Human calcitonin gene related peptide,CGRP ELISA Kit

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Human calcitonin gene related peptide,CGRP ELISA Kit

Name: Human calcitonin gene related peptide,CGRP ELISA Kit
Brand: CUSABIO
SKU: CSB-E08210h

Citations (9) Protocol

Code	CSB-E08210h
Size	96T,5×96T,10×96T
Trial Size
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Product Details
Citations (9)
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Customer Reviews and Q&A
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Description

This Human CGRP ELISA Kit was designed for the quantitative measurement of Human CGRP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

Target Name

calcitonin gene related peptide,CGRP

Alternative Names

Beta type CGRP ELISA Kit; Beta-CGRP ELISA Kit; Beta-type CGRP ELISA Kit; CALC2 ELISA Kit; Calcb ELISA Kit; CALCB_HUMAN ELISA Kit; Calcitonin gene related peptide 2 ELISA Kit; Calcitonin gene related peptide II ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; Calcitonin related polypeptide; beta ELISA Kit; CGRP II ELISA Kit; CGRP-II ELISA Kit; CGRP2 ELISA Kit; CGRPII ELISA Kit; FLJ30166 ELISA Kit

Abbreviation

CGRP

Uniprot No.

P10092

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

1.56 pg/mL-100 pg/mL

Sensitivity

0.39 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	97
	Range %	92-110
1:8	Average %	90
	Range %	86-98

Recovery

The recovery of human CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.660	2.511	2.586	2.432
50	2.080	2.152	2.116	1.962
25	1.451	1.366	1.409	1.255
12.5	0.795	0.744	0.770	0.616
6.25	0.491	0.523	0.507	0.353
3.12	0.315	0.325	0.320	0.166
1.56	0.248	0.244	0.246	0.092
0	0.157	0.151	0.154

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Network Pharmacology Analysis of Hewei Jiangni Granule for Gastroesophageal Reflux Disease and Experimental Verification of Its Anti-Neurogenic Inflammation Mechanism Y Cheng,Drug design, development and therapy,2022
Increase in Serum Calcitonin Gene-Related Peptide β (CGRPβ) Levels in COVID-19 Patients with Diarrhea: An Underlying Mechanism? G Gárate,Digestive diseases and sciences,2022
Baseline tear fluid CGRP is elevated in active cluster headache patients as long as they have not taken attack abortive medication K Kamm,Cephalalgia,2020
Changes in ET-1, Plasma Neuropeptide Y, and CGRP in Child Patients With Congenital Heart Disease Complicated With Pulmonary Hypertension Before and After Operation Z Liu,Clinical ediatrics,2020
Correlation between CGRP Levels and the Neuropathic and Inflammatory Component of Postoperative Pain D Neychev,Folia Med (Plovdiv) ,2020
Calcitonin gene-related peptide levels in tear fluid are elevated in migraine patients compared to healthy controls Katharina Kamm,Cephalalgia,2019
3D printing mesoporous bioactive glass/sodium alginate/gelatin sustained release scaffolds for bone repair Wu J, et al,Journal of Biomaterials Applications,2018
Fasting serum CGRP levels are related to calcium concentrations, but cannot be elevated by short-term calcium/vitamin D supplementation. Hu F.et al,Neuropeptides.,2015
Plasma level of calcitonin gene-related peptide in patients with polycystic ovary syndrome and its relationship to hormonal and metabolic parameters Zhe Zhang et al,Peptides,2012

■ Customer Reviews

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■ Q&A

Can I test this product with the Cerebrospinal fluid sample?

Thanks for your inquiry. CGRP is a neuropeptide consisting of 37 amino acids.It is widespread in the central and peripheral nervous system.
There were reports saying that RIA was used to detect the level of CGRP in Cerebrospinal fluid
Regarding elisa kit, sorry we did not ever validate on Cerebrospinal fluid samples before.
We suggest you do a pre-testing first.

Could you disclose what capture antibody is used in the kit?

Thanks for your inquiry.
CSB-E08210h
Capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.

Function (From Uniprot)	CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
Gene References into Functions	The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115 Keratinocyte-derived CGRPbeta may modulate epidermal homeostasis through autocrine/paracrine signaling and may contribute to chronic pain under pathological conditions. PMID: 21641113 Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells. PMID: 19192276
Subcellular Location	Secreted.
Protein Families	Calcitonin family
Database Links	HGNC: 1438 OMIM: 114160 KEGG: hsa:797 STRING: 9606.ENSP00000346017 UniGene: Hs.534305

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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