Human calcitonin gene related peptide,CGRP ELISA Kit

Code CSB-E08210h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CGRP ELISA Kit was designed for the quantitative measurement of Human CGRP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

Target Name calcitonin gene related peptide,CGRP
Alternative Names Beta type CGRP ELISA Kit; Beta-CGRP ELISA Kit; Beta-type CGRP ELISA Kit; CALC2 ELISA Kit; Calcb ELISA Kit; CALCB_HUMAN ELISA Kit; Calcitonin gene related peptide 2 ELISA Kit; Calcitonin gene related peptide II ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; Calcitonin related polypeptide; beta ELISA Kit; CGRP II ELISA Kit; CGRP-II ELISA Kit; CGRP2 ELISA Kit; CGRPII ELISA Kit; FLJ30166 ELISA Kit
Abbreviation CGRP
Uniprot No. P10092
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-92
1:2Average %96
Range %91-105
1:4Average %97
Range %92-110
1:8Average %90
Range %86-98
Recovery
The recovery of human CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9690-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1002.660 2.511 2.586 2.432
502.080 2.152 2.116 1.962
251.451 1.366 1.409 1.255
12.50.795 0.744 0.770 0.616
6.250.491 0.523 0.507 0.353
3.120.315 0.325 0.320 0.166
1.560.248 0.244 0.246 0.092
00.157 0.151 0.154  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews

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 Q&A
Q:

Can I test this product with the Cerebrospinal fluid sample?

A:
Thanks for your inquiry. CGRP is a neuropeptide consisting of 37 amino acids.It is widespread in the central and peripheral nervous system.
There were reports saying that RIA was used to detect the level of CGRP in Cerebrospinal fluid
Regarding elisa kit, sorry we did not ever validate on Cerebrospinal fluid samples before.
We suggest you do a pre-testing first.
Q:

Could you disclose what capture antibody is used in the kit?

A:
Thanks for your inquiry.
CSB-E08210h
Capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.

Target Background

Function
(From Uniprot)
CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
Gene References into Functions
  1. The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115
  2. Keratinocyte-derived CGRPbeta may modulate epidermal homeostasis through autocrine/paracrine signaling and may contribute to chronic pain under pathological conditions. PMID: 21641113
  3. Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells. PMID: 19192276
Subcellular Location Secreted.
Protein Families Calcitonin family
Database Links

HGNC: 1438

OMIM: 114160

KEGG: hsa:797

STRING: 9606.ENSP00000346017

UniGene: Hs.534305

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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