Human angiotensin Ⅱ receptor 1,ANGⅡR-1 ELISA Kit

Instructions
Code CSB-E11239h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name angiotensin II receptor, type 1
Alternative Names AG2S ELISA Kit; Agtr 1 ELISA Kit; Agtr1 ELISA Kit; AGTR1_HUMAN ELISA Kit; Agtr1a ELISA Kit; AGTR1B ELISA Kit; Ang II ELISA Kit; Angiotensin II receptor type 1 ELISA Kit; Angiotensin II type-1 receptor ELISA Kit; Angiotensin receptor 1 ELISA Kit; Angiotensin receptor 1B ELISA Kit; AT 1B ELISA Kit; AT 1r ELISA Kit; AT1 ELISA Kit; At1a ELISA Kit; AT1AR ELISA Kit; AT1B ELISA Kit; AT1BR ELISA Kit; AT1R ELISA Kit; AT2R1 ELISA Kit; AT2R1A ELISA Kit; AT2R1B ELISA Kit; HAT1R ELISA Kit; Type 1 angiotensin II receptor ELISA Kit; Type 1B angiotensin II receptor ELISA Kit; Type-1 angiotensin II receptor ELISA Kit
Abbreviation AGTR1
Uniprot No. P30556
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.72 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANGⅡR-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 81-98  
1:2 Average % 96  
Range % 93-105  
1:4 Average % 94  
Range % 87-98  
1:8 Average % 103  
Range % 96-110  
Recovery
The recovery of human ANGⅡR-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-96  
EDTA plasma (n=4) 99 90-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
3000 2.354 2.254 2.304 2.153  
1500 1.712 1.612 1.662 1.511  
750 1.131 1.081 1.106 0.955  
375 0.566 0.556 0.561 0.410  
187.5 0.338 0.328 0.333 0.182  
93.75 0.277 0.267 0.272 0.121  
46.88 0.209 0.205 0.207 0.056  
0 0.152 0.150 0.151    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Gene References into Functions
  1. Intracrine action of angiotensin II in mesangial cells: subcellular distribution of angiotensin II receptor subtypes AT1 and AT2. PMID: 29455433
  2. Meta-analysis suggests that AGTR1 A1166C polymorphism may increase the susceptibility to diabetic nephropathy. PMID: 30313019
  3. Results may indicate that the choice of diet can undermine the potential genetic risk of the AT1R 1166A>C polymorphism for MetS, and persons who are variant carriers may spontaneously choose the Mediterranean diet. PMID: 30205174
  4. The research reveals that among patients with essential hypertension treated with hypotensive drugs there are certain relationships between the rs5182 and rs5186 polymorphisms of the AGTR1 gene, as well as between the rs1799998 polymorphism of the CYP11B2 gene and the volume of the carotid bodies. PMID: 29627490
  5. The protective effects of short-term hypoxia (STH) are associated with the upregulation of MasR to inhibit AT1 R signaling. STH could be a potential therapeutic strategy for cardiac diseases in hypertensive patients. PMID: 29052864
  6. A high level of ART1 antibodies was a risk factor for allograft function in kidney transplant recipients. PMID: 29507275
  7. we found an interaction between ACE2 and AGTR1 in structuralatrial fibrillation patients in a Chinese Han population PMID: 29441892
  8. AGTR1 DNA hypomethylation is a risk factor for the development of hypertension. Furthermore, there is a correlation between smoking, diet, and AGTR1 DNA methylation levels. PMID: 28376480
  9. Chinese Han persons with C allele of rs9817428 in PPARG gene were related to the increased risk of hypertension. PMID: 29266977
  10. Association of AGTR1 single nucleotide polymorphism rs275645 and pre-eclampsia in Chinese population. PMID: 27910864
  11. although the present study did not find any association between AGTR1 A1166C polymorphism and the risk of systemic lupus erythematosus, the presence of this polymorphism was associated with higher levels of malondialdehyde and higher concentration of neopterin PMID: 28530466
  12. We conclude that the crosstalk between angiotensin AT1 receptor and insulin receptor signaling shows a high degree of specificity, and involves Galphaq protein, and activation of distinct kinases. Thus, the BRET(2) technique can be used as a platform for studying molecular mechanisms of crosstalk between insulin receptor and 7TM receptors. PMID: 28854843
  13. The AGTR1-mediated cell apoptosis was suppressed by overexpressing miR-1248 and inhibited protein translation of AGTR1 by miR-1248, through binding to the 3'-UTR of the AGTR1 mRNA. PMID: 29482191
  14. The effects ofgenetic polymorphisms and diet on the risk of metabolic disorders predisposing to cardiovascular diseases among Malay, Chinese, and South Asian persons are reported. PMID: 28792482
  15. AGTR1 gene, related to renin-angiotensin system, might play momentous roles in the initiation and development of consecutive Trauma-Induced Sepsis. PMID: 29642183
  16. The genotypes of REN, AT1R and AT2R were not associated with the development of preeclampsia in South African Black women. PMID: 28641212
  17. AC-CC genotype of AT1R and noise were the risky factors of EH. These factors also interacted with each other. PMID: 28157766
  18. the A1166C polymorphism in the AGTR1 gene may not be a risk factor for breast cancer (Meta-Analysis) PMID: 26634463
  19. High levels of AT1R is associated with angiogenesis in bladder cancer. PMID: 28599664
  20. High angiotensin II type 1 receptor antibodies are associated with graft rejection in renal transplantation. PMID: 26546592
  21. No statistically significant data on the correlation between the AGTR1 A/C polymorphism and the development of Atrial Fibrillation were obtained in any patient subgroup of a Siberian population. PMID: 29039830
  22. Letter/Case Report: refractory vascular rejection in a hand transplant recipient in the presence of AGTR1 antibodies. PMID: 28767535
