Code | CSB-E08116h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 94 | ||||
Range % | 80-98 | |||||
1:2 | Average % | 95 | ||||
Range % | 91-105 | |||||
1:4 | Average % | 87 | ||||
Range % | 82-94 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-98 |
The recovery of human CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
EDTA plasma (n=4) | 93 | 90-100 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
800 | 2.453 | 2.504 | 2.478 | 2.478 | |||
400 | 1.275 | 1.287 | 1.281 | 1.281 | |||
280 | 0.756 | 0.748 | 0.752 | 0.752 | |||
144 | 0.395 | 0.410 | 0.402 | 0.402 | |||
56 | 0.200 | 0.205 | 0.203 | 0.203 | |||
0 | 0.112 | 0.113 | 0.113 | 0.113 |
This Human CCK ELISA Kit was designed for the quantitative measurement of Human CCK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 56 pg/mL-800 pg/mL and the sensitivity is 20 pg/mL.
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I would like to ask you if you know what is the most common sample dilution in human CCK elisa kit?