Human apoptosis inducing factor,AIF ELISA Kit

Instructions
Code CSB-E09006h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name apoptosis-inducing factor, mitochondrion-associated, 1
Alternative Names AIFM1 ELISA Kit; AIFM1_HUMAN ELISA Kit; Apoptosis inducing factor 1, mitochondrial ELISA Kit; Apoptosis inducing factor ELISA Kit; Apoptosis inducing factor, mitochondrion associated, 1 ELISA Kit; Apoptosis-inducing factor 1 ELISA Kit; CMTX4 ELISA Kit; COWCK ELISA Kit; COXPD6 ELISA Kit; Harlequin ELISA Kit; Hq ELISA Kit; mAIF ELISA Kit; MGC111425 ELISA Kit; MGC5706 ELISA Kit; mitochondrial ELISA Kit; Neuropathy, axonal motor-sensory, with deafness and mental retardation ELISA Kit; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) ELISA Kit; PDCD 8 ELISA Kit; PDCD8 ELISA Kit; Programmed cell death 8 (apoptosis inducing factor) ELISA Kit; Programmed cell death 8 ELISA Kit; Programmed cell death 8 isoform 1 ELISA Kit; Programmed cell death 8 isoform 2 ELISA Kit; Programmed cell death 8 isoform 3 ELISA Kit; Programmed cell death protein 8 ELISA Kit; Programmed cell death protein 8 mitochondrial ELISA Kit; Programmed cell death protein 8 mitochondrial precursor ELISA Kit; Programmed cell death protein 8 mitochondrial precursor ELISA Kit; Striatal apoptosis inducing factor ELISA Kit
Abbreviation AIFM1
Uniprot No. O95831
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-95
1:2Average %89
Range %85-94
1:4Average %88
Range %82-95
1:8Average %98
Range %92-103
Recovery
The recovery of human AIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9491-98
EDTA plasma (n=4)8780-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.361 2.545 2.453 2.295
7501.806 1.707 1.757 1.599
3751.072 1.154 1.113 0.955
187.50.682 0.673 0.678 0.520
940.455 0.443 0.449 0.291
470.297 0.305 0.301 0.143
23.50.223 0.229 0.226 0.068
00.157 0.158 0.158
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Gene References into Functions
  1. Here, the binding site of AIF(Delta1-121) and AIF(370-394) on CypA has been mapped by NMR spectroscopy and biochemical studies, and a molecular model of the complex has been proposed. The authors show that AIF(370-394) interacts with CypA on the same surface recognized by AIF(Delta1-121) protein and that the region is very close to the CypA catalytic pocket. PMID: 28442737
  2. new form of motor neuropathy expands the phenotypic spectrum of AIFM1 mutations and therefore, the AIFM1 gene should be considered in the diagnosis of hereditary motor neuropathies PMID: 28975462
  3. X-linked hypomyelination with spondylometaphyseal dysplasia associated with mutations in AIFM1 PMID: 28842795
  4. report of a family carrying a novel missense mutation in AIFM1 that exhibits an isolated axonal polyneuropathy with misassembly of mitochondrial complex I and III. PMID: 28888069
  5. we sought to investigate mechanisms mediated by Hsp70 acetylation in relation to apoptotic and autophagic programmed cell death. Upon stress-induced apoptosis, Hsp70 acetylation inhibits apoptotic cell death, mediated by Hsp70 association with apoptotic protease-activating factor (Apaf)-1 and apoptosis-inducing factor (AIF), key modulators of caspase-dependent and -independent apoptotic pathways, respectively PMID: 28627586
  6. Overexpression of full-length AIFM1 suppresses proliferation and induces apoptosis of HepG2 and Hep3B cells. Caspase 3 and DRAM are involved in full-length AIFM1-induced apoptosis in HepG2 and Hep3B cells. PMID: 29501488
  7. Real-time PCR results showed that apoptosis-inducing factor (AIF) and calpain-1 mRNAs were highly expressed in the transitional segmentof the Hirschsprung's disease bowel, whereas autophagy protein 5 (Atg5) was highly expressed in the narrow segment. Western blot results were consistent with mRNA levels, with increased AIF, calpain-1, and Atg5 expressions in the transitional segment compared with the dilated segment. PMID: 28562483
  8. Data revealed that under a nutrient deficient condition, CD317 functions as an anti-apoptotic factor through AIF-mediated caspase and autophagy-independent manner. PMID: 27444183
  9. we report that AIF-independent PARP-1-dependent necrosis constitutes a major mechanism of RPE cell death leading to retinal degeneration in dry age-related macular degeneration . PMID: 28055012
