Human cytotoxin-associated protein(CagA) antibody (IgG) ELISA kit

Cytotoxin-associated gene A (CagA) is a virulence factor produced by certain strains of Helicobacter pylori, a pathogenic bacterium associated with gastric ulcers and gastric cancer. CagA protein is injected into host gastric epithelial cells through a type IV secretion system, where it undergoes tyrosine phosphorylation and disrupts normal cellular signaling pathways. CagA-positive H. pylori strains represent a major risk factor for severe gastric pathology. This makes CagA antibodies valuable biomarkers for determining infection status and disease progression in clinical and research settings.

The assay uses a detection wavelength of 450 nm and requires 50-100 μl sample volume per test. The complete assay procedure takes 1-5 hours, making it suitable for routine laboratory applications in Homo sapiens research studies.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research focused on measuring antibody responses to cytotoxin-associated protein in human serum samples. The applications center on quantifying immunoglobulin G levels against CagA protein in clinical research contexts, particularly in studies examining gastric cancer patients.

• Gastric cancer research - Measurement of CagA-specific antibody levels in serum samples from gastric cancer patients to determine immune responses to this bacterial virulence factor
• Biomarker studies - Quantification of anti-CagA IgG antibodies as potential indicators in disease-related studies involving Helicobacter pylori infection
• Clinical immunology - Analysis of specific antibody responses in human serum to evaluate immune system interactions with bacterial cytotoxin-associated proteins