Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

Code CSB-E08118m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Mouse glucagon-like peptide-1 (GLP-1) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of mouse GLP-1in many biological fluids, including serum, plasma, or tissue homogenates. GLP-1 is a gut-derived peptide secreted from intestinal L-cells following a meal. The action of GLP-1 is through the binding of a specific GLP-1 receptor, which is expressed in various tissues, including pancreatic beta cells, pancreatic ducts, and gastric mucosa. GLP-1 exerts its main effect by enhancing glucose-dependent insulin production. GLP-1 has also been demonstrated to delay gastric emptying, suppress inappropriate post-meal glucagon release, and increase satiety.

This assay employs the Sandwich-ELISA technique, in which GLP-1 in the samples or standards is sandwiched between pre-coated an anti-mouse GLP-1 and Biotin-labeled GLP-1 antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate. The intensity of the color is positively correlated to the amount of GLP-1 in the sample. The kit has a detection range of 62.5 ng/mL-4000 ng/mL, excellent sensitivity, high specificity, good linearity, precision low than 10%, high recovery, good lot-to-lot consistency. Get access to more details from the product instructions.

Alternative Names
N/A
Abbreviation GLP-1
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates.
Detection Range 125 pg/ml-8000 pg/ml.
Sensitivity 31.25 pg/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Materials provided
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse GLP-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled GLP-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
4.8 - 4 reviews

Submit a Review here

Sample type: Serum

Sample species: Mouse

Review: Administration of GLP-1, reserpine and polytherapy with GLP-1 and reserpine increases GLP-1 levels in diabetic mice. Note that GLP-1 levels in mice after GLP-1/reserpine polytherapy are significantly higher compared to both diabetic mice and diabetic mice after GLP-1 and reserpine monotherapy.

By Anonymous

Sample type: Biological fluids(bronchoalveolar lavage fluid)

Sample species: Mouse

Review: Liraglutide restored LPS-induced decrease of GLP-1 in serum, b reduced LPS-induced increase of GLP-1 in bronchoalveolar lavage fluid (BALF).

By Anonymous

Sample type: Serum

Sample species: Mouse

Review: Levels of serum GLP-1. Data are presented as mean ± SEM. Scale bar, 50 μm. **p<0.01 and *p<0.05 versus model group, respectively. Normal, normal mice group; Model, T2D mice group; Met, T2D mice group administrated with metformin at 200 mg/kg/day; GXG-H, T2D mice group administrated with 200 mg/kg/day; GXG-L, T2D mice group administrated with 50 mg/kg/day. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).

By Anonymous

Sample type: Plasma (anticoagulant)

Sample species: Mouse

Review: Plasma levels of GLP-1 by ELISA.

By Anonymous

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