  10. Downregulation of AIF by hypoxia causes oxidative inactivation of the lipid phosphatase activity of phosphatase and tensin homolog on chromosome 10 (PTEN), with ensuing activation of Akt kinase, phosphorylation of the Akt substrate GSK-3beta and activation of WNT/beta-catenin signaling in colon cancer cells. PMID: 27543779
  11. novel pathway for mda-7/IL-24-induced caspase-independent apoptosis in neuroblastoma cells mediated through modulation of AIF, ATM, and gamma-H2AX. PMID: 27197168
  12. Study defines the mechanism whereby AIF is allosterically modulated by charge-complex formation with NADH. Two distinct molecular pathways couple AIF's active site to dimerization and release of its regulatory C-loop insert with implications for AIF allosteric switching in cell survival and death. PMID: 27818101
  13. Whole exome sequencing indicated a single plausible candidate - the p.Asp237Gly variant in apoptosis inducing factor mitochondria associated 1 (AIFM1; chr. Xq26.1), and the p.Asp237Gly segregated with disease in two families. PMID: 27102849
  14. Only a drastic decrease in the expression level or/and redox activity of AIF tends to cause an early and severe neurodegeneration, whereas less pronounced changes in the AIF properties could lead to a broad range of slowly progressive neurological disorders. PMID: 27178839
  15. AIF translocation plays a role in influenza-virus-induced apoptosis. PMID: 27853862
  16. Copper induces the activation of apoptosis through casp3/casp8/casp9/AIF/TP53 signaling pathways. Suggest that apoptosis activation mechanism is dependent on the concentration, time of exposure to Cu and cell type. PMID: 27046389
  17. Estradiol inhibits oxidative stress-induced accumulation of AIF in nucleolus and PARP1-dependent cell death via estrogen receptor alpha in MCF-7 cells. PMID: 25280774
  18. AIF may participate in but not be dominant in the process of retinal pigment epithelium cell apoptosis induced by oxidative stress. PMID: 27453270
  19. Altogether these data indicate that AIF supports the growth and survival of metabolically defined pancreatic cancer cells and that this metabolic function may derive from a novel mechanism so far undocumented in other cancer types. PMID: 27108222
  20. Cdk5-mediated phosphorylation of CHIP negatively regulates its neuroprotective function, thereby contributing to truncated AIF mediated neuronal cell death progression following neurotoxic stimuli. PMID: 26206088
  21. Results identify PTEN as the substrate of AIF oxidoreductase and reveal a novel function for AIF in controlling tumor metastasis. PMID: 26415504
  22. UDA decreased the St-induced changes in mitochondrial and cytosolic AIF level, whereas in Dox-model it affected only the cytosolic AIF content. PMID: 26012840
  23. strongly support a direct relationship between AIF nuclear translocation and radiation induced cell death in hepatocellular carcinoma cell line HepG2 PMID: 26920061
  24. identification of the novel interaction between Trx1 and AIF has provided opportunities to design and develop therapeutically relevant strategies that either promote or prevent this protein-protein interaction for the treatment of different disease PMID: 26119781
  25. AIF bears a HSP-70 binding domain allowing association with HSP-70. PMID: 26165468
  26. Missense mutations in AIFM1 are associated with X-linked recessive auditory neuropathy spectrum disorder. PMID: 25986071
  27. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type). PMID: 26063804
  28. Summarizing what is known today, mutations in AIFM1 are associated with a progressive disorder with myopathy, ataxia and neuropathy. PMID: 25583628
  29. These results suggest that AIF downregulation is a common event in kidney tumor development. AIF loss may lead to decreased STK3 activity, defective apoptosis and malignant transformation PMID: 24992339
  30. Residues Regulating the Reductase Activity of the Human Mitochondrial Apoptosis Inducing Factor PMID: 26237213
  31. Data indicate that poptosis-inducing factor (AIF) controls the mitochondrial import of mitochondrial membrane transport protein CHCHD4. PMID: 26004228
