Human glucagon-like peptide-1,GLP-1 ELISA Kit

Code CSB-E08119h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glucagon-like peptide-1,GLP-1
Alternative Names N/A
Abbreviation GLP-1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 2.3 ng/mL-40 ng/mL
Sensitivity 1.4 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 87-107  
1:2 Average % 96  
Range % 90-106  
1:4 Average % 95  
Range % 86-105  
1:8 Average % 96  
Range % 89-100  
The recovery of human GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-101  
EDTA plasma (n=4) 95 89-103  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
2.3 0.063 0.061 0.062    
4.6 0.169 0.172 0.171    
9.1 0.398 0.384 0.391    
22.9 0.861 0.872 0.867    
40 1.660 1.653 1.657    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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