Human cardiotrophln 1,CT-1 ELISA Kit

Code CSB-E08454h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cardiotrophin 1
Alternative Names Cardiophin 1 ELISA Kit; Cardiophin1 ELISA Kit; Cardiotrophin I ELISA Kit; Cardiotrophin-1 ELISA Kit; Cardiotrophin1 ELISA Kit; CardiotrophinI ELISA Kit; CT 1 ELISA Kit; CT-1 ELISA Kit; CT1 ELISA Kit; CTF 1 ELISA Kit; Ctf1 ELISA Kit; CTF1_HUMAN ELISA Kit
Abbreviation CTF1
Uniprot No. Q16619
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CT-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 87-105
1:2 Average % 95
Range % 90-102
1:4 Average % 89
Range % 83-97
1:8 Average % 95
Range % 85-102
Recovery
The recovery of human CT-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 92-99
EDTA plasma (n=4) 95 87-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.189 2.172 2.181 2.084
250 1.617 1.639 1.628 1.531
125 1.052 1.034 1.043 0.946
62.5 0.568 0.592 0.580 0.483
31.25 0.307 0.322 0.315 0.218
15.63 0.187 0.190 0.189 0.092
7.8 0.136 0.133 0.135 0.038
0 0.095 0.099 0.097  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
Gene References into Functions
  1. Data suggest that, in children with pediatric obesity, lifestyle weight-loss intervention results in down-regulation of serum cardiotrophin-1 (CTF1), interleukin-6 (IL6), and tumor necrosis factor-alpha (TNFA); expression of CTF1, IL6, and TNFA is also down-regulated in peripheral blood mononuclear cells after improvement in adiposity, body mass index, and waist-hip ratio. PMID: 28749076
  2. Cardiotrophin-1 levels were not an independent predictor of all-cause mortality in hemodialysis patients. PMID: 26787685
  3. We identify the cytokine cardiotrophin 1 (CT1) as a factor capable of recapitulating the key features of physiologic growth of the heart including transient and reversible hypertrophy of the myocardium, and stimulation of cardiomyocyte-derived angiogenic signals leading to increased vascularity PMID: 28785017
  4. a transcriptional factor, Myc-associated zinc finger protein (MAZ), plays an important role in ADAM10 transcription in response to CT-1 in neural stem/progenitor cells. PMID: 26867947
  5. CT-1 was found to be associated with Tn-I, which is used to detect myocardial damage after OPCAB surgery. CT-1 may also be used to detect myocardial damage. PMID: 26334851
  6. non-diabetic subjects who were overweight or obesity had significantly lower cardiotrophin-1 concentrations than those with normal weight, and both obesity and being overweight were inversely associated with cardiotrophin-1 levels. PMID: 26621340
  7. This paper will review many aspects of CT-1 physiological role in several organs and discuss data for consideration in therapeutic approaches. PMID: 26188636
  8. biliary epithelium-derived CT-1 may exert a profibrogenic potential in PCLD. PMID: 25919795
  9. Data show that cardiotrophin-1 is positively related to brachial-ankle pulse-wave velocity (baPWV) independent of traditional cardiometabolic risk factors for arterial stiffness. PMID: 25689900
  10. this study, even though preliminary and awaiting further confirmation by independent replication, provides first evidence that common genetic variation in CTF1 could contribute to insulin sensitivity in humans. PMID: 25025664
  11. Study correlates CT-1 levels with ambulatory and central BP, as well as with pulse wave velocity in patients with essential hypertension. PMID: 24401751
  12. CT-1 is differentially induced in the myocardium of infants with congenital cardiac defects depending on hypoxemia, and may mediate myocardial hypertrophy and dysfunction. PMID: 24344663
  13. Subjects with impaired glucose tolerance (IGT) and newly diagnosed diabetes (NDD) have significantly higher CT-1 concentrations than those with normal glucose tolerance. IGT and NDD are positively associated with CT-1 concentrations. PMID: 24054317
  14. exaggerated cardiomyocyte production of cardiotrophin-1 in response to increased left ventricular end-diastolic stress may contribute to fibrosis through stimulation of fibroblasts in heart failure of hypertensive origin. PMID: 24366078
  15. A reduction in serum CT-1 levels after a WL program. PMID: 23856329
  16. CT-1 induces the proteolytic potential in human aortic endothelial cells by upregulating MMP-1 expression. PMID: 23935888
  17. Cardiotrophin-1 may serve as both a biomarker of left ventricular hypertrophy and dysfunction in hypertensive patients, and is potential target for therapies aimed to prevent and treat hypertensive heart disease beyond blood pressure control. PMID: 22418690
  18. Hypoxia increased cardiotrophin-1 levels in cardiac cells through a direct regulation of CT-1 promoter by HIF-1alpha, protecting cells from apoptosis. PMID: 21771897
  19. CT-1 treatment of myofibroblasts was associated with increased phosphorylation of myosin light chain kinase via myosin light chain kinase to induce cell migration. PMID: 21572008
  20. the 1742(C/G) polymorphism of the human CT-1 gene is associated with LVH in hypertension, and the GG genotype may have a protective role PMID: 20683337
  21. This study aims to analyse cardiotrophin-1 (CT-1) and Tumor Necrosis Factor-alpha (TNF-alpha) plasma levels at rest and during exercise in elite athletes and healthy controls. PMID: 20053569
  22. Data show that both protein synthesis and intracellular transport are essential for cardiotrophin-1 induced TNFalpha expression. PMID: 20224758
  23. upregulation of cardiotrophin-1 in congestive heart failure PMID: 12234945
  24. Leukemia inhibitory factor (LIF), cardiotrophin-1, and oncostatin M share structural binding determinants in the immunoglobulin-like domain of LIF receptor PMID: 12707269
  25. Our study clearly demonstrates production of CT-1 in the postnatal and adult CNS, specifically by cell types comprising the blood-CSF barrier, and its accumulation in ventricular ependyma. PMID: 15219667
  26. Data show that cardiotrophin (CT-1) is a potent regulator of signaling in adipocytes in vitro and in vivo. PMID: 15339920
  27. CT-1 and TGF-beta 1 exert differential effects on myofibroblast proliferation and contraction in vitro. A balance of these effects may be important for normal cardiac wound healing. PMID: 17483238
  28. adipose tissue can be recognized as a source of CT-1, which could account for the high circulating levels of CT-1 in patients with metabolic syndrome X PMID: 17940213
  29. Increased expression of some IL-6 cytokine family members (oncostatin M, gp130, CT-1, LIF) in cutaneous inflammation might contribute to the promotion of hair loss. PMID: 17979974
  30. Perioperative plasma brain natriuretic peptide and cardiotrophin-1 measured in off-pump coronary artery bypass. PMID: 18609050
  31. Plasma cardiotrophin-1 is associated with progression of heart failure in hypertensive patients. PMID: 19155793
  32. Results indicate that transcription factors such as CTF1 can act to delimit chromatin domain boundaries at mammalian telomeres, thereby blocking the propagation of a silent chromatin structure. PMID: 19273604

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Subcellular Location Secreted.
Protein Families IL-6 superfamily
Tissue Specificity Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
Database Links

HGNC: 2499

OMIM: 600435

KEGG: hsa:1489

STRING: 9606.ENSP00000279804

UniGene: Hs.483811

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