  23. These findings suggest that AT1R overexpression is an independent adverse prognosticator for patients with esophageal squamous cell carcinoma PMID: 27564102
  24. As previously found for rs1799752 in ACE, rs5186 in AGTR1 was associated with dementia at baseline. These associations became substantially weaker, or disappeared, when dementia onset to 2012 was included. PMID: 27639288
  25. Study identified the first significant associations between SNPs rs275651, rs275652 and rs4524238 in AGTR1 gene and high-altitude pulmonary edema susceptibility. PMID: 27732943
  26. There was no association for rs5186 with LVH under any of models. PMID: 28513230
  27. Homocysteine displaces angiotensin II and regulates the conformation of the AT1 receptor. PMID: 29296021
  28. no statistical association with kidney graft failure was found for anti-AT1R antibodies, indicating that these tests may not be the best predictors of graft outcome in living donor renal transplantation. PMID: 28372986
  29. Inhibition of CaV1.2 upregulates AT1R signaling in response to angiotensin II. PMID: 28514967
  30. Single nucleotide polymorphisms of AGTR1 gene are associated with Hypertension. PMID: 27045027
  31. The presence of AGTR1 autoantibodies is an independent predictor for aortic stiffness progression in normotensive subjects. PMID: 29097625
  32. The DEGs, such as AGTR1, CYP3A4 and CYP4A11 may play critical roles in the development of HTN likely via the regulation by hsa-miR-26b-5p and taking part in some pathways. PMID: 27756246
  33. Low shear stress induces the generation of endothelial reactive oxygen species via AT1R/eNOS/NO signaling pathway. PMID: 28518223
  34. Low AGTR1 expression is associated with chemoresistance of osteosarcoma. PMID: 28073349
  35. No relationship was found between the studied polymorphisms (14094 ACE gene, rs1800469 gene TGFbeta1, GNB3 gene rs5443, rs5186 AGTR1 gene) and the occurrence of primary vesicoureteral reflux. PMID: 27988909
  36. Angiotensin II type 1 receptors promote ADAM17-mediated ACE2 shedding in the brain of hypertensive patients. PMID: 28512108
  37. Data suggest that allosteric communication between heterodimeric AT1R and PTGFR is mediated through GNAQ and may also involve proximal phospholipase C but not distal protein kinase C signaling partners; PTGFR activation has negligible effects on AT1R-based conformational biosensors. (AT1R = angiotensin II receptor, type 1; PTGFR = prostaglandin F2alpha receptor; GNAQ = GTP-binding protein G[q] subunit alpha) PMID: 28584054
  38. our results show the pivotal mechanisms of AT1R-induced harmful phenotype of Plasmodium-specific CD8(+) T cells during blood-stage malaria. PMID: 28261571
  39. Ouabain stimulates NKA in renal proximal tubule cells through an angiotensin/AT1R-dependent mechanism and that this pathway contributes to cardiac glycoside associated hypertension. PMID: 27496272
  40. Mutations within transmembrane domains IV, V, VI, and VII had no effect on angiotensin-mediated beta-arrestin1 recruitment; however, they exhibited differential effects on the assembly of AT1R into oligomeric complexes. PMID: 28096461
  41. Our findings provide evidence that induction of APP shedding via Ang II/AT1 receptor stimulation is effected by G protein activation with Gbg subunits playing important roles. PMID: 28102934
  42. highly and selectively expressed in colonic L cells PMID: 27447725
  43. AT1R-Ab and anti-endothelial cell antibodies may identify patients at higher risk for antibody mediated injury, particularly in the presence of HLA-donor specific antibodies. PMID: 27222934
  44. Structure and Function of Cross-class Complexes of G Protein-coupled Secretin and Angiotensin 1a Receptors. PMID: 27330080
  45. studied the clinical significance of the AT1RA1166C receptor polymorphism and its relationship with serum concentration of lipids, oxidant/antioxidant systems and vascular adhesion protein-1(VAP-1) in 100 psoriatic patients and 100 gender- and age-matched unrelated healthy controls from west population of Iran PMID: 26395033
  46. angiotensin II Type 1 receptor genotype may influence the clinical expression of vasculopathy in systemic sclerosis patients. PMID: 27488275
  47. Single nucleotide polymorphisms (SNP) in angiotensin II receptor, type 1 (AGTR1), transcription factor AP-2 beta (TFAP2B), and tumor necrosis factor receptor-associated factor 1 (TRAF1) have been reported to be associated with the incidence of PDA in preterm infants. PMID: 26615960
  48. AGTR1 rs3772622 gene polymorphism was not associated with the risk of developing non-alcoholic fatty liver disease (NAFLD), but could increase the risk of NAFLD patients suffering from coronary artery disease in the Chinese Han population. PMID: 27342049
  49. Results demonstrated that rs1492099, a SNP of AGTR1 gene, could be a potential genetic risk factor of arterio-venous fistula malfunction in male hemodialysis patients. PMID: 27240348
  50. Median total Angiotensin II type 1 receptor IgG concentration was significantly higher in the stable pediatric kidney transplant group as compared to the adult transplant group. For both groups, the transplant group showed higher levels than the control group. PMID: 27251358

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Involvement in disease Renal tubular dysgenesis (RTD)
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Liver, lung, adrenal and adrenocortical adenomas.
Database Links

HGNC: 336

OMIM: 106165

KEGG: hsa:185

STRING: 9606.ENSP00000273430

UniGene: Hs.477887

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