Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

FUCA2

Uniprot No.

Q9BTY2

Research Area

Metabolism

Alternative Names

Alpha L fucosidase 2; Alpha L fucosidase fucohydrolase 2; Alpha-L-fucosidase 2; Alpha-L-fucoside fucohydrolase 2; dJ20N2.5; FUCA 2; FUCA2; FUCO2; FUCO2_HUMAN; Fucosidase alpha L 2 plasma; Fucosidase; alpha-L; plasma; MGC1314; Plasma alpha L fucosidase; Plasma alpha-L-fucosidase; RP1 20N2.5

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

29-465aa

Target Protein Sequence

HSATRFDPTWESLDARQLPAWFDQAKFGIFIHWGVFSVPSFGSEWFWWYWQKEKIPKYVEFMKDNYPPSFKYEDFGPLFTAKFFNANQWADIFQASGAKYIVLTSKHHEGFTLWGSEYSWNWNAIDEGPKRDIVKELEVAIRNRTDLRFGLYYSLFEWFHPLFLEDESSSFHKRQFPVSKTLPELYELVNNYQPEVLWSDGDGGAPDQYWNSTGFLAWLYNESPVRGTVVTNDRWGAGSICKHGGFYTCSDRYNPGHLLPHKWENCMTIDKLSWGYRREAGISDYLTIEELVKQLVETVSCGGNLLMNIGPTLDGTISVVFEERLRQMGSWLKVNGEAIYETHTWRSQNDTVTPDVWYTSKPKEKLVYAIFLKWPTSGQLFLGHPKAILGATEVKLLGHGQPLNWISLEQNGIMVELPQLTIHQMPCKWGWALALTN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

77.7 kDa

Protein Length

Partial

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human FUCA2 covers amino acids 29-465. The theoretical molecular weight of the FUCA2 protein is 77.7 kDa. This protein is generated in a e.coli-based system. The N-terminal GST tag was fused into the coding gene segment of FUCA2, making it easier to detect and purify the FUCA2 recombinant protein in the later stages of expression and purification.

Human plasma alpha-L-fucosidase, encoded by the FUCA2 gene, is an enzyme involved in the hydrolysis of alpha-L-fucosidic linkages in various glycoconjugates. This lysosomal enzyme plays a crucial role in the degradation of complex carbohydrates, including glycoproteins and glycolipids containing terminal fucose residues, contributing to cellular homeostasis. Deficiencies in human FUCA2 are associated with fucosidosis, a lysosomal storage disorder characterized by the accumulation of fucose-containing substances. The study of FUCA2 contributes to understanding lysosomal function, glycoprotein metabolism, and the pathological mechanisms underlying fucosidosis, providing insights into potential therapeutic strategies for lysosomal storage disorders.

Target Background

Function

Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.

Gene References into Functions

Genetic polymorphisms within the FUCA2 and IL18 gene regions are also associated with diastolic function in SCD, likely by affecting expression levels of the genes PMID: 27636371
serum alpha-L-fucosidase was identified as a useful marker for close monitoring of patients during post-treatment follow-up PMID: 18521898
Findings not only demonstrate an important connection between FUCA2 and the adhesion, growth, and pathogenicity of H. pylori, but also support the idea that FUCA2 is a potential target for diagnosis and therapy of H. pylori-related diseases. PMID: 19666478

Subcellular Location

Secreted.

Protein Families

Glycosyl hydrolase 29 family

Database Links

HGNC: 4008

OMIM: 136820

KEGG: hsa:2519

STRING: 9606.ENSP00000002165

UniGene: Hs.17680

Code	CSB-EP863122HU1
Abbreviation	Recombinant Human FUCA2 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Plasma alpha-L-fucosidase (FUCA2), partial

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