Human chaperonin 10,CPN10 ELISA Kit

Instructions
Code CSB-E09917h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name heat shock 10kDa protein 1 (chaperonin 10)
Alternative Names 10 kDa chaperonin ELISA Kit; 10 kDa heat shock protein mitochondrial ELISA Kit; 10 kDa heat shock protein; mitochondrial ELISA Kit; CH10_HUMAN ELISA Kit; Chaperonin 10 ELISA Kit; Chaperonin 10 homolog ELISA Kit; CPN10 ELISA Kit; cpn10 homolog ELISA Kit; Early pregnancy factor ELISA Kit; Early-pregnancy factor ELISA Kit; EPF ELISA Kit; GROES ELISA Kit; GroES homolog ELISA Kit; Heat shock 10kD protein 1 chaperonin 10 ELISA Kit; Heat shock 10kDa protein 1 ELISA Kit; Heat shock 10kDa protein 1 chaperonin 10 ELISA Kit; Heat shock protein family E (Hsp10) member ELISA Kit; Heat-shock 10-kD protein ELISA Kit; Hsp10 ELISA Kit; Hspe1 ELISA Kit
Abbreviation HSPE1
Uniprot No. P61604
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.16 ng/mL-10 ng/mL
Sensitivity 0.04 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CPN10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 88-94    
1:2 Average % 102    
Range % 97-107    
1:4 Average % 94    
Range % 87-98    
1:8 Average % 102    
Range % 98-106    
Recovery
The recovery of human CPN10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 83-94    
EDTA plasma (n=4) 92 87-96    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
               
               
               
               
               
               
               
               
               
               
               
               
               
ng/ml OD1 OD2 Average Corrected    
10 2.688 2.579 2.634 2.525    
5 2.154 2.054 2.104 1.995    
2.5 1.365 1.302 1.334 1.225    
1.25 0.742 0.734 0.738 0.629    
0.63 0.417 0.405 0.411 0.302    
0.31 0.274 0.266 0.270 0.161    
0.16 0.186 0.183 0.185 0.076    
0 0.110 0.108 0.109      
               
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix
Gene References into Functions
  1. elevated expression of HSP10 protein inhibits apoptosis and associates with poor prognosis of astrocytoma PMID: 29028811
  2. miR-146a, miR-146b, and miR-155 are exerting anti-inflammatory properties by down-regulating IL-6 and IL-8, and influencing the expression of HSP10 in the activated endothelium PMID: 28662100
  3. High expression of HSP10 is negatively associated with estrogen receptor/progesterone receptor status and might be a novel independent biomarker for poor prognosis in invasive ductal breast carcinoma. PMID: 27993580
  4. EPF induces the differentiation of regulatory T cells and increases their immunosuppressive activities. PMID: 27840373
  5. Cpn10 has a role in the spatial regulation of NPAT signaling PMID: 26429916
  6. Hsp10 has a role in nuclear localization and lung cells response to cigarette smoke PMID: 25355063
  7. Data show that that in presence of 300 mg/mL Ficoll the thermodynamic stability of each cpn10 monomer increases by over 30%, whereas the interfaces are stabilized by less than 10%. PMID: 21375247
  8. HSP10 protein was detected only in oocytes from human preantral follicles, whereas in antral follicles, it was localised in oocytes, granulosa cells, theca cells and stroma cells. PMID: 19903031
  9. Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. PMID: 11898127
  10. The low stability of the monomeric unit suggests that folding and assembly reactions for cpn10 are coupled. PMID: 12220543
  11. Cpn10 and placental lactogen are capable of stimulating the synthesis of type I collagen by human osteoblasts in culture PMID: 12703979
  12. Identification of amino acids important for the assembly of the cpn10 heptamer. PMID: 14525625
  13. complex mechanisms are involved in the protection by hsp10 against simulated ischemia and reoxygenation-induced myocyte death PMID: 15059967
  14. The HSP10 plays a role in bone marrow cell differentiation other than being a mitochondrial co-chaperonin. PMID: 15590416
  15. Chaperonin 10 and calgranulin A are identified as markers for diagnosis and screening of endometrial carcinoma. PMID: 15816004
  16. the cpn10 interfaces can adapt to structural alterations without loss of either subunit-subunit affinity or heptamer specificity PMID: 15978542
  17. biophysical analysis of dissociation equilibrium for the heptameric co-chaperonin proteins 10 from Aquifex aeolicus and human mitochondria PMID: 16100270
  18. proteomic analysis of possible role of heat shock protein 10 in colorectal cancer PMID: 16502466
  19. Results describe the time-resolved folding and assembly mechanism of the heptameric co-chaperonin protein 10 (cpn10) in vitro. PMID: 16979655
  20. Investigation of single-nucleotide variations in the Hsp10 gene and their disease-causing potential. PMID: 17072495
  21. In this review, we revise the involvement of Hsp10 in signal transduction pathways and its possible role in cancer etiology. PMID: 17278877
  22. Data show that Hhsp10 formed fibrils from only the acidic unfolded state and Core peptide regions of these protein fibrils were determined by proteolysis followed by a combination of Edman degradation and mass spectroscopy analyses. PMID: 18329043
  23. The effects of cpn 10 on cells of the oligodendrocyte lineage, were assessed. PMID: 18465204
  24. In patients with serous ovarian carcinomas, gene expression analysis coupled with immunohistochemistry allowed the identification of HSP10 as an independent factor of progression-free survival. PMID: 18500265
  25. Results describe a novel function of chaperonin 10 as a general differentiation factor, not limited to erythroid cells, and show how this biological effect is mediated by GSK-3alpha/beta. PMID: 19142874
  26. HSP10 was identified as a new autoantigen in both autoimmune pancreatitis and fulminant type 1 diabetes. PMID: 19520060

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Subcellular Location Mitochondrion matrix
Protein Families GroES chaperonin family
Database Links

HGNC: 5269

OMIM: 600141

KEGG: hsa:3336

STRING: 9606.ENSP00000233893

UniGene: Hs.1197

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