 μU/mL
Sensitivity
7.8 μU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AFU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %88
Range %81-95
1:40Average %91
Range %86-97
1:80Average %97
Range %91-102
1:160Average %102
Range %97-107
Recovery
The recovery of human AFU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10395-110
EDTA plasma (n=4)9385-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
µU/mlOD1OD2AverageCorrected
20002.720 2.546 2.633 2.546
10002.235 2.200 2.218 2.131
5001.554 1.633 1.594 1.507
2500.821 0.845 0.833 0.746
1250.471 0.445 0.458 0.371
62.50.311 0.302 0.307 0.220
31.250.185 0.188 0.187 0.100
00.084 0.090 0.087  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human FUCA2 ELISA Kit was designed for the quantitative measurement of Human FUCA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 μU/mL-2000 μU/mL and the sensitivity is 7.8 μU/mL.

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Target Background

Function
(From Uniprot)
Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
Gene References into Functions
  1. Genetic polymorphisms within the FUCA2 and IL18 gene regions are also associated with diastolic function in SCD, likely by affecting expression levels of the genes PMID: 27636371
  2. serum alpha-L-fucosidase was identified as a useful marker for close monitoring of patients during post-treatment follow-up PMID: 18521898
  3. Findings not only demonstrate an important connection between FUCA2 and the adhesion, growth, and pathogenicity of H. pylori, but also support the idea that FUCA2 is a potential target for diagnosis and therapy of H. pylori-related diseases. PMID: 19666478
Subcellular Location
Secreted.
Protein Families
Glycosyl hydrolase 29 family
Database Links

HGNC: 4008

OMIM: 136820

KEGG: hsa:2519

STRING: 9606.ENSP00000002165

UniGene: Hs.17680

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