  32. review describes the multistep course of parthanatos and its dependence on PAR signalling and nuclear AIF translocation. PMID: 24684389
  33. Increased trophoblast apoptosis and altered expression levels of syncytin-1, calpain 1, and AIF is observed in preeclamptic placentas. PMID: 24413738
  34. Inhibition of the AIF/CypA complex protects against intrinsic death pathways induced by oxidative stress. PMID: 24434516
  35. both the mitochondrial and apoptotic functions of hAIF are interconnected and coenzyme controlled: a key information in the understanding of the physiological role of AIF in the cellular life and death cycle. PMID: 24914854
  36. The E1B-55K or E4orf3 proteins independently prevented nuclear fragmentation subsequent to release of AIF from the mitochondria, possibly by altering the intracellular distribution of PAR-modified proteins. PMID: 24889240
  37. Data show that buthionine sulfoximine and synergizes with hydroxychavicol in inducing apoptosis of chronic myeloid leukemic (CML) cells through the GSH-ROS-JNK-ERK-iNOS pathway by apoptosis inducing factor (AIF)-dependent manner. PMID: 24040019
  38. Aif seems to have pro-apoptotic and anti-apoptotic roles in the same lineage in different contexts and at different stages PMID: 24434313
  39. Bit1 plays pivotal roles in the development and progression of ESCC, and its biological functions in ESCC may be closely associated with AIF and Bcl-2 levels. PMID: 23955799
  40. Apoptosis-inducing factor and leukocyte elastase inhibitor derived DNase II interact and can cooperate to induce cell death. PMID: 23673989
  41. The diarylheptanoid hirsutenone sensitizes chemoresistant ovarian cancer cells to cisplatin via modulation of apoptosis-inducing factor and X-linked inhibitor of apoptosis. PMID: 24247248
  42. the caspase-independent mitochondrial apoptosis factor, apoptosis-inducing factor (AIF) was upregulated following SPG treatment. These findings indicate that SPG exerts its anticancer effects on RC-58T/h/SA#4 primary prostate cancer cells PMID: 23443329
  43. Calpain activation via Ca(2) flux plays an essential role in eliciting an AIF-mediated, caspase-independent apoptotic pathway in EV71-infected cells. PMID: 23515028
  44. AIF knockdown disrupts function of complex I and therefore increases the superoxide production in mitochondria PMID: 23682427
  45. Taken together our results demonstrate that parthenolide causes in the two cell lines a caspase-independent cell death, which is mediated by AIF(apoptosis-inducing factor ). PMID: 22688575
  46. AIF is an important factor for advanced prostate cancer cells and that through control of energy metabolism and redox balance, the enzymatic activity of AIF is critical for this support. PMID: 23118229
  47. Our findings expand the spectrum of AIF-related disease and provide insight into the effects of AIFM1 mutations. PMID: 23217327
  48. We found AIF to be strongly expressed in the villous trophoblast and that small amounts of AIF were localized to the nucleus of the cells. PMID: 22133578
  49. AIF is imported from the endoplasmic reticulum to the mitochondria via mitochondria-associated membranes and transport vesicles. PMID: 22134679
  50. Different mechanisms preserve translation of programmed cell death 8 and JunB in poliovirus-infected endothelial cells. PMID: 22328780

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Involvement in disease Combined oxidative phosphorylation deficiency 6 (COXPD6); Cowchock syndrome (COWCK); Deafness, X-linked, 5 (DFNX5)
Subcellular Location Mitochondrion intermembrane space, Mitochondrion inner membrane, Cytoplasm, Nucleus, Cytoplasm, perinuclear region, Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit), During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol), AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis, Colocalizes with EIF3G in the nucleus and perinuclear region, SUBCELLULAR LOCATION: Isoform 3: Mitochondrion intermembrane space, Mitochondrion inner membrane
Protein Families FAD-dependent oxidoreductase family
Tissue Specificity Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.
Database Links

HGNC: 8768

OMIM: 300169

KEGG: hsa:9131

STRING: 9606.ENSP00000287295

UniGene: Hs.424932

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