Human angiotensin Ⅱ receptor 1,ANGⅡR-1 ELISA Kit

Code CSB-E11239h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P30556
Lead Time 3-5 working days
Abbreviation AGTR1
Target Name angiotensin II receptor, type 1
Alias AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT1R, AT2R1, AT2R1A, AT2R1B, HAT1R, angiotensin receptor 1|angiotensin receptor 1B|type-1B angiotensin II receptor
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.72 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least five transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants.
HGNC 336
RGD 2070
MGI 87964
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANGⅡR-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 81-98  
1:2 Average % 96  
Range % 93-105  
1:4 Average % 94  
Range % 87-98  
1:8 Average % 103  
Range % 96-110  
Recovery
The recovery of human ANGⅡR-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-96  
EDTA plasma (n=4) 99 90-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
3000 2.354 2.254 2.304 2.153  
1500 1.712 1.612 1.662 1.511  
750 1.131 1.081 1.106 0.955  
375 0.566 0.556 0.561 0.410  
187.5 0.338 0.328 0.333 0.182  
93.75 0.277 0.267 0.272 0.121  
46.88 0.209 0.205 0.207 0.056  
0 0.152 0.150 0.151    
References
Still Have Questions? Leave a Message or Start an on-line Chat
Function Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Involvement in disease Renal tubular dysgenesis (RTD)
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Liver, lung, adrenal and adrenocortical adenomas.
Database Links

HGNC: 336

OMIM: 106165

KEGG: hsa:185

STRING: 9606.ENSP00000273430

UniGene: Hs.477887

Pathway Calcium signaling pathway
Renin-angiotensin system
Vascular smooth muscle contraction
AGE-RAGE signaling pathway in diabetic complications
Apelin signaling pathway
cGMP-PKG signaling pathway
Oxidative Phosphorylation
Phospholipase D signaling pathway

Related Products

AGTR1 Antibodies

AGTR1 Antibodies for Homo sapiens (Human)

AGTR1 Antibodies for Human

AGTR1 Proteins

AGTR1 Proteins